Mouse DPPIV/CD26 Biotinylated Antibody Summary
Ser29-His760
Accession # P28843
Applications
Mouse DPPIV/CD26 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of DPPIV/CD26 in Mouse Thymus. DPPIV/CD26 was detected in immersion fixed paraffin-embedded sections of mouse thymus using Goat Anti-Mouse DPPIV/CD26 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF954) at 10 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004) or the HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DPPIV/CD26
DPPIV/CD26 (EC 3.4.14.5) is a serine exopeptidase that releases Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides (1, 2). It is a type II membrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a long extracellular domain (3‑5). The extracellular domain contains glycosylation sites, a cysteine-rich region and the catalytic active site (Ser, Asp and His charge relay system). The amino acid sequence of the mouse DPPIV/CD26 extracellular domain is 84% and 91% identical to the human and rat counterparts, respectively. In the native state, DPPIV/CD26 is present as a noncovalently linked homodimer on the cell surface of a variety of cell types. The soluble form is also detectable in human serum and other body fluids, the levels of which may have clinical significance in patients with cancer, liver and kidney diseases, and depression.
DPPIV/CD26 plays an important role in many biological and pathological processes. It functions as T cell-activating molecule (THAM). It serves as a co‑factor for entry of HIV in CD4+ cells (6). It binds adenosine deaminase, the deficiency of which causes severe combined immunodeficiency disease in humans (7). It cleaves chemokines such as stromal-cell-derived factor 1 alpha and macrophage-derived chemokine (8, 9). It degrades peptide hormones such as glucagon (10). It truncates procalcitonin, a marker for systemic bacterial infections with elevated levels detected in patients with thermal injury, sepsis and severe infection, and in children with bacterial meningitis (11).
- Misumi, Y. and Y. Ikehara (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (eds), p. 1905, Elsevier, London.
- Ikehara, Y. et al. (1994) Methods Enzymol. 244:215.
- Marguet, D. et al. (1992) J. Biol. Chem. 267:2200.
- Bernard, A.M. et al. (1994) Biochemistry 33:15204.
- Vivier, I. et al. (1991) J. Immunol. 147:447.
- Callebaut, C. et al. (1993) Science 262:2045.
- Kameoka, J. et al. (1993) Science 261:466.
- Ohtsuki, T. et al. (1998) FEBS Lett. 431:236.
- Proost, P. et al. (1999) J. Biol. Chem. 274:3988.
- Hinke, S.A. et al. (2000) J. Biol. Chem. 275:3827.
- Wrenger, S. et al. (2000) FEBS Lett. 466:155.
Product Datasheets
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Mouse DPPIV/CD26 Biotinylated Antibody
There are currently no reviews for this product. Be the first to review Mouse DPPIV/CD26 Biotinylated Antibody and earn rewards!
Have you used Mouse DPPIV/CD26 Biotinylated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
