Mouse FGF acidic/FGF1 Antibody

Catalog #: AF4686
3 Citations 1 Review Datasheet

Catalog #	Availability	Size / Price	Qty
AF4686
AF4686-SP

Detection of Mouse FGF acidic/FGF1 by Western Blot.

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All FGF acidic/FGF1 Antibodies

Product Details

Citations (3)

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse FGF acidic/FGF1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse FGF acidic/FGF1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human FGF acidic is observed, and less than 1% cross-reactivity with recombinant mouse (rm) FGF7, rmFGF21, and rmFGF23 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse FGF acidic/FGF1
Phe16-Asp155
Accession # NP_034327

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Neutralization

Measured by its ability to neutralize FGF acidic/FGF1-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Raines, E.W. et al. (1985) Methods Enzymol. 109:749.The Neutralization Dose (ND₅₀) is typically 0.1-0.6 μg/mL in the presence of 1 ng/mL Recombinant Mouse FGF acidic/FGF1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse FGF acidic/FGF1 by Western Blot. Western blot shows lysates of mouse heart tissue and mouse kidney tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Mouse FGF acidic/FGF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4686) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FGF acidic/FGF1 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Neutralization

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Cell Proliferation Induced by FGF acidic/FGF1 and Neutralization by Mouse FGF acidic/FGF1 Antibody. Recombinant Mouse FGF acidic/FGF1 (Catalog # 4686-FA) induces proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse FGF acidic/FGF1 (1 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Mouse FGF acidic/FGF1 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF4686). The ND₅₀ is typically 0.1-0.6 µg/mL.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF acidic/FGF1

FGF acidic, also known as FGF1, ECGF, and HBGF-1, is a 17 kDa nonglycosylated member of the FGF family of mitogenic peptides. FGF acidic, which is produced by multiple cell types, stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis (1-3). Mouse FGF acidic shares 52% amino acid (aa) sequence identity with FGF basic and 15%-35% with other mouse FGFs. It shares 91%, 96%, 94%, and 100% aa sequence identity with bovine, human, porcine, and rat FGF acidic, respectively, and exhibits considerable species crossreactivity. During its nonclassical secretion, FGF acidic associates with S100A13, copper ions, and the C2A domain of synaptotagmin 1 (4). The secreted FGF acidic is stored in complex with extracellular heparan sulfate (5). The ability of heparan sulfate to bind FGF acidic is determined by its pattern of sulfation, and alterations in this pattern during embryogenesis thereby regulating FGF acidic bioactivity (6). The association of FGF acidic with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors (7, 8). Ligation triggers receptor dimerization, transphosphorylation, and internalization of receptor/FGF complexes (9). Internalized FGF acidic can translocate to the cytosol with the assistance of Hsp90 and then migrate to the nucleus by means of its two nuclear localization signals (10-12). The phosphorylation of FGF acidic by nuclear PKC delta triggers its active export to the cytosol where it is dephosphorylated and degraded (13, 14). Intracellular FGF acidic functions as a survival factor by inhibiting p53 activity and proapoptotic signaling (15).

References

Madiai, F. et al. (1996) Gene 179:231.
Galzie, Z. et al. (1997) Biochem. Cell Biol. 75:669.
Presta, M. et al. (2005) Cytokine Growth Factor Rev. 16:159.
Rajalingam, D. et al. (2007) Biochemistry 46:9225.
Guerrini, M. et al. (2007) Curr. Pharm. Des. 13:2045.
Allen, B.L. and A.C. Rapraeger (2003) J. Cell Biol. 163:637.
Robinson, C.J. et al. (2005) J. Biol. Chem. 280:42274.
Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
Wiedlocha, A. and V. Sorensen (2004) Curr. Top. Microbiol. Immunol. 286:45.
Wesche, J. et al. (2006) J. Biol. Chem. 281:11405.
Imamura, T. et al. (1990) Science 249:1567.
Wesche, J. et al. (2005) Biochemistry 44:6071.
Wiedlocha, A. et al. (2005) Mol. Biol. Cell 16:794.
Nilsen, T. et al. (2007) J. Biol. Chem. 282:26245.
Bouleau, S. et al. (2005) Oncogene 24:7839.

Long Name

Fibroblast Growth Factor acidic

Entrez Gene IDs

2246 (Human); 14164 (Mouse); 25317 (Rat); 281160 (Bovine)

Alternate Names

AFGF; alpha; alpha-ECGF; beta-ECGF; ECGF; ECGFB; ECGF-betaAcidic fibroblast growth factor; endothelial cell growth factor, beta; FGF acidic; FGF-1; FGFABeta-endothelial cell growth factor; FGF-alpha; fibroblast growth factor 1 (acidic); GLIO703; HBGF1; HBGF-1; heparin-binding growth factor 1

Product Datasheets

Product Datasheet

Related Research Areas

Citations for Mouse FGF acidic/FGF1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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FGF1 Signaling Modulates Biliary Injury and Liver Fibrosis in the Mdr2-/- Mouse Model of Primary Sclerosing Cholangitis
Authors: O'Brien A, Zhou T, White T et al.
Hepatology communications
Sensory nerve niche regulates mesenchymal stem cell homeostasis via FGF/mTOR/autophagy axis
Authors: F Pei, L Ma, J Jing, J Feng, Y Yuan, T Guo, X Han, TV Ho, J Lei, J He, M Zhang, JF Chen, Y Chai
Nature Communications, 2023-01-20;14(1):344.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Lack of miR-378 attenuates muscular dystrophy in mdx mice
Authors: P Podkalicka, O Mucha, I Bronisz-Bu, M Kozakowska, K Pietraszek, A Cetnarowsk, U G?owniak-K, K Bukowska-S, M Cie?la, M Kulecka, J Ostrowski, M Miku?a, A Potulska-C, A Kostera-Pr, A Józkowicz, A ?oboda, J Dulak
JCI Insight, 2020-06-04;5(11):.
Species: Transgenic Mouse
Sample Types: Cell Lysates
Applications: Western Blot