Mouse FGF-4 Antibody

Catalog #: MAB5846 Datasheet

Catalog #	Availability	Size / Price	Qty
MAB5846
MAB5846-SP

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Mouse FGF-4 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse FGF-4 in direct ELISAs. In direct ELISAs, 10% - 50% cross-reactivity with recombinant mouse (rm) FGF-10, rmFGF-7, recombinant human (rh) FGF-16, rhFGF-20 and less than 5% cross-reactivity with rmFGF acidic, rmFGF basic, rmFGF-6, -8b, -8c, -15, -17,
-21, -23, rhFGF acidic, rhFGF basic, rhFGF-3, -4, -5, -9, -11, -12, -13, -18, -19, or -22 is observed.

Source

Monoclonal Rat IgG_2B Clone # 678431

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse FGF-4
Ala30-Leu202
Accession # NP_034332

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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FGF‑4 in Mouse Embryo. FGF-4 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Rat Anti-Mouse FGF-4 Monoclonal Antibody (Catalog # MAB5846) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-4

FGF-4 (fibroblast growth factor-4), also known as FGF-K or K-FGF (Kaposi’s sarcoma-associated FGF), is a 25 kDa secreted, heparin-binding member of the FGF family (1, 2). The mouse FGF-4 cDNA encodes 202 amino acids (aa) with a 29 aa signal sequence and a 173 aa mature protein with an FGF homology domain that contains a heparin binding region near the C-terminus (2). Mature mouse FGF-4 shares 87%, 90%, 87% and 85% aa identity with human, rat, canine and bovine FGF‑4, respectively. Human FGF-4 has been shown to exhibit cross species activity. Expression of FGF-4 and its receptors, FGF R1c, 2c, 3c and 4, is spatially and temporally regulated during embryonic development (1, 3). Its expression in the trophoblast inner cell mass promotes expression of FGF R2, and is required for maintenance of the trophectoderm and primitive endoderm (3‑5). Later in development, FGF-4 works together with FGF-8 to mediate the activities of the apical ectodermal ridge, which direct the outgrowth and patterning of vertebrate limbs (3, 6‑9). FGF-4 is proposed to play a physiologically relevant role in human embryonic stem cell self-renewal. It promotes stem cell proliferation, but may also aid differentiation depending on context and concentration, and is often included in embryonic stem cell media in vitro (10‑12). A C-terminally truncated 15 kDa isoform that opposes full-length FGF-4 and promotes differentiation is endogenously expressed in human embryonic stem cells. FGF-4 is mitogenic for fibroblasts and endothelial cells in vitro and has autocrine transforming potential (13). It is a potent angiogenesis promoter in vivo and has been investigated as therapy for coronary artery disease (14).

References

Reuss, B. and O. von Bohlen und Halbach (2003) Cell Tiss. Res. 313:139.
Hebert, J.M. et al. (1990) Dev. Biol. 138:454.
Niswander, L. and G.R. Martin (1992) Development 114:755.
Feldman, B. et al. (1995) Science 267:246.
Goldin, S.N. and V.E. Papaioannou (2003) Genesis 36:40.
Sun, X. et al. (2002) Nature 418:501.
Boulet, A.M. et al. (2004) Dev. Biol. 273:361.
Yu, K and D.M. Ornitz (2008) Development 135:483.
Mariani, F.V. et al. (2008) Nature 453:401.
Johannesson, M. et al. (2009) PLoS ONE 4:e4794.
Kunath, T. et al. (2007) Development 134:2895.
Mayshar, Y. et al. (2008) Stem Cells 26:767.
Hajitou, A. et al. (1998) Oncogene 17:2059.
Flynn, A. and T. O’Brien (2008) IDrugs 11:283.

Long Name

Fibroblast Growth Factor 4

Entrez Gene IDs

2249 (Human); 14175 (Mouse); 116499 (Rat)

Alternate Names

FGF4; FGF-4; fibroblast growth factor 4; HBGF-4; HBGF-4Transforming protein KS3; heparin secretory transforming protein 1; Heparin secretory-transforming protein 1; Heparin-binding growth factor 4; HST-1; HST-1HSTF-1; HSTF1fibroblast growth factor 4 splice isoform; HSTFGF-4; human stomach cancer, transforming factor from FGF-related oncogene; kaposi sarcoma oncogene; KFGF; K-FGF; KS3; oncogene HST