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Mouse IgG2A Isotype Control

Catalog #: MAB0031
18 Citations 2 Reviews Datasheet
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MAB0031
Detection of Mouse IgG Isotype Control by Flow Cytometry
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Citations (18)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IgG2A Isotype Control Summary

Specificity
Recognizes KLH (Keyhole Limpet Hemocyanin).  In immunohistochemistry or flow cytometry of a variety of cell types or tissues, no background staining is observed.
Source
Monoclonal Mouse IgG2A Clone # 133304
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Control
Negative control for flow cytometry, immunohistochemistry, or immunocytochemistry experiments. Use at the same concentration as the detection antibody.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Control Detection of Mouse IgG Isotype Control by Flow Cytometry View Larger

Detection of Mouse IgG Control by Flow Cytometry HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human ULBP-2/5/6 Monoclonal Antibody (Catalog # MAB1298, filled histogram) or Mouse IgG Isotype Control Antibody (Catalog # MAB0031, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog F0101B).

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG2A

IgG is the most abundant of the five classes of mammalian immunoglobulins in serum. IgG molecules (~150 kDa) consist of two ~50 kDa heavy chains and two 25 kDa light chains connected by disulfide bonds. Antibody specificity is conferred by a combination of somatic rearrangement and hypermutation within variable regions. Mouse IgG isotypes IgG1, IgG2a and IgG2b share approximately 64-78% amino acid sequence identity within the heavy chain constant region domains (CH1, CH2 and CH3) and may vary in their interaction with complement, NK and mast cells, induction or inhibition by specific cytokines, and ability to cross the placenta.

