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Mouse EphA2 Antibody

Catalog #: AF639
15 Citations Datasheet
Catalog # Availability Size / Price Qty
AF639
AF639-SP
Product Details
Citations (15)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse EphA2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse EphA2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) EphA2 is observed, and less than 1% cross‑reactivity with rhEphA1, recombinant mouse (rm) EphA3, rmEphA4, rmEphA6, rmEphA7, rmEphA8, and recombinant rat EphA5 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse EphA2
Ala22-Ala535
Accession # AAA82113
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse EphA2 Fc Chimera (Catalog # 639-A2)
Immunohistochemistry
5-15 µg/mL
Immersion fixed frozen sections of mouse embryo (E11)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EphA2

EphA2, also known as Eck, Myk2, and Sek2 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA2 has been shown to bind ephrin-A3, ephrin-A1, ephrin-A5, ephrin-A4, and ephrin-A2 (2, 3). The extracellular domains of mouse and human EphA2 share greater than 92% amino acid identity. Only membrane-bound or Fc‑clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

References
  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaegen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell. Biol. 9:608.
Entrez Gene IDs
1969 (Human); 13836 (Mouse)
Alternate Names
ARCC2; EC 2.7.10; EC 2.7.10.1; Eck; ECKepithelial cell receptor protein tyrosine kinase; EPH receptor A2; EphA2; ephrin type-A receptor 2; Epithelial cell kinase; Myk2; Sek2; soluble EPHA2 variant 1; Tyrosine-protein kinase receptor ECK

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Citations for Mouse EphA2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Epha2 and Efna5 participate in lens cell pattern-formation
    Authors: Yuefang Zhou, Alan Shiels
    Differentiation
  2. Formation of persistent hyperplastic primary vitreous in ephrin-A5-/- mice
    Authors: Alexander I. Son, Michal Sheleg, Margaret A. Cooper, Yuhai Sun, Norman J. Kleiman, Renping Zhou
    Investigative Opthalmology & Visual Science
  3. Genotype, Age, Genetic Background, and Sex Influence Epha2-Related Cataract Development in Mice
    Authors: A Dave, JE Craig, M Alamein, K Skrzypiec, J Beltz, A Pfaff, KP Burdon, N Ercal, RU de Iongh, S Sharma
    Investigative Ophthalmology & Visual Science, 2021-09-02;62(12):3.
    Species: Mouse
    Sample Types: WholeTissue
    Applications: IHC
  4. Brusatol-Mediated Inhibition of c-Myc Increases HIF-1? Degradation and Causes Cell Death in Colorectal Cancer under Hypoxia
    Authors: ET Oh, CW Kim, HG Kim, JS Lee, HJ Park
    Theranostics, 2017-08-11;7(14):3415-3431.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. EphA2 and ephrin-A5 are not a receptor-ligand pair in the ocular lens
    Authors: C Cheng, VM Fowler, X Gong
    Exp. Eye Res., 2017-06-23;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Ginsenoside Rg3 inhibition of vasculogenic mimicry in pancreatic cancer through downregulation of VEcadherin/EphA2/MMP9/MMP2 expression.
    Authors: Guo J, Zheng Q, Chen H, Chen L, Xu J, Chen M, Lu D, Wang Z, Tong H, Lin S
    Int J Oncol, 2014-06-16;45(3):1065-72.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Capillary defects and exaggerated inflammatory response in the airways of EphA2-deficient mice.
    Authors: Okazaki T, Ni A, Baluk P, Ayeni OA, Kearley J, Coyle AJ, Humbles A, McDonald DM
    Am. J. Pathol., 2009-05-14;174(6):2388-99.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  8. Bidirectional signaling through ephrinA2-EphA2 enhances osteoclastogenesis and suppresses osteoblastogenesis.
    Authors: Irie N, Takada Y, Watanabe Y, Matsuzaki Y, Naruse C, Asano M, Iwakura Y, Suda T, Matsuo K
    J. Biol. Chem., 2009-03-19;284(21):14637-44.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  9. Disruption of EphA2 receptor tyrosine kinase leads to increased susceptibility to carcinogenesis in mouse skin.
    Authors: Guo H, Miao H, Gerber L, Singh J, Denning MF, Gilliam AC, Wang B
    Cancer Res., 2006-07-15;66(14):7050-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  10. EphA2/Ephrin-A1 Signaling Complexes Restrict Corneal Epithelial Cell Migration
    Authors: Nihal Kaplan, Anees Fatima, Han Peng, Paul J. Bryar, Robert M. Lavker, Spiro Getsios
    Investigative Opthalmology & Visual Science
  11. Dlg-1 Interacts With and Regulates the Activities of Fibroblast Growth Factor Receptors and EphA2 in the Mouse Lens.
    Authors: Lee S, Shatadal S, Griep AE.
    Invest Ophthalmol Vis Sci
  12. A role for epha2 in cell migration and refractive organization of the ocular lens
    Authors: Yanrong Shi, Alicia De Maria, Thomas Bennett, Alan Shiels, Steven Bassnett
    Investigative Opthalmology & Visual Science
  13. Eph/Ephrin Promotes the Adhesion of Liver Tissue-Resident Macrophages to a Mimicked Surface of Liver Sinusoidal Endothelial Cells
    Authors: S Kohara, K Ogawa
    Biomedicines, 2022-12-12;10(12):.
  14. EphA2 promotes cell adhesion and spreading of monocyte and monocyte/macrophage cell lines on integrin ligand-coated surfaces
    Authors: Noritaka Saeki, Shingo Nishino, Tomohiro Shimizu, Kazushige Ogawa
    Cell Adhesion & Migration
  15. EphA2/Ephrin-A1 Mediate Corneal Epithelial Cell Compartmentalization via ADAM10 Regulation of EGFR Signaling
    Authors: Nihal Kaplan, Rosa Ventrella, Han Peng, Sonali Pal-Ghosh, Constadina Arvanitis, Joshua Z. Rappoport et al.
    Investigative Opthalmology & Visual Science

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