Mouse FGFR2 (IIIb) Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB7161N Datasheet
Catalog # Availability Size / Price Qty
FAB7161N-100UG
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Mouse FGFR2 (IIIb) Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects IIIb isoforms of mouse FGF R2 in direct ELISAs and Western blots. This antibody shows approximately 75% cross-reactivity with recombinant human (rh) FGF R2 (IIIb) isoforms and no cross-reactivity with the IIIc isoform of mouse or human FGF R2
Source
Monoclonal Rat IgG2a Clone # 133730
Purification
Protein A or G purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse FGF R2 isoforms and S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse FGF R2 isoforms
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: FGFR2

FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGF R1 through 5. FGF Rs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGF R1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGF R5 does not. Multiple forms of FGF R1, 2, and 3 are generated by alternative splicing

Long Name
Fibroblast Growth Factor Receptor 2
Entrez Gene IDs
2263 (Human); 14183 (Mouse)
Alternate Names
BBDS; BEK; BFR-1; CD332 antigen; CD332; CEK3; CFD1; craniofacial dysostosis 1; EC 2.7.10; EC 2.7.10.1; ECT1; FGF R2; FGFR2; fibroblast growth factor receptor 2; FLJ98662; Jackson-Weiss syndrome; JWS; Keratinocyte growth factor receptorreceptor like 14; KGFR; KSAM; K-sam; soluble FGFR4 variant 4; TK14; TK25

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