Mouse HGF Antibody Summary
Gln33-Leu728
Accession # Q53WS5
Applications
Mouse HGF Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data

HGF in Mouse Liver. HGF was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2207) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

HGF in Mouse Skeletal Muscle. HGF was detected in perfusion fixed frozen sections of mouse skeletal muscle using Goat Anti-Mouse HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2207) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HGF
HGF, also known as Scatter Factor and Hepatopoietin A, is a pleiotropic protein in the Plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1‑5). Mouse HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide‑linked HGF with a 60 kDa alpha and 30 kDa beta chain. Alternate splicing generates an isoform that lacks the peptidase and the second, third, and fourth Kringle domains. Mouse HGF shares 91%‑95% aa sequence identity with bovine, canine, feline, human, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF-dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the upregulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin-1 alpha ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).
- Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
- Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
- Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3.
- Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501.
- Sasaki, M. et al. (1994) Biochem. Biophys. Res. Commun. 199:772.
- Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131.
- Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
- Corso, S. et al. (2005) Trends Mol. Med. 11:284.
- Maeshima, A. et al. (2000) Kid. Int. 58:1511.
- Montesano, R. et al. (1991) Cell 67:901.
- Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
- Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
- Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
- Mineo, R. et al. (1994) Endocrinology 145:4355.
- Urbanek, K. et al. (2005) Circ. Res. 97:663.
Product Datasheets
Citations for Mouse HGF Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
15
Citations: Showing 1 - 10
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LIFR recruits HGF-producing neutrophils to promote liver injury repair and regeneration
Authors: Y Deng, Z Zhao, M Sheldon, Y Zhao, H Teng, C Martinez, J Zhang, C Lin, Y Sun, F Yao, H Zhu, L Ma
bioRxiv : the preprint server for biology, 2023-03-21;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC -
Elimination of Radiation-Induced Senescence in the Brain Tumor Microenvironment Attenuates Glioblastoma Recurrence
Authors: Eliot Fletcher-Sananikone, Suman Kanji, Nozomi Tomimatsu, Luis Fernando Macedo Di Di Cristofaro, Rahul K. Kollipara, Debabrata Saha et al.
Cancer Research
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Vascular endothelium plays a key role in directing pulmonary epithelial cell differentiation
Authors: Jiayi Yao, Pierre J. Guihard, Xiuju Wu, Ana M. Blazquez-Medela, Melissa J. Spencer, Medet Jumabay et al.
Journal of Cell Biology
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Progesterone dose-dependently modulates hepatocyte growth factor production in 3T3-L1 mouse preadipocytes
Authors: T Ito, D Yamaji, A Kamikawa, MA Abd Eldaim, Y Okamatsu-O, A Terao, M Saito, K Kimura
Endocr. J., 2017-07-27;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Epigenetic regulation of HGF/Met receptor axis is critical for the outgrowth of bone metastasis from breast carcinoma
Authors: P Bendinelli, P Maroni, E Matteucci, MA Desiderio
Cell Death Dis, 2017-02-02;8(2):e2578.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Targeting Serglycin Prevents Metastasis in Murine Mammary Carcinoma
PLoS ONE, 2016-05-25;11(5):e0156151.
Species: Mouse
Sample Types: N/A
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MET is required for the recruitment of anti-tumoural neutrophils.
Authors: Finisguerra V, Di Conza G, Di Matteo M, Serneels J, Costa S, Thompson A, Wauters E, Walmsley S, Prenen H, Granot Z, Casazza A, Mazzone M
Nature, 2015-05-18;522(7556):349-53.
Species: Human, Mouse
Sample Types: In Vivo, Whole Cells
Applications: Neutralization -
Necrotic cell-derived high mobility group box 1 attracts antigen-presenting cells but inhibits hepatocyte growth factor-mediated tropism of mesenchymal stem cells for apoptotic cell death.
Authors: Vogel S, Borger V, Peters C, Forster M, Liebfried P, Metzger K, Meisel R, Daubener W, Trapp T, Fischer J, Gawaz M, Sorg R
Cell Death Differ, 2015-01-09;22(7):1219-30.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC, Neutralization -
Mesenchymal stem cells modulate albumin-induced renal tubular inflammation and fibrosis.
Authors: Wu H, Yiu W, Li R, Wong D, Leung J, Chan L, Zhang Y, Lian Q, Lin M, Tse H, Lai K, Tang S
PLoS ONE, 2014-03-19;9(3):e90883.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Bone marrow progenitor cell therapy-mediated paracrine regulation of cardiac miRNA-155 modulates fibrotic response in diabetic hearts.
Authors: Kishore, Raj, Verma, Suresh K, Mackie, Alexande, Vaughan, Erin E, Abramova, Tatiana, Aiko, Ito, Krishnamurthy, Prasanna
PLoS ONE, 2013-04-01;8(4):e60161.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Hepatocyte growth factor activator inhibitor type 1 is a suppressor of intestinal tumorigenesis.
Authors: Hoshiko S, Kawaguchi M, Fukushima T, Haruyama Y, Yorita K, Tanaka H, Seiki M, Inatsu H, Kitamura K, Kataoka H
Cancer Res, 2013-02-27;73(8):2659-70.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
c-Met and its ligand hepatocyte growth factor/scatter factor regulate mature B cell survival in a pathway induced by CD74.
Authors: Gordin M, Tesio M, Cohen S
J. Immunol., 2010-07-16;185(4):2020-31.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry, Neutralization -
Enhanced c-Met activity promotes G-CSF-induced mobilization of hematopoietic progenitor cells via ROS signaling.
Authors: Tesio M, Golan K, Corso S
Blood, 2010-06-28;117(2):419-28.
Species: Mouse
Sample Types: Plasma, Whole Cells
Applications: ELISA Development, Flow Cytometry -
MCP-1 antibody treatment enhances damage and impedes repair of the alveolar epithelium in influenza pneumonitis.
Authors: Narasaraju T, Ng HH, Phoon MC
Am. J. Respir. Cell Mol. Biol., 2009-07-17;42(6):732-43.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Platelet-derived growth factor-BB controls epithelial tumor phenotype by differential growth factor regulation in stromal cells.
Authors: Lederle W, Stark HJ, Skobe M, Fusenig NE, Mueller MM
Am. J. Pathol., 2006-11-01;169(5):1767-83.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
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