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Mouse IGF-I/IGF-1 Biotinylated Antibody

Catalog #: BAF791
6 Citations 1 Review Datasheet
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BAF791
Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (1)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IGF-I/IGF-1 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IGF-I/IGF-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human IGF-I/IGF-1 is observed and less than 0.1% cross-reactivity with recombinant mouse IGF-II/IGF-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IGF-I/IGF-1
Gly33-Ala102
Accession # Q8CAR0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG)

Mouse IGF-I/IGF-1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse IGF-I/IGF-1 Antibody (Catalog # MAB791)

Standard: Recombinant Mouse IGF-I/IGF-1 Protein, CF (Catalog # 791-MG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGF-I/IGF-1

Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Mouse IGF-I is synthesized as two precursor isoforms with alternate N- and C-terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature mouse IGF-I shares 94% and 99% aa sequence identity with human and rat IGF-I, respectively (2), and exhibits cross-species activity. It shares 60% aa sequence identity with mature mouse IGF‑II. Circulating IGF-I is produced by hepatocytes, while local IGF-I is produced by many other tissues in which it has paracrine effects (1). IGF-I induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-I regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4-7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-I binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (9). IGF-I association with IGF binding proteins increases its plasma half-life and modulates its interactions with receptors (10).

References
  1. Philippou, A. et al. (2007) In Vivo 21:45.
  2. Bell, G.I. et al. (1986) Nucleic Acids Res. 14:7873.
  3. Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890.
  4. Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620.
  5. Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577.
  6. Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275.
  7. Malemud, C.J. (2007) Clin. Chim. Acta 375:10.
  8. Samani, A.A. et al. (2007) Endocrine Rev. 28:20.
  9. LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302.
  10. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.
Long Name
Insulin-like Growth Factor I/Insulin-like Growth Factor 1
Entrez Gene IDs
3479 (Human); 16000 (Mouse); 24482 (Rat)
Alternate Names
IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C

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Citations for Mouse IGF-I/IGF-1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Cysteine induces longitudinal bone growth in mice by upregulating IGF-I
    Authors: PHIL-DONG MOON, MIN-HO KIM, HYUN-A OH, SUN-YOUNG NAM, NA-RA HAN, HYUN-JA JEONG et al.
    International Journal of Molecular Medicine
  2. Spatial distribution of insulin-like growth factor binding protein-2 following hypoxic-ischemic injury.
    Authors: Fletcher L, Isgor E, Sprague S, Williams L, Alajajian B, Jimenez D, Digicaylioglu M
    BMC Neurosci, 2013-12-21;14(0):158.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Detection)
  3. IGFBP-3 is a metastasis suppression gene in prostate cancer.
    Authors: Mehta HH, Gao Q, Galet C
    Cancer Res., 2011-06-22;71(15):5154-63.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development
  4. Evidence of a role for insulin-like growth factor binding protein (IGFBP)-3 in metabolic regulation.
    Authors: Yamada PM, Mehta HH, Hwang D
    Endocrinology, 2010-10-06;151(12):5741-50.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development
  5. Autologous bone marrow stromal cells genetically engineered to secrete an igf-I receptor decoy prevent the growth of liver metastases.
    Authors: Wang N, Fallavollita L, Nguyen L, Burnier J, Rafei M, Galipeau J, Yakar S, Brodt P
    Mol. Ther., 2009-04-14;17(7):1241-9.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  6. Dietary feeding of silibinin inhibits prostate tumor growth and progression in transgenic adenocarcinoma of the mouse prostate model.
    Authors: Raina K, Blouin MJ, Singh RP, Majeed N, Deep G, Varghese L, Glode LM, Greenberg NM, Hwang D, Cohen P, Pollak MN, Agarwal R
    Cancer Res., 2007-11-15;67(22):11083-91.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development

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Mouse IGF‑I Biotinylated Antibody
By Alexandria Schieber on 02/22/2016
Application: ELISA Sample Tested: Adipocytes,Adipose tissue,Large intestine,Liver tissue,Serum,Small intestine tissue Species: Bacterial and Mouse