Mouse Fibroblast Activation Protein alpha /FAP Antibody
Mouse Fibroblast Activation Protein alpha /FAP Antibody Summary
Leu26-Asp761
Accession # P97321
Applications
This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse Fibroblast Activation Protein alpha /FAP Monoclonal Antibody (Catalog # MAB9727).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fibroblast Activation Protein alpha/FAP
FAP (also known as Seprase) is a 95 kDa Type II transmembrane serine protease that is structurally related to dipeptidyl peptidase IV (DPPIV/CD26) (1, 2). Within the extracellular domain, mouse FAP shares 90% and 97% amino acid (aa) sequence identity with human and rat FAP, respectively (3, 4). Alternative splicing of mouse FAP generates isoforms with a 33 aa or 5 aa deletion in the extracellular juxtamembrane region (3). FAP is expressed on reactive stromal fibroblasts in tumor tissue and wound healing and on synoviocytes in rheumatoid arthritis (1, 5-7). It exhibits dipeptidyl peptidase activity with substrate specificity similar to DPPIV, which is specific for N-terminal Xaa-Pro sequences (5, 8). FAP is also an endopeptidase that can degrade Gelatin, Collagens I and IV, Fibronectin, and Laminin (1, 5, 8) as well as several peptide hormones (e.g. Neuropeptide Y, Brain Natriuretic Peptide, Substance P, Peptide YY, and Incretins) (9). The enzymatic activity is dependent on FAP association with DPPIV on the cell surface (5, 8, 10, 11). The matrix-dedgrading activity of FAP contributes to tumor cell migration and invasion (10-13). In addition, FAP can enhance tumor cell growth by limiting the development of anti-tumor immunity (14).
- Zi, F. et al. (2015) Mol. Med. Rep. 11:3203.
- Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595.
- Niedermeyer, J. et al. (1997) Int. J. Cancer 71:383.
- Scanlan, M.J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5657.
- Park, J.E. et al. (1999) J. Biol. Chem. 274:36505.
- Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110.
- Bauer, S. et al. (2006) Arthritis Res. 8:R171.
- Aertgeerts, K. et al. (2005) J. Biol. Chem. 280:19441.
- Keane, F.M. et al. (2011) FEBS J. 278:1316.
- Ghersi, G. et al. (2006) Cancer Res. 66:4652.
- Ghersi, G. et al. (2002) J. Biol. Chem. 277:29231.
- Cheng, J.D. et al. (2005) Mol. Cancer Ther. 4:351.
- Cheng, J.D. et al. (2002) Cancer Res. 62:4767.
- Kraman, M. et al. (2010) Science 330:827.
Product Datasheets
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