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Mouse IgG2A APC-conjugated Antibody

Catalog #: IC003A
20 Citations Datasheet
Catalog # Availability Size / Price Qty
IC003A
Detection of Mouse IgG Isotype Control by Flow Cytometry
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Citations (20)
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Mouse IgG2A APC-conjugated Antibody Summary

Specificity
Selected for use as mouse IgG2A isotype control for flow cytometry applications.
Source
Monoclonal Mouse IgG2A Clone # 20102
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Control Detection of Mouse IgG Isotype Control by Flow Cytometry View Larger

Detection of Mouse IgG Control by Flow Cytometry Neuro-2A mouse neuroblastoma cell line was stained with Mouse Anti-Human/Mouse Orexin R2/HCRTR2 APC-conjugated Monoclonal Antibody (Catalog # IC5246A, filled histogram) or Mouse IgG APC-conjugated Isotype Control Antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2A Fc
Alternate Names
IgG2A

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Citations for Mouse IgG2A APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. TACSTD2 upregulation is an early reaction to lung infection
    Authors: S Lenárt, P Lenárt, L Knopfová, H Kotasová, V Pelková, V Sedláková, O Vacek, J Pokludová, V ?an, J Šmarda, K Sou?ek, A Hampl, P Beneš
    Oncogene, 2022-06-10;12(1):9583.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Effective targeting of breast cancer stem cells by combined inhibition of Sam68 and Rad51
    Authors: A Turdo, M Gaggianesi, S Di Franco, V Veschi, C D'Accardo, G Porcelli, M Lo Iacono, I Pillitteri, F Verona, G Militello, A Zippo, V Poli, L Fagnocchi, S Beyes, S Stella, R Lattanzio, N Faldetta, VL Lentini, R Porcasi, G Pistone, MR Bongiorno, G Stassi, R De Maria, M Todaro
    Oncogene, 2022-02-25;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Neuropilin-1 is over-expressed in claudin-low breast cancer and promotes tumor progression through acquisition of stem cell characteristics and RAS/MAPK pathway activation
    Authors: Yu Hin Tang, Anja Rockstroh, Kamil A. Sokolowski, Layla-Rose Lynam, Melanie Lehman, Erik W. Thompson et al.
    Breast Cancer Research
  4. miR-188-5p promotes oxaliplatin resistance by targeting RASA1 in colon cancer cells
    Authors: X Zhu, X Luo, Z Song, S Jiang, X Long, X Gao, X Xie, L Zheng, H Wang
    Oncology Letters, 2021-04-20;21(6):481.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. TNFR2 expression is a hallmark of human memory B cells with suppressive function
    Authors: O Ticha, P Slanina, L Moos, J Stichova, M Vlkova, I Bekeredjia
    European Journal of Immunology, 2021-03-08;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: FACS Control
  6. Recombinant immunotoxin targeting GPC3 is cytotoxic to H466 small cell lung cancer cells
    Authors: Ewelina Rodakowska, Aurelia Walczak‑Drzewiecka, Marta Borowiec, Michal Gorzkiewicz, Joanna Grzesik, Marcin Ratajewski et al.
    Oncology Letters
  7. Detection of urinary podocytes by flow cytometry in idiopathic membranous nephropathy
    Authors: A Mella, I Deambrosis, S Mingozzi, L Colla, M Burdese, F Giaretta, S Bruno, G Camussi, E Boaglio, C Dolla, R Clari, L Biancone
    Sci Rep, 2020-10-01;10(1):16362.
    Species: Human
    Sample Types: Urine
    Applications: ICC
  8. Soluble silica stimulates osteogenic differentiation and gap junction communication in human dental follicle cells
    Authors: P Uribe, A Johansson, R Jugdaohsin, JJ Powell, C Magnusson, M Davila, A Westerlund, M Ransjö
    Sci Rep, 2020-06-18;10(1):9923.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  9. Chemokine Receptor CCR7 Triggers an Endomembrane Signaling Complex for Spatial Rac Activation
    Authors: JM Laufer, MA Hauser, I Kindinger, V Purvanov, A Pauli, DF Legler
    Cell Rep, 2019-10-22;29(4):995-1009.e6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. CD206+ tumor-associated macrophages promote proliferation and invasion in oral squamous cell carcinoma via EGF production
