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Mouse FGFR5/FGFRL1 Antibody

Catalog #: AF1899
2 Citations Datasheet
Catalog # Availability Size / Price Qty
AF1899
AF1899-SP
FGF R5/FGFRL1 in Mouse Lung.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse FGFR5/FGFRL1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse FGF R5 beta in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) FGF R1 alpha  (IIIb), recombinant mouse (rm) FGF R2 beta  (IIIc), rmFGF R3 alpha  (IIIb), rhFGF R3 alpha  (IIIc), rhFGF R1 beta  (IIIc), rmFGF R2 beta  (IIIb), rhFGF R1 alpha  (IIIc), rmFGF R2 alpha  (IIIc), rhFGF R1 beta  (IIIb), rmFGF R2 alpha (IIIb), rmFGF R2, and rmFGF R4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse FGF R5 beta
Ala21-Pro374
Accession # Q91V87
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse FGF R5/FGFRL1 Fc Chimera (Catalog # 1899-FR)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry FGF R5/FGFRL1 antibody in Mouse Lung by Immunohistochemistry (IHC-Fr). View Larger

FGF R5/FGFRL1 in Mouse Lung. FGF R5/FGFRL1 was detected in perfusion fixed frozen sections of mouse lung using Goat Anti-Mouse FGF R5/FGFRL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1899) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane and cytoplasm of alveolar cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGFR5/FGFRL1

Fibroblast growth factor receptor 5 (FGF R5), also known as FGRL1, is a 65 kDa transmembrane member of the FGF receptor family (1). Mature mouse FGF R5 consists of a 354 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 134 aa cytoplasmic domain (2). FGF R5 is distinct from other FGF receptor family members in that it does not contain a cytoplasmic tyrosine kinase domain (2, 3). Within the ECD, mouse FGF R5 shares 94% and 98% aa sequence identity with human and rat FGF R5, respectively. Alternate splicing results in an isoform that lacks the first Ig-like domain (2). FGF R5 is widely expressed, and expression of two species of mRNA in cartilage and pancreas may indicate the presence of both splice forms (2-6). Both the full length and truncated isoforms of FGF R5 bind FGF basic; the full length form additionally binds to heparin (2, 6). FGF R5 may function as a decoy receptor by binding FGF but not transducing its mitogenic signals (6).

References
  1. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  2. Sleeman, M. et al. (2001) Gene 271:171.
  3. Kim, I. et al. (2001) Biochim. Biophys. Acta 1518:152.
  4. Antoine, M. et al. (2005) Growth Factors 23:87.
  5. Wiedemann, M. et al. (2000) Genomics 69:275.
  6. Trueb, B. et al. (2003) J. Biol. Chem. 278:33857.
Long Name
Fibroblast Growth Factor Receptor-like 1
Entrez Gene IDs
53834 (Human); 116701 (Mouse)
Alternate Names
FGF homologous factor receptor; FGF R5; FGF receptor-like protein 1; FGFR5; FGFR-5; FGFRL1; FGFR-like protein; FHFR; Fibroblast growth factor receptor 5; fibroblast growth factor receptor-like 1

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Citations for Mouse FGFR5/FGFRL1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Targeted disruption of the intracellular domain of receptor FgfrL1 in mice.
    Authors: Bluteau G, Zhuang L, Amann R, Trueb B
    PLoS ONE, 2014-08-15;9(8):e105210.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. The murine Fgfrl1 receptor is essential for the development of the metanephric kidney.
    Authors: Gerber SD, Steinberg F, Beyeler M, Villiger PM, Trueb B
    Dev. Biol., 2009-08-26;335(1):106-19.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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