Mouse Endostatin Antibody

Catalog #: AF570
8 Citations 4 Reviews Datasheet

Catalog #	Availability	Size / Price	Qty
AF570
AF570-SP

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All Endostatin Antibodies

Product Details

Citations (8)

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Supplemental Products

Reviews (4)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Endostatin Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Endostatin in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human Endostatin is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Endostatin
His1105-Lys1288
Accession # AAA19787

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Endostatin

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Endostatin in Mouse Intestine. Endostatin was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse Endostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF570) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lacteals. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endostatin

Endostatin is a fragment of collagen XVIII and functions as an angiogenesis antagonist.

Entrez Gene IDs

80781 (Human); 12822 (Mouse)

Alternate Names

antiangiogenic agent; COL18A1; collagen alpha-1(XVIII) chain; collagen, type XVIII, alpha 1; Endostatin; FLJ27325; FLJ34914; human type XVIII collagen10endostatin; KNO; KNO1MGC74745; Knobloch syndrome, type 1; KS; multi-functional protein MFP

Product Datasheets

Product Datasheet

Related Research Areas

Citations for Mouse Endostatin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Type XVIII collagen is essential for survival during acute liver injury in mice
Authors: Michael B. Duncan, Changqing Yang, Harikrishna Tanjore, Patrick M. Boyle, Doruk Keskin, Hikaru Sugimoto et al.
Disease Models & Mechanisms
Ventilator-induced lung injury increases expression of endothelial inflammatory mediators in the kidney
Authors: Mark Hepokoski, Joshua A. Englert, Rebecca M. Baron, Laura E. Crotty-Alexander, Mark M. Fuster, Jeremy R. Beitler et al.
American Journal of Physiology-Renal Physiology
Combination therapy targeting integrins reduces glioblastoma tumor growth through antiangiogenic and direct antitumor activity and leads to activation of the pro-proliferative prolactin pathway.
Authors: Oliveira-Ferrer L, Wellbrock J, Bartsch U, Penas E, Hauschild J, Klokow M, Bokemeyer C, Fiedler W, Schuch G
Mol Cancer, 2013-11-20;12(1):144.
Species: Porcine
Sample Types: Whole Cells
Applications: Western Blot
Type IV collagen stimulates pancreatic cancer cell proliferation, migration, and inhibits apoptosis through an autocrine loop.
Authors: Ohlund D, Franklin O, Lundberg E, Lundin C, Sund M
BMC Cancer, 2013-03-26;13(0):154.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC-Fr
Host-derived MMP-13 exhibits a protective role in lung metastasis of melanoma cells by local endostatin production.
Authors: Fukuda H, Mochizuki S, Abe H, Okano HJ, Hara-Miyauchi C, Okano H, Yamaguchi N, Nakayama M, D'Armiento J, Okada Y
Br. J. Cancer, 2011-10-20;105(10):1615-24.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Cationic liposome coupled endostatin gene for treatment of peritoneal colon cancer.
Authors: Lan KL, Ou-Yang F, Yen SH, Shih HL, Lan KH
Clin. Exp. Metastasis, 2010-04-07;27(5):307-18.
Species: Mouse
Sample Types: Serum
Applications: ELISA Development
Endostatin peptide, an inhibitor of angiogenesis, prevents the progression of peritoneal sclerosis in a mouse experimental model.
Authors: Tanabe K, Maeshima Y, Ichinose K, Kitayama H, Takazawa Y, Hirokoshi K, Kinomura M, Sugiyama H, Makino H
Kidney Int., 2006-12-27;71(3):227-38.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Platelet-derived growth factor-BB controls epithelial tumor phenotype by differential growth factor regulation in stromal cells.
Authors: Lederle W, Stark HJ, Skobe M, Fusenig NE, Mueller MM
Am. J. Pathol., 2006-11-01;169(5):1767-83.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC