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Mouse DPPIV/CD26 Antibody

Catalog #: MAB9541
1 Citation Datasheet
Catalog # Availability Size / Price Qty
MAB9541
MAB9541-SP
Detection of DPPIV/CD26 in Mouse Splenocytes by Flow Cytometry.
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Mouse DPPIV/CD26 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse DPPIV/CD26 in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 155202
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse DPPIV/CD26
Extracellular domain
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of DPPIV/CD26 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of DPPIV/CD26 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse DPPIV/CD26 Monoclonal Antibody (Catalog # MAB9541, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DPPIV/CD26

DPPIV/CD26 (EC 3.4.14.5) is a serine exopeptidase that releases Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides (1, 2). It is a type II membrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a long extracellular domain (3‑5). The extracellular domain contains glycosylation sites, a cysteine-rich region and the catalytic active site (Ser, Asp and His charge relay system). The amino acid sequence of the mouse DPPIV/CD26 extracellular domain is 84% and 91% identical to the human and rat counterparts, respectively. In the native state, DPPIV/CD26 is present as a noncovalently linked homodimer on the cell surface of a variety of cell types. The soluble form is also detectable in human serum and other body fluids, the levels of which may have clinical significance in patients with cancer, liver and kidney diseases, and depression. DPPIV/CD26 plays an important role in many biological and pathological processes. It functions as T cell-activating molecule (THAM). It serves as a co‑factor for entry of HIV in CD4+ cells (6). It binds adenosine deaminase, the deficiency of which causes severe combined immunodeficiency disease in humans (7). It cleaves chemokines such as stromal-cell-derived factor 1 alpha and macrophage-derived chemokine (8, 9). It degrades peptide hormones such as glucagon (10). It truncates procalcitonin, a marker for systemic bacterial infections with elevated levels detected in patients with thermal injury, sepsis and severe infection, and in children with bacterial meningitis (11).

References
  1. Misumi, Y. and Y. Ikehara (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (eds), p. 1905, Elsevier, London.
  2. Ikehara, Y. et al. (1994) Methods Enzymol. 244:215.
  3. Marguet, D. et al. (1992) J. Biol. Chem. 267:2200.
  4. Bernard, A.M. et al. (1994) Biochemistry 33:15204.
  5. Vivier, I. et al. (1991) J. Immunol. 147:447.
  6. Callebaut, C. et al. (1993) Science 262:2045.
  7. Kameoka, J. et al. (1993) Science 261:466.
  8. Ohtsuki, T. et al. (1998) FEBS Lett. 431:236.
  9. Proost, P. et al. (1999) J. Biol. Chem. 274:3988.
  10. Hinke, S.A. et al. (2000) J. Biol. Chem. 275:3827.
  11. Wrenger, S. et al. (2000) FEBS Lett. 466:155.
Long Name
Dipeptidyl-peptidase IV
Entrez Gene IDs
1803 (Human); 13482 (Mouse); 397492 (Porcine); 102133935 (Cynomolgus Monkey)
Alternate Names
ADABP; ADCP-2; ADCP2DPP IV; Adenosine deaminase complexing protein 2TP103; CD26 antigen; CD26; CD26T-cell activation antigen CD26; dipeptidyl peptidase 4; Dipeptidyl peptidase IV; dipeptidylpeptidase 4; dipeptidyl-peptidase 4; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); DPP4; DPPIV; EC 3.4.14.5

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Citation for Mouse DPPIV/CD26 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Oxidized LDL upregulates macrophage DPP4 expression via TLR4/TRIF/CD36 pathways
    Authors: X Rao, S Zhao, Z Braunstein, H Mao, M Razavi, L Duan, Y Wei, AC Toomey, S Rajagopala, J Zhong
    EBioMedicine, 2019-02-07;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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