Mouse CXCL4/PF4 Antibody Summary
Val30-Ser105
Accession # Q9Z126
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse CXCL4/PF4 by Western Blot. Western blot shows lysate of mouse platelets. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse CXCL4/PF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF595) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CXCL4/PF4 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Mouse CXCL4/PF4 by Simple WesternTM. Simple Western lane view shows lysates of mouse platelets, loaded at 0.2 mg/mL. A specific band was detected for CXCL4/PF4 at approximately 14 kDa (as indicated) using 25 µg/mL of Goat Anti-Mouse CXCL4/PF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF595). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL4/PF4
CXCL4 is a member of the CXC chemokine family. Mouse CXCL4 is a 105 amino acid (aa) protein with a 29 aa signal sequence and a 76 aa mature protein. CXCL4 has homology with IL-8 and beta -thromboglobulin. Mouse and human CXCL4 share a 64% identity. Mouse and rat CXCL4 share 89% identity. CXCL4 contains several heparin-binding sites at the C-terminal region. The active protein consists of a tetramer composed of individual CXCL4 subunits. Megakaryocytes synthesize CXCL4 and store it as tetramers in alpha -granules. The CXCL4 tetramers are secreted by activated platelets and can be measured at micromolar levels in serum. In contrast to other CXC chemokines, CXCL4 lacks chemotactic activity for polymorphonuclear granulocytes. CXCL4 does not contain an ELR motif. However, many other fuctions have been observed for CXCL4. CXCL4 is involved in monocyte survivial and differentiation into macrophages, and it has anti-angiogenic activity. CXCL4 has been demonstrated to inhibit the binding of FGF-2 to high-affinity receptors and its subsequent internalization. Cell surface neutrophil chondroitin sulfate chains serve as CXCL4 binding sites; affinity is controlled by the degree of sulfation of these chains.
- Poncz, M. et al. (1987) Blood 69:219.
- Scheuerer, B. et al. (2000) Blood 95:1158.
- Perollet, C. et al. (1998) Blood 91:3289.
- Petersen, F. et al. (1998) J. Immunol. 161:4347.
- Petersen, F. et al. (1999) J. Biol. Chem. 274:12376.
- Watanabe, O. et al. (1999) J. Hum. Genet. 44:173.
Product Datasheets
Citations for Mouse CXCL4/PF4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Dual antiplatelet therapy with clopidogrel and aspirin increases mortality in 4T1 metastatic breast cancer-bearing mice by inducing vascular mimicry in primary tumour.
Authors: Smeda M, Kieronska A, et al.
Oncotarget
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Platelet factors attenuate inflammation and rescue cognition in ageing
Authors: Schroer, AB;Ventura, PB;Sucharov, J;Misra, R;Chui, MKK;Bieri, G;Horowitz, AM;Smith, LK;Encabo, K;Tenggara, I;Couthouis, J;Gross, JD;Chan, JM;Luke, A;Villeda, SA;
Nature
Species: Transgenic Mouse, Mouse
Sample Types: Platelets
Applications: Western Blot -
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
J. Clin. Invest., 2010-02-08;120(3):907-23.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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