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Mouse IgG2B Antibody

Catalog #: MAB0043 Datasheet
Catalog # Availability Size / Price Qty
MAB0043-100
Detection of Mouse IgG2B Primary Antibody by Flow Cytometry
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Mouse IgG2B Antibody Summary

Species Reactivity
Mouse
Specificity
The specificity of this antibody was confirmed by direct ELISA on mouse IgG2B monoclonal antibodies, and it does not cross-react with IgG1, IgG2A, IgG3, IgM, IgA, or IgE antibodies. By flow cytometry, this isotype-specific secondary antibody detects primary antibodies of the mouse IgG2B isotype, and does not detect primary antibodies of mouse IgG1, IgG2A, or IgG3 class.
Source
Monoclonal Rat IgG1 Clone # 332723
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Cocktail of mouse IgG2B and IgG3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Mouse IgG2B Primary Antibody by Flow Cytometry View Larger

Detection of Mouse IgG2B Primary Antibody by Flow Cytometry Human peripheral blood lymphocytes were stained with A) Mouse Anti-Human CD8 alpha Monoclonal Antibody (Catalog # MAB1509, Mouse IgG2B, filled histogram) or isotype control (Catalog # MAB0041, open histogram), or B) Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB3791, Mouse IgG2A, filled histogram) or isotype control (Catalog # MAB003, open histogram), followed by Rat Anti-Mouse IgG2B isotype-specific secondary antibody (Catalog # MAB0043) and then Allophycocyanin-conjugated Anti-Rat IgG SecondaryAntibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2B Fc
Alternate Names
IgG2B

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