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Mouse Flt-3/Flk-2 Antibody

Catalog #: AF768
3 Citations Datasheet
Catalog # Availability Size / Price Qty
AF768
AF768-SP
Product Details
Citations (3)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Flt-3/Flk-2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Flt‑3/Flk‑2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human Flt-3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Flt‑3/Flk‑2
Asn28-Ser544
Accession # Q00342
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Flt‑3/Flk‑2 Fc Chimera (Catalog # 768-F3)
Flow Cytometry
0.25 µg/106 cells
M1 mouse myeloid leukemia cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Flt-3/Flk-2

The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1 (stem cell tyrosine kinase), is a member of the class III subfamily of receptor tyrosine kinases. This familay includes KIT, the receptor for SCF, and C-FMS, the receptor for M-CSF. The extracellular region of these receptors contains five immunoglobulin-like domains and the intracellular region contains a split kinase domain. Mouse Flt-3 cDNA encodes a 992 amino acid (aa) residue type I membrane protein with a 27 aa residue signal peptide, a 517 aa extracellular domain with 10 potential N-linked glycosylation sites, a 20 aa residue transmembrane domain and a 428 aa residue cytoplasmic domain. Mouse Flt-3 shares 85% amino acid sequence identity with human Flt-3. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin. Among hematopoietic cells, the expression of Flt-3 was found to be restricted to the highly enriched stem/progenitor cell populations. The ligand for Flt-3 (FL) has been identified to be a transmembrane protein with structural homology to M-CSF and SCF. Recombinant soluble Flt-3/Fc chimeric protein has been shown to bind FL with high affinity and is a potent FL antagonist.

References
  1. Rosnet, O. et al. (1996) Acta. Haemato. 95:218.
  2. Drexler, H.G. (1996) Leukemia 10:588.
Long Name
fms-like Tyrosine Kinase 3
Entrez Gene IDs
2322 (Human); 14255 (Mouse)
Alternate Names
CD135 antigen; CD135; EC 2.7.10; fetal liver kinase 2; FL cytokine receptor; FLK2; Flk-2; FLT3 receptor tyrosine kinase; Flt3; Flt-3; Fms-like tyrosine kinase 3; fms-related tyrosine kinase 3; growth factor receptor tyrosine kinase type III; Stem cell tyrosine kinase 1; STK-1; STK1EC 2.7.10.1; Tyrosine-protein kinase receptor FLT3

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Citations for Mouse Flt-3/Flk-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Disrupting PTPRJ transmembrane-mediated oligomerization counteracts oncogenic receptor tyrosine kinase FLT3 ITD
    Authors: Marie Schwarz, Sophie Rizzo, Walter Espinoza Paz, Anne Kresinsky, Damien Thévenin, Jörg P. Müller
    Frontiers in Oncology
  2. Features of Ras activation by a mislocalized oncogenic tyrosine kinase: FLT3 ITD signals through K-Ras at the plasma membrane of acute myeloid leukemia cells
    Authors: Susanne Köthe, Jörg P. Müller, Sylvia-Annette Böhmer, Todor Tschongov, Melanie Fricke, Sina Koch et al.
    Journal of Cell Science
  3. Protein-tyrosine phosphatase DEP-1 controls receptor tyrosine kinase FLT3 signaling.
    Authors: Arora D, Stopp S, Bohmer SA, Schons J, Godfrey R, Masson K, Razumovskaya E, Ronnstrand L, Tanzer S, Bauer R, Bohmer FD, Muller JP
    J. Biol. Chem., 2011-01-24;286(13):10918-29.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation

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