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Mouse IgG2A Antibody

Catalog #: MAB0033 Datasheet
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB0033-100
Detection of Mouse IgG2APrimary Antibodies by Western Blot.
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Mouse IgG2A Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IgG2A in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with mouse IgG1, mouse IgG2B, and mouse IgG3 was observed.
Source
Monoclonal Rabbit IgG Clone # 2214A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse IgG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse IgG<sub>2A</sub>Primary Antibodies by Western Blot. View Larger

Detection of Mouse IgG2APrimary Antibodies by Western Blot. Western blot shows lysates of HepG2 and Jurkat cell lines. PVDF membrane was probed with anti-HSP90 (Catalog # MAB3286, Mouse IgG2Bisotype), anti-HSP60 (Catalog # MAB1800, Mouse IgG2Bisotype), anti-GAPDH (Catalog # MAB5718, Mouse IgG1isotype), anti-Peroxiredoxin 6 (Catalog # MAB3490, Mouse IgG3isotype), and anti-SOD1 (Catalog # MAB3418, Mouse IgG2Aisotype). The left panel was then probed with 0.5 µg/mL of Rabbit Anti-Mouse IgG2A(Catalog # MAB0033) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for IgG2Aisotype antibody at approximately 18 kDa (as indicated). The right panel was probed with HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) but not Rabbit Anti-Mouse IgG2AMonoclonal Antibody (Catalog # MAB0033). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of Mouse IgG<sub>2A</sub>Primary Antibody by Anti-Mouse IgG<sub>2B</sub>specific Secondary Antibody. View Larger

Detection of Mouse IgG2APrimary Antibody by Anti-Mouse IgG2Bspecific Secondary Antibody. Human PBMC lymphocytes were stained with Mouse anti-Human CD3e Monoclonal Antibody (Catalog # MAB100, Mouse IgG1isotype), Mouse anti-Human CD4 Monoclonal Antibody (Catalog # MAB3791, Mouse IgG2Aisotype), or Mouse anti-Human CD8a Monoclonal Antibody (Catalog # MAB1509, Mouse IgG2Bisotype), followed by Rabbit anti-Mouse IgG2A-specific secondary antibody (Catalog # MAB0033) then PE-conjugated Goat anti-Rabbit secondary antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2A Fc
Alternate Names
IgG2A

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