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Mouse CXCL2/MIP-2 Antibody

Catalog #: AF-452-NA
29 Citations Datasheet
Catalog # Availability Size / Price Qty
AF-452-NA
AF-452-SP
Chemotaxis Induced by CXCL2/MIP-2 and Neutral-ization by Mouse CXCL2/ MIP-2 Antibody.
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Citations (29)
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Mouse CXCL2/MIP-2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CXCL2/MIP-2 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) GRO beta, recombinant mouse (rm) GRO gamma, rmKC and rmLIX is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CXCL2/MIP-2
Ala28-Asn100
Accession # P10889
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse thymus and immersion fixed paraffin-embedded sections of mouse thymus.
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize CXCL2/MIP-2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.015-0.075 µg/mL in the presence of 2 ng/mL Recombinant Mouse CXCL2/MIP-2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CXCL2/MIP-2 and Neutral-ization by Mouse CXCL2/ MIP-2 Antibody. View Larger

Chemotaxis Induced by CXCL2/MIP-2 and Neutral-ization by Mouse CXCL2/ MIP-2 Antibody. Recombinant Mouse CXCL2/MIP-2 (452-M2) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL2/MIP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-452-NA). The ND50 is typically 0.015‑0.075 µg/mL.

Immunocytochemistry CXCL2/MIP-2 antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

CXCL2/MIP‑2 in Mouse Splenocytes. CXCL2/MIP-2 was detected in immersion fixed mouse splenocytes stimulated with LPS and monensin using Goat Anti-Mouse CXCL2/MIP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-452-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry View Larger

Detection of CXCL2/GRO beta /MIP‑2/CINC‑3 in Mouse Thymus CXCL2/GRO beta /MIP‑2/CINC‑3 was detected in immersion fixed paraffin-embedded sections of mouse thymus using Goat Anti-Mouse CXCL2/GRO beta /MIP‑2/CINC‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-452-NA) at 15 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry Detection of Mouse CXCL2/GRO beta/MIP-2/CINC-3 by Immunohistochemistry View Larger

Detection of Mouse CXCL2/GRO beta/MIP-2/CINC-3 by Immunohistochemistry CXCL2 is produced by CD45+ cells in the brainstem infiltrates during aEAE. a Brainstem tissue was harvested from an IFN gamma RKO host at aEAE onset (clinical score 1). Sections were subjected to in situ hybridization to detect CXCL2 mRNA (red) combined with a DAPI (blue) counter stain. b Brainstem sections from a mouse with aEAE (clinical score 3) were stained with monoclonal antibodies specific for CD45 (green), GFAP (white), and CXCL2 (red) and counterstained with DAPI (blue). Arrows indicate CXCL2+ CD45+ cells. Arrow heads indicate GFAP+ astrocytes. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30012158), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse CXCL2/GRO beta/MIP-2/CINC-3 by Immunohistochemistry View Larger

Detection of Mouse CXCL2/GRO beta/MIP-2/CINC-3 by Immunohistochemistry CXCL2 is produced by CD45+ cells in the brainstem infiltrates during aEAE. a Brainstem tissue was harvested from an IFN gamma RKO host at aEAE onset (clinical score 1). Sections were subjected to in situ hybridization to detect CXCL2 mRNA (red) combined with a DAPI (blue) counter stain. b Brainstem sections from a mouse with aEAE (clinical score 3) were stained with monoclonal antibodies specific for CD45 (green), GFAP (white), and CXCL2 (red) and counterstained with DAPI (blue). Arrows indicate CXCL2+ CD45+ cells. Arrow heads indicate GFAP+ astrocytes Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30012158), licensed under a CC-BY license. Not internally tested by R&D Systems.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL2/GRO beta/MIP-2/CINC-3

Macrophage Inflammatory Protein-2 (MIP-2) was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. Based on its protein and DNA sequences, MIP-2 is a member of the alpha (C-X-C) subfamily of chemokines.

MIP-2 cDNA encodes a 100 amino acid residue precursor protein from which the amino-terminal 27 amino acid residues are cleaved to generate the mature MIP-2. The protein sequence of murine MIP-2 shows approximately 63% identity to that of murine KC, another murine alpha chemokine whose expression is induced by PDGF. In addition, the protein sequence of MIP-2 is also 60% identical to human GRO beta and GRO gamma. It has been suggested that mouse KC and MIP-2 are the homologs of the human GROs and rat CINCs.

Similarly to other alpha chemokines, murine MIP-2 is a potent neutrophil attractant and activator. MIP-2 and KC can bind the murine interleukin 8 type B receptor homologue with high affinity. The expression of MIP-2 was found to be associated with neutrophil influx in pulmonary inflammation and glomerulonephritis, suggesting that MIP-2 may contribute to the pathogenesis of inflammatory diseases.

