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Mouse Haptoglobin DuoSet ELISA

Catalog #: DY4409-05 Datasheet
Catalog # Availability Size / Price Qty
DY4409-05
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Mouse Haptoglobin ELISA Standard Curve
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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Mouse Haptoglobin DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
62.5 - 4,000 pg/mL
Sufficient Materials
For five 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse Haptoglobin. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A Mouse Haptoglobin ELISA Standard Curve View Larger

Mouse Haptoglobin ELISA Standard Curve

Product Datasheets

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Product Datasheet

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Haptoglobin

Haptoglobin is an acute phase glycoprotein that is an inactive member of the peptidase S1 family of serine proteases. It is secreted by multiple cell types and binds with high affinity to circulating Hemoglobin. Association of Haptoglobin with free Hemoglobin protects tissues from Hemoglobin-mediated oxidative damage and facilitates Hemoglobin clearance. In addition, Haptoglobin has anti-inflammatory properties that are independent of Hemoglobin binding. It inhibits prostaglandin synthesis, scavenges nitric oxide, dampens the inflammatory response to endotoxins, and inhibits T cell proliferation and neutrophil respiratory bursts. Deletion of Haptoglobin in mice facilitates the development of autoimmune inflammation.

Entrez Gene IDs:
3240 (Human); 15439 (Mouse); 24464 (Rat)
Alternate Names:
binding peptide; BP; haptoglobin alpha(1S)-beta; haptoglobin alpha(2FS)-beta; Haptoglobin; haptoglobin, alpha polypeptide; haptoglobin, beta polypeptide; HP; HP-1; hp2-alpha; HP2ALPHA2; HPA1S; MGC111141; zonulin

FAQs

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