    Authors: ASMR Haque, M Moriyama, K Kubota, N Ishiguro, M Sakamoto, A Chinju, K Mochizuki, T Sakamoto, N Kaneko, R Munemura, T Maehara, A Tanaka, JN Hayashida, S Kawano, T Kiyoshima, S Nakamura
    Sci Rep, 2019-10-10;9(1):14611.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Engineering of Nanobodies Recognizing the Human Chemokine Receptor CCR7
    Authors: BD Jakobs, L Spannagel, V Purvanov, E Uetz-von A, C Matti, DF Legler
    Int J Mol Sci, 2019-05-27;20(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Melatonin restricts the viability and angiogenesis of vascular endothelial cells by suppressing HIF-1 alpha /ROS/VEGF
    Authors: Jiao Cheng, Hui‑Li Yang, Chun‑Jie Gu, Yu‑Kai Liu, Jun Shao, Rui Zhu et al.
    International Journal of Molecular Medicine
  13. Microenvironment in neuroblastoma: isolation and characterization of tumor-derived mesenchymal stromal cells
    Authors: G Pelizzo, V Veschi, M Mantelli, S Croce, V Di Benedet, P D'Angelo, A Maltese, L Catenacci, T Apuzzo, E Scavo, A Moretta, M Todaro, G Stassi, MA Avanzini, V Calcaterra
    BMC Cancer, 2018-11-27;18(1):1176.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  14. Mutational analysis of AKT1 and PIK3CA in intraductal papillomas of the breast with special reference to cellular components
    Authors: C Mishima, N Kagara, JI Ikeda, E Morii, T Miyake, T Tanei, Y Naoi, M Shimoda, K Shimazu, SJ Kim, S Noguchi
    Am. J. Pathol., 2018-02-16;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Fibroblast Heterogeneity and Immunosuppressive Environment in Human Breast Cancer
    Authors: A Costa, Y Kieffer, A Scholer-Da, F Pelon, B Bourachot, M Cardon, P Sirven, I Magagna, L Fuhrmann, C Bernard, C Bonneau, M Kondratova, I Kuperstein, A Zinovyev, AM Givel, MC Parrini, V Soumelis, A Vincent-Sa, F Mechta-Gri
    Cancer Cell, 2018-02-15;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: FACS
  16. High-throughput immunophenotypic characterization of bone marrow- and cord blood-derived mesenchymal stromal cells reveals common and differentially expressed markers: identification of angiotensin-converting enzyme (CD143) as a marker differentially expressed between adult and perinatal tissue sources
    Authors: E Amati, O Perbellini, G Rotta, M Bernardi, K Chieregato, S Sella, F Rodeghiero, M Ruggeri, G Astori
    Stem Cell Res Ther, 2018-01-16;9(1):10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Neuropilin-1 is upregulated in the adaptive response of prostate tumors to androgen-targeted therapies and is prognostic of metastatic progression and patient mortality
    Authors: BW Tse, M Volpert, E Ratther, N Stylianou, M Nouri, K McGowan, ML Lehman, SJ McPherson, M Roshan-Mon, MS Butler, J Caradec, CY Gregory-Ev, J McGovern, R Das, M Takhar, N Erho, M Alshalafa, E Davicioni, EM Schaeffer, RB Jenkins, AE Ross, RJ Karnes, RB Den, L Fazli, PA Gregory, ME Gleave, ED Williams, PS Rennie, R Buttyan, JH Gunter, LA Selth, PJ Russell, CC Nelson, BG Hollier
    Oncogene, 2017-01-16;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Noncanonical Wnt signaling orchestrates early developmental events toward hematopoietic cell fate from human embryonic stem cells.
    Authors: Vijayaragavan K, Szabo E, Bosse M, Ramos-Mejia V, Moon RT, Bhatia M
    Cell Stem Cell, 2009-03-06;4(3):248-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  19. Systematic analysis of the human tumor cell binding to human vs. murine E- and P-selectin under static vs. dynamic conditions
    Authors: Starzonek S, Maar H, Labitzky V et Al.
    Glycobiology
  20. Eicosapentaenoic acid in combination with EPHA2 inhibition shows efficacy in preclinical models of triple-negative breast cancer by disrupting cellular cholesterol efflux
    Authors: AM Torres-Ado, H Vitrac, Y Qi, L Tan, KR Levental, YY Fan, P Yang, RS Chapkin, BL Eckhardt, NT Ueno
    Oncogene, 2018-11-20;0(0):.

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