Long Name
Immunoglobulin G2A Fc
Alternate Names
IgG2A

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Citations for Mouse IgG2A Isotype Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. Human amnion epithelial cells induced to express functional cystic fibrosis transmembrane conductance regulator.
    Authors: Murphy, Sean V, Lim, Rebecca, Heraud, Philip, Cholewa, Marian, Le Gros, Mark, de Jonge, Martin D, Howard, Daryl L, Paterson, David, McDonald, Courtney, Atala, Anthony, Jenkin, Graham, Wallace, Euan M
    PLoS ONE, 2012-09-28;7(9):e46533.
  2. Laser microdissection, proteomics, and multiplex immunohistochemistry: a bumpy ride into the study of paraffin-embedded fetal and pediatric lung tissues
    Authors: Luis M. Cardoso dos Santos, Yannick Avila, Domitille Schvartz, Anne-Laure Rougemont, Marie-Luce Bochaton-Piallat, Isabelle Ruchonnet-Metrailler
    Frontiers in Medicine
  3. Cholesterol depletion decreases adhesion of non-small cell lung cancer (NSCLC) cells to E-selectin
    Authors: Amina Mohammadalipour, Christian A. Showalter, Harrison T. Muturi, Amir M. Farnoud, Sonia M. Najjar, Monica M. Burdick
    American Journal of Physiology-Cell Physiology
  4. Immunoaffinity-Based Isolation of Melanoma Cell-Derived and T Cell-Derived Exosomes from Plasma of Melanoma Patients
    Authors: Mondal SK, Whiteside TL.
    Methods in Molecular Biology
  5. E2F6 initiates stable epigenetic silencing of germline genes during embryonic development
    Authors: T Dahlet, M Truss, U Frede, H Al Adhami, AF Bardet, M Dumas, J Vallet, J Chicher, P Hammann, S Kottnik, P Hansen, U Luz, G Alvarez, G Auclair, J Hecht, PN Robinson, C Hagemeier, M Weber
    Nature Communications, 2021-06-11;12(1):3582.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP Control
  6. JAK3 Is Expressed in the Nucleus of Malignant T Cells in Cutaneous T Cell Lymphoma (CTCL)
    Authors: CK Vadivel, M Gluud, S Torres-Rus, L Boding, A Willerslev, TB Buus, TK Nielsen, JL Persson, CM Bonefeld, C Geisler, T Krejsgaard, AT Fuglsang, N Odum, A Woetmann
    Cancers, 2021-01-14;13(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Induction of Chemokines by Hepatitis C Virus Proteins: Synergy of the Core Protein with Interleukin-1 beta and Interferon-gamma in Liver Bystander Cells
    Authors: Sara Abouelasrar Salama, Mieke Gouwy, Alexandra De Zutter, Noëmie Pörtner, Lotte Vanbrabant, Nele Berghmans et al.
    Journal of Interferon & Cytokine Research
  8. Effects of cisplatin on the proliferation, invasion and apoptosis of breast cancer cells following beta ‑catenin silencing
    Authors: Xidan Zhu, Jia Feng, Wenguang Fu, Xiaojia Shu, Xue Wan, Jinbo Liu
    International Journal of Molecular Medicine
  9. Aberrantly elevated Bmi1 promotes cervical cancer tumorigenicity and tumor sphere formation via enhanced transcriptional regulation of Sox2 genes
    Authors: R Xu, L Chen, WT Yang
    Oncol. Rep., 2019-06-06;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  10. EHMT2 directs DNA methylation for efficient gene silencing in mouse embryos
    Authors: Ghislain Auclair, Julie Borgel, Lionel A. Sanz, Judith Vallet, Sylvain Guibert, Michael Dumas et al.
    Genome Research
  11. Recognition of bacterial signal peptides by mammalian formyl peptide receptors: a new mechanism for sensing pathogens.
    Authors: Bufe B, Schumann T, Kappl R, Bogeski I, Kummerow C, Podgorska M, Smola S, Hoth M, Zufall F
    J Biol Chem, 2015-01-20;290(12):7369-87.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. Structure of acidic pH dengue virus showing the fusogenic glycoprotein trimers.
    Authors: Zhang X, Sheng J, Austin S, Hoornweg T, Smit J, Kuhn R, Diamond M, Rossmann M
    J Virol, 2014-10-29;89(1):743-50.
    Species: Hamster
    Sample Types: Whole Cells
    Applications: Isotype Control
  13. In situ characterization of intrahepatic non-parenchymal cells in PSC reveals phenotypic patterns associated with disease severity.
    Authors: Berglin L, Bergquist A, Johansson H, Glaumann H, Jorns C, Lunemann S, Wedemeyer H, Ellis E, Bjorkstrom N
    PLoS ONE, 2014-08-20;9(8):e105375.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Isotype Control
  14. Neural Crest Cells Isolated from the Bone Marrow of Transgenic Mice Express JCV T-Antigen
    Authors: Jennifer Gordon, Ilker K. Sariyer, Marisol De La Fuente-Granada, Brian J. Augelli, Jessica Otte, S. Ausim Azizi et al.
    PLoS ONE
  15. Development of a highly protective combination monoclonal antibody therapy against Chikungunya virus.
    Authors: Pal, Pankaj, Dowd, Kimberly, Brien, James D, Edeling, Melissa, Gorlatov, Sergey, Johnson, Syd, Lee, Iris, Akahata, Wataru, Nabel, Gary J, Richter, Mareike, Smit, Jolanda, Fremont, Daved H, Pierson, Theodore, Heise, Mark T, Diamond, Michael
    PLoS Pathog, 2013-04-18;9(4):e1003312.
    Species: Primate - Chlorocebus aethiops (African Green Monkey)
    Sample Types: Whole Cells
    Applications: Functional Assay
  16. CXCR7 protein is not expressed on human or mouse leukocytes.
    Authors: Berahovich RD, Zabel BA, Penfold ME, Lewen S, Wang Y, Miao Z, Gan L, Pereda J, Dias J, Slukvin II, McGrath KE, Jaen JC, Schall TJ
    J. Immunol., 2010-10-01;185(9):5130-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Susceptibility of human testis to human immunodeficiency virus-1 infection in situ and in vitro.
    Authors: Roulet V, Satie AP, Ruffault A, Le Tortorec A, Denis H, Guist'hau O, Patard JJ, Rioux-Leclerq N, Gicquel J, Jegou B, Dejucq-Rainsford N
    Am. J. Pathol., 2006-12-01;169(6):2094-103.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  18. Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
    Authors: Ebert EC, Mehta V
    Cell. Immunol., 2006-08-17;241(1):7-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Mouse IgG2A Isotype Control

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Mouse IgG2A Isotype Control
By Anonymous on 08/17/2016
Application: Immunocytochemistry/Immunofluorescence Sample Tested: ?TC-6 mouse beta cell insulinoma cell line Species: Human and Mouse
Mouse IgG2A Isotype Control
By Anonymous on 02/22/2016
Application: Flow Sample Tested: Human Placenta Species: Human and Mouse