Entrez Gene IDs
2920 (Human); 20310 (Mouse); 114105 (Rat)
Alternate Names
chemokine (C-X-C motif) ligand 2; CINC-2a; CINC3; CINC-3; C-X-C Motif Chemokine 2; CXCL2; GRO beta; GRO2 oncogene; GRO2; GROB; Gro-beta; Growth-regulated protein beta; Macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta; MGSA beta; MGSA-b; MGSA-beta; MIP2; MIP-2; MIP2A; MIP-2a; MIP2-alpha; SCYB2

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Citations for Mouse CXCL2/MIP-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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  1. Phospholipase C epsilon, an Effector of Ras and Rap Small GTPases, Is Required for Airway Inflammatory Response in a Mouse Model of Bronchial Asthma
    Authors: Tatsuya Nagano, Hironori Edamatsu, Kazuyuki Kobayashi, Nobuyuki Takenaka, Masatsugu Yamamoto, Naoto Sasaki et al.
    PLoS ONE
  2. Cryptotanshinone enhances wound healing in type 2 diabetes with modulatory effects on inflammation, angiogenesis and extracellular matrix remodelling
    Authors: Min Song, Lu Chen, Lusha Zhang, Chunxiao Li, Joel Wake Coffie, Zhirui Fang et al.
    Pharmaceutical Biology
  3. CXCL1 and CXCL2 Regulate NLRP3 Inflammasome Activation via G-Protein–Coupled Receptor CXCR2
    Authors: Monoranjan Boro, Kithiganahalli Narayanaswamy Balaji
    The Journal of Immunology
  4. Regulation of p53-mediated changes in the uPA-fibrinolytic system and in lung injury by loss of surfactant protein C expression in alveolar epithelial cells
    Authors: Bijesh Puthusseri, Amarnath Marudamuthu, Nivedita Tiwari, Jian Fu, Steven Idell, Sreerama Shetty
    American Journal of Physiology-Lung Cellular and Molecular Physiology
  5. Alveolar epithelial cells mitigate neutrophilic inflammation in lung injury through regulating mitochondrial fatty acid oxidation
    Authors: Chung, KP;Cheng, CN;Chen, YJ;Hsu, CL;Huang, YL;Hsieh, MS;Kuo, HC;Lin, YT;Juan, YH;Nakahira, K;Chen, YF;Liu, WL;Ruan, SY;Chien, JY;Plataki, M;Cloonan, SM;Carmeliet, P;Choi, AMK;Kuo, CH;Yu, CJ;
    Nature communications
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  6. IL-3 orchestrates ulcerative colitis pathogenesis by controlling the development and the recruitment of splenic reservoir neutrophils
    Authors: Bénard, A;Mittelstädt, A;Klösch, B;Glanz, K;Müller, J;Schoen, J;Nüse, B;Brunner, M;Naschberger, E;Stürzl, M;Mattner, J;Muñoz, LE;Sohn, K;Grützmann, R;Weber, GF;
    Cell reports
    Species: Mouse
    Sample Types:
    Applications: In Vivo
  7. Metabolic lactate production coordinates vasculature development and progenitor behavior in the developing mouse neocortex
    Authors: X Dong, Q Zhang, X Yu, D Wang, J Ma, J Ma, SH Shi
    Nature Neuroscience, 2022-06-20;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. C-X-C Chemokines Influence Intraocular Inflammation During Bacillus Endophthalmitis
    Authors: MH Mursalin, PS Coburn, FC Miller, ET Livingston, R Astley, MC Callegan
    Investigative Ophthalmology & Visual Science, 2021-11-01;62(14):14.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  9. Tumor-Associated Microglia/Macrophages as a Predictor for Survival in Glioblastoma and Temozolomide-Induced Changes in CXCR2 Signaling with New Resistance Overcoming Strategy by Combination Therapy
    Authors: RM Urbantat, C Jelgersma, S Brandenbur, M Nieminen-K, I Kremenetsk, J Zollfrank, S Mueller, K Rubarth, A Koch, P Vajkoczy, G Acker
    International Journal of Molecular Sciences, 2021-10-16;22(20):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Role of Innate Inflammation in the Regulation of Tissue Remodeling during Tooth Eruption
    Authors: Y Makino, K Fujikawa, M Matsuki-Fu, S Inoue, M Nakamura
    Dentistry journal, 2021-01-12;9(1):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Lack of whey acidic protein four disulphide core (WFDC) 2 protease inhibitor causes neonatal death from respiratory failure in mice
    Authors: K Nakajima, M Ono, U Radovi?, S Dizdarevi?, SI Tomizawa, K Kuroha, G Naganatsu, I Hoshi, R Matsunaga, T Shirakawa, T Kurosawa, Y Miyazaki, M Seki, Y Suzuki, H Koseki, M Nakamura, T Suda, K Ohbo
    Dis Model Mech, 2019-11-12;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Mannan-binding lectin deficiency exacerbates sterile liver injury in mice through enhancing hepatic neutrophil recruitment
    Authors: J Zhou, J Li, Y Yu, Y Liu, H Li, Y Liu, J Wang, L Zhang, X Lu, Z Chen, D Zuo
    J. Leukoc. Biol., 2018-10-23;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates, In Vivo
    Applications: Neutralization, Western Blot
  13. An IFN?/CXCL2 regulatory pathway determines lesion localization during EAE
