Mouse GDF-15 Antibody Summary
Ser189-Ala303
Accession # Q9Z0J7
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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GDF‑15 in Mouse Brain. GDF-15 was detected in perfusion fixed frozen sections of mouse brain (trigeminal ganglion) using Sheep Anti-Mouse GDF-15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6385) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GDF-15
GDF-15 (Growth/differentiation factor 15; also PTGF, PDF, PL-74 and NAG-1) is a 25-30 kDa homodimeric member of the TGF-beta superfamily of proteins. In rodent, GDF-15 is expressed in a diverse population of activated cell types, including hepatocytes, macrophages, Schwann cells, cardiomyocytes, osteoblasts, adipocytes, and epithelium of the small intestine and mammary gland. Functionally, GDF-15 has multiple effects, some tissue specific, including the induction of ACRP30 secretion from fat, an attenuation of the adverse effects of cardiac hypertrophy, and serving as a trophic factor for motor and sensory neurons. Mouse GDF-15 is synthesized as a 273 amino acid (aa) proprecursor that contains a cleavable 26 kDa, 158 aa glycosylated prodomain (aa 31-188) and a 12-14 kDa, 115 aa mature region (aa 189-303). In human, certain cells are noted to secrete an uncleaved 40 kDa proprecursor that, as a disulfide-linked homodimer, would run at about 80 kDa in nonreducing SDS-PAGE. Over aa 189-303, mouse GDF-15 shares 97% and 67% aa identity with rat and human GDF-15, respectively.
Product Datasheets
Citations for Mouse GDF-15 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Deadenylase-dependent mRNA decay of GDF15 and FGF21 orchestrates food intake and energy expenditure
Authors: Sakie Katsumura, Nadeem Siddiqui, Michael Rock Goldsmith, Jaime H. Cheah, Teppei Fujikawa, Genki Minegishi et al.
Cell Metabolism
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Targeting AURKA in treatment of peritoneal tumor dissemination in gastrointestinal cancer
Authors: H Ozawa, H Imazeki, Y Ogiwara, H Kawakubo, K Fukuda, Y Kitagawa, C Kudo-Saito
Translational Oncology, 2021-12-10;16(0):101307.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Fc-GDF15 glyco-engineering and receptor binding affinity optimization for body weight regulation
Authors: E Fung, L Kang, D Sapashnik, S Benard, A Sievers, Y Liu, G Yan, J Zhou, L Rodriguez, W Ma, WR Stochaj, E LaVallie, L Wroblewska, K Kelleher, A Tam, O Bezy, D Breen, JR Chabot, T He, L Lin, Z Wu, L Mosyak
Scientific Reports, 2021-04-26;11(1):8921.
Species: Mouse
Sample Types: Serum
Applications: ELISA Capture
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