Mouse HB-EGF Antibody

Catalog #: MAB82391 Datasheet

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB82391-100
MAB82391-SP

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Mouse HB-EGF Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse HB-EGF in direct ELISAs.

Source

Recombinant Monoclonal Rat IgG_2A Clone # 891808R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived recombinant mouse HB-EGF
Asp63-Leu148
Accession # Q06186

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse HB‑EGF Monoclonal Antibody (Catalog # MAB8239).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse HB-EGF DuoSet ELISA Kit (Catalog # DY8239-05) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Mouse HB‑EGF ELISA Standard Curve. Recombinant Mouse HB-EGF protein was serially diluted 2-fold and captured by Rat Anti-Mouse HB-EGF Monoclonal Antibody ( Catalog # MAB8239) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse HB-EGF Monoclonal Antibody (Catalog # MAB82391) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HB-EGF

Heparin-binding EGF-like growth factor, HB-EGF, is a 19-23 kDa glycoprotein, member of the EGF family of mitogens that exists in multiple forms as a result of heterogenous O-glycosylation. HB-EGF is a growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. It is required for normal cardiac valve formation and normal heart function and promotes smooth muscle cell proliferation. HB-EGF may be involved in macrophage-mediated cellular proliferation. The cDNA for mouse Proheparin-binding EGF-like growth factor encodes a 208 amino acid residue transmembrane protein that is proteolytically cleaved to generate the soluble HB‑EGF. Like EGF, TGF-alpha, and AR, HB-EGF binds to the EGF receptor and activates the receptor tyrosine kinase. It has been suggested that the differential activities found for HB-EGF compared to EGF may be mediated by the heparin-binding properties of HB-EGF.

Long Name

Heparin Binding EGF-like Growth Factor

Entrez Gene IDs

1839 (Human); 15200 (Mouse)

Alternate Names

diphtheria toxin receptor (heparin-binding epidermal growth factor-like growthfactor); Dtr; DTRHEGFLdiphtheria toxin receptor (heparin-binding EGF-like growth factor); Dts; DTSF; HBEGF; HB-EGF; Hegfl; heparin-binding EGF-like growth factor; heparin-binding epidermal growth factor; proheparin-binding EGF-like growth factor

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