    Authors: JS Stoolman, PC Duncker, AK Huber, DA Giles, JM Washnock-S, AM Soulika, BM Segal
    J Neuroinflammation, 2018-07-16;15(1):208.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Novel adherent CD11b+Gr-1+tumor-infiltrating cells initiate an immunosuppressive tumor microenvironment
    Authors: T Tsubaki, T Kadonosono, S Sakurai, T Shiozawa, T Goto, S Sakai, T Kuchimaru, T Sakamoto, H Watanabe, G Kondoh, S Kizaka-Kon
    Oncotarget, 2018-01-29;9(13):11209-11226.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. TIARP attenuates autoantibody-mediated arthritis via the suppression of neutrophil migration by reducing CXCL2/CXCR2 and IL-6 expression
    Sci Rep, 2016-12-20;6(0):38684.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Mycobacterium tuberculosis-triggered Hippo pathway orchestrates CXCL1/2 expression to modulate host immune responses
    Sci Rep, 2016-11-24;6(0):37695.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Neutralization
  17. The HMGB1/RAGE axis triggers neutrophil-mediated injury amplification following necrosis.
    Authors: Huebener, Peter, Pradere, Jean-Phi, Hernandez, Celine, Gwak, Geum-You, Caviglia, Jorge Ma, Mu, Xueru, Loike, John D, Jenkins, Rosalind, Antoine, Daniel J, Schwabe, Robert F
    J Clin Invest, 2014-12-22;125(2):539-50.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  18. Sphingolipid pathway regulates innate immune responses at the fetomaternal interface during pregnancy.
    Authors: Mizugishi K, Inoue T, Hatayama H, Bielawski J, Pierce J, Sato Y, Takaori-Kondo A, Konishi I, Yamashita K
    J Biol Chem, 2014-12-11;290(4):2053-68.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  19. Type I IFN suppresses Cxcr2 driven neutrophil recruitment into the sensory ganglia during viral infection.
    Authors: Stock A, Smith J, Carbone F
    J Exp Med, 2014-04-21;211(5):751-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  20. Neutrophil dependence of vascular remodeling after Mycoplasma infection of mouse airways.
    Authors: Baluk P, Phillips K, Yao L, Adams A, Nitschke M, McDonald D
    Am J Pathol, 2014-04-13;184(6):1877-89.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. CXCR2-mediated tumor-associated neutrophil recruitment is regulated by IFN-beta.
    Authors: Jablonska J, Wu C, Andzinski L, Leschner S, Weiss S
    Int J Cancer, 2013-10-31;134(6):1346-58.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC-Fr
  22. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010-02-08;120(3):907-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Initial delay in the immune response to Francisella tularensis is followed by hypercytokinemia characteristic of severe sepsis and correlating with upregulation and release of damage-associated molecular patterns.
    Authors: Mares CA, Ojeda SS, Morris EG, Li Q, Teale JM
    Infect. Immun., 2008-04-14;76(7):3001-10.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Functional Assay, IHC-Fr
  24. The Duffy antigen receptor for chemokines in acute renal failure: A facilitator of renal chemokine presentation.
    Authors: Zarbock A, Schmolke M, Bockhorn SG, Scharte M, Buschmann K, Ley K, Singbartl K
    Crit. Care Med., 2007-09-01;35(9):2156-63.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  25. CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response.
    Authors: Cardona AE, Gonzalez PA, Teale JM
    Infect. Immun., 2003-05-01;71(5):2634-42.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  26. The chemokine KC, but not monocyte chemoattractant protein-1, triggers monocyte arrest on early atherosclerotic endothelium.
    Authors: Huo Y, Weber C, Forlow SB, Sperandio M, Thatte J, Mack M, Jung S, Littman DR, Ley K
    J. Clin. Invest., 2001-11-01;108(9):1307-14.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-P
  27. Distinct Compartmentalization of the Chemokines CXCL1 and CXCL2 and the Atypical Receptor ACKR1 Determine Discrete Stages of Neutrophil Diapedesis
    Authors: Tamara Girbl, Tchern Lenn, Lorena Perez, Loïc Rolas, Anna Barkaway, Aude Thiriot et al.
    Immunity
  28. Tumor stem-like cell-derived exosomal RNAs prime neutrophils for facilitating tumorigenesis of colon cancer
    Authors: WL Hwang, HY Lan, WC Cheng, SC Huang, MH Yang
    J Hematol Oncol, 2019-01-25;12(1):10.
  29. Cellular content plays a crucial role in Non-typeable Haemophilus influenzae infection of preinflamed Junbo mouse middle ear
    Authors: Pratik P. Vikhe, Tom Purnell, Steve D.M. Brown, Derek W. Hood
    Cellular Microbiology

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