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Mouse EGF Antibody

Catalog #: AF2028
8 Citations 1 Review Datasheet
Catalog # Availability Size / Price Qty
AF2028
AF2028-SP
Cell Proliferation Induced by EGF and Neutralization by Mouse EGF Antibody.
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Product Details
Citations (8)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse EGF Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse EGF in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human EGF is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse EGF
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse EGF (Catalog # 2028-EG)
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize EGF-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 0.02-0.06 µg/mL in the presence of 0.3 ng/mL Recombinant Mouse EGF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by EGF and Neutralization by Mouse EGF Antibody. View Larger

Cell Proliferation Induced by EGF and Neutralization by Mouse EGF Antibody. Recombinant Mouse EGF (Catalog # 2028-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse EGF (0.3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2028). The ND50 is typically 0.02-0.06 µg/mL.

Immunohistochemistry EGF antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr). View Larger

EGF in Mouse Kidney. EGF was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2028) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGF

Epidermal growth factor (EGF) is the founding member of the EGF family that also includes TGF-alpha, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparin‑binding EGF‑like growth factor (HB-EGF), epigen, and the neuregulins (NRG)-1 through -6 (1). Members of the EGF family share a structural motif, the EGF‑like domain, which is characterized by three intramolecular disulfide bonds that are formed by six similarly spaced conserved cysteine residues (2). All EGF family members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The mature proteins are released from the cell surface by regulated proteolysis (1). The 1217 amino acid (aa) mouse EGF precursor contains nine EGF domains and nine LDLR class B repeats. The mature protein consists of 53 aa and is generated by proteolytic excision of the EGF domain proximal to the transmembrane region (3). Mature mouse EGF shares 70% and 77% aa sequence identity with mature human and rat EGF. EGF is present in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid (4). Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members (5). These receptors undergo a complex pattern of ligand induced homo- or hetero-dimerization to transduce EGF family signals (6, 7). EGF binds ErbB1 and depending on the context, induces the formation of homodimers or heterodimers containing ErbB2. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules (5, 8). Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.

References
  1. Harris, R.C. et al. (2003) Exp. Cell Res. 284:2.
  2. Carpenter, G. and Cohen, S. (1990) J. Biol. Chem. 265:7709.
  3. Gray, A. et al. (1983) Nature 303:722.
  4. Carpenter, G. and Zendegui, J.G. (1986) Exp. Cell Res. 164:1.
  5. Jorissen, R.N. et al. (2003) Exp. Cell Res. 284:31.
  6. Gamett, D.C. et al. (1997) J. Biol. Chem. 272:12052.
  7. Qian, X. et al. (1994) Proc. Natl. Acad. Sci. 91:1500.
  8. Qian, X. et al. (1999) J. Biol. Chem. 274:574.
Long Name
Epidermal Growth Factor
Entrez Gene IDs
1950 (Human); 13645 (Mouse); 25313 (Rat)
Alternate Names
beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; hEGF; HOMG4; pro-epidermal growth factor; URG; Urogastrone

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Citations for Mouse EGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Transcription factors AP-2alpha and AP-2beta regulate distinct segments of the distal nephron in the mammalian kidney
    Authors: JO Lamontagne, H Zhang, AM Zeid, K Strittmatt, AD Rocha, T Williams, S Zhang, AG Marneros
    Nature Communications, 2022-04-25;13(1):2226.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Magnesium and Calcium Homeostasis Depend on KCTD1 Function in the Distal Nephron
    Authors: AG Marneros
    Cell Reports, 2021-01-12;34(2):108616.
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. A critical role for NF2 and the Hippo pathway in branching morphogenesis
    Nat Commun, 2016-08-02;7(0):12309.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Neutralization
  4. VEGFR1-positive macrophages facilitate liver repair and sinusoidal reconstruction after hepatic ischemia/reperfusion injury.
    Authors: Ohkubo, Hirotoki, Ito, Yoshiya, Minamino, Tsutomu, Eshima, Koji, Kojo, Ken, Okizaki, Shin-ich, Hirata, Mitsuhir, Shibuya, Masabumi, Watanabe, Masahiko, Majima, Masataka
    PLoS ONE, 2014-08-27;9(8):e105533.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Leukotriene B4 type-1 receptor signaling promotes liver repair after hepatic ischemia/reperfusion injury through the enhancement of macrophage recruitment.
    Authors: Ohkubo, Hirotoki, Ito, Yoshiya, Minamino, Tsutomu, Mishima, Toshiaki, Hirata, Mitsuhir, Hosono, Kanako, Shibuya, Masabumi, Yokomizo, Takehiko, Shimizu, Takao, Watanabe, Masahiko, Majima, Masataka
    FASEB J, 2013-04-29;27(8):3132-43.
    Species: Mouse
    Sample Types: In Vivo, Whole Tissue
    Applications: IHC-Fr, Neutralization
  6. Tumor development in murine ulcerative colitis depends on MyD88 signaling of colonic F4/80+CD11b(high)Gr1(low) macrophages.
    Authors: Schiechl G, Bauer B, Fuss I, Lang SA, Moser C, Ruemmele P, Rose-John S, Neurath MF, Geissler EK, Schlitt HJ, Strober W, Fichtner-Feigl S
    J. Clin. Invest., 2011-04-25;121(5):1692-708.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  7. Tumoral NOX4 recruits M2 tumor-associated macrophages via ROS/PI3K signaling-dependent various cytokine production to promote NSCLC growth
    Authors: J Zhang, H Li, Q Wu, Y Chen, Y Deng, Z Yang, L Zhang, B Liu
    Redox Biol, 2019-02-06;22(0):101116.
  8. Opposing roles of CXCR4 and CXCR7 in breast cancer metastasis
    Authors: Lorena Hernandez, Marco A O Magalhaes, Salvatore J Coniglio, John S Condeelis, Jeffrey E Segall
    Breast Cancer Research

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Mouse EGF Antibody
By Anonymous on 05/08/2017
Application: WB Sample Tested: Recombinant protein Species: Mouse
Western Blot Mouse EGF Antibody AF2028