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Mouse GM-CSF Antibody

Catalog #: AF-415-NA
6 Citations Datasheet
Catalog # Availability Size / Price Qty
AF-415-NA
AF-415-SP
Cell Proliferation Induced by GM‑CSF and Neutralization by Mouse GM‑CSF Antibody.
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Citations (6)
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Mouse GM-CSF Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse GM-CSF in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse GM-CSF
Ala18-Lys141
Accession # Q14AD9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse GM-CSF (Catalog # 415-ML)
Neutralization
Measured by its ability to neutralize GM‑CSF-induced proliferation in the DA3 mouse myeloma cell line. Ihle, J.N. et al. (1984) Advances in Viral Oncology. In G. Klein (eds): Raven Press, New York, NY. 4:95. The Neutralization Dose (ND50) is typically 0.01-0.03 µg/mL in the presence of 0.75 ng/mL Recombinant Mouse GM‑CSF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by GM‑CSF and Neutralization by Mouse GM‑CSF Antibody. View Larger

Cell Proliferation Induced by GM‑CSF and Neutralization by Mouse GM‑CSF Antibody. Recombinant Mouse GM-CSF (415-ML) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse GM-CSF (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse GM-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-415-NA). The ND50 is typically 0.01‑0.03 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GM-CSF

GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils. GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity. It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines. The 22 kDa glycosylated GM-CSF, similar to IL-3 and IL-5, is a cytokine with a core of four bundled alpha ‑helices. Mature mouse GM-CSF shares 49-54% amino acid sequence identity with canine, feline, human, and porcine GM-CSF and 69% with rat GM-CSF. GM‑CSF exerts its biological effects through a heterodimeric receptor complex composed of GM-CSF R alpha /CD116 and the signal transducing common beta chain (CD131) which is also a component of the high-affinity receptors for IL-3 and IL-5. In addition, GM-CSF binds a naturally occurring soluble form of GM-CSF R alpha. The activity of GM-CSF is species specific between human and mouse. Mouse GM-CSF is only weakly active on rat cells, although rat GM-CSF is fully active on mouse cells.

Long Name
Granulocyte Macrophage Growth Factor
Entrez Gene IDs
1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)
Alternate Names
colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; CSF2; CSF-2; GMCSF; GM-CSF; GMCSFgranulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897; Molgramostim; molgramostin; Sargramostim

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Citations for Mouse GM-CSF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Central Nervous System Barriers Impact Distribution and Expression of iNOS and Arginase-1 in Infiltrating Macrophages During Neuroinflammation
    Authors: Daniela C. Ivan, Sabrina Walthert, Giuseppe Locatelli
    Frontiers in Immunology
  2. Maltose-binding protein fusion allows for high level bacterial expression and purification of bioactive mammalian cytokine derivatives.
    Authors: Pennati A, Deng J, Galipeau J
    PLoS ONE, 2014-09-08;9(9):e106724.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. GM-CSF-licensed CD11b+ lung dendritic cells orchestrate Th2 immunity to Blomia tropicalis.
    Authors: Zhou Q, Ho A, Schlitzer A, Tang Y, Wong K, Wong F, Chua Y, Angeli V, Mortellaro A, Ginhoux F, Kemeny D
    J Immunol, 2014-06-18;193(2):496-509.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  4. A GMCSF and IL-15 fusokine leads to paradoxical immunosuppression in vivo via asymmetrical JAK/STAT signaling through the IL-15 receptor complex.
    Authors: Rafei M, Wu JH, Annabi B, Lejeune L, Francois M, Galipeau J
    Blood, 2006-11-02;109(5):2234-42.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  5. Recombinant Leishmania major secreting biologically active granulocyte-macrophage colony-stimulating factor survives poorly in macrophages in vitro and delays disease development in mice.
    Authors: Dumas C, Muyombwe A, Roy G, Matte C, Ouellette M, Olivier M, Papadopoulou B
    Infect. Immun., 2003-11-01;71(11):6499-509.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  6. A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells.
    Authors: Shih CC, Hu MC, Hu J
    Blood, 2000-03-15;95(6):1957-66.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization

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