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Mouse CXCL1/GRO alpha /KC/CINC-1 Biotinylated Antibody

Catalog #: BAF453
23 Citations 2 Reviews Datasheet
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BAF453
Product Details
Citations (23)
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Reviews (2)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CXCL1/GRO alpha /KC/CINC-1 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CXCL1/GRO alpha /KC/CINC-1 in ELISAs and Western blots. In sandwich immunoassays, less than 40% cross-reactivity with recombinant rat (rr) CINC-1 is observed and less than 0.05% cross-reactivity with rrCINC-2 alpha, rrCINC-2 beta, recombinant mouse (rm) CRG-2, rmMIG, and rmMIP-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CXCL1/KC
Arg20-Lys96
Accession # P12850
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CXCL1/GRO alpha /KC/CINC‑1 aa 20-96 (Catalog # 453-KC)

Mouse CXCL1/KC Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse CXCL1/GRO alpha /KC/CINC‑1 Antibody (Catalog # MAB453R)

Capture Reagent: Mouse CXCL1/GRO alpha /KC/CINC‑1 Antibody (Catalog # MAB453)

Standard: Recombinant Mouse CXCL1/KC Protein (Catalog # 453-KC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL1/GRO alpha/KC/CINC-1

KC, a member of the alpha (CXC) chemokine subfamily, was initially identified as an immediate early gene induced in mouse fibroblasts by platelet-derived growth factor. KC cDNA encodes a 96 amino acid (aa) residue precursor protein with a predicted secretory signal peptide that is removed to yield the mature protein. The protein sequence of mouse KC shows approximately 63% identity to that of mouse MIP-2. KC is also approximately 60% identical to the human GROs. It has been suggested that mouse KC and MIP-2 are the orthologs of the human GROs and rat CINCs. In addition to mouse fibroblasts, KC is expressed in macrophages and endothelial cells. Mouse KC is a potent neutrophil attractant and activator. The functional receptor for KC has been identified as CXCR2. Based on the pattern of KC expression in a number of inflammatory disease models, KC appears to have an important role in inflammation. KC was found to be involved in monocyte arrest on atherosclerotic endothelium and may also play a pathophysiological role in Alzheimer’s disease. Many chemokines are substrates for selective proteolysis at the amino-terminus by various proteases including dipeptidyl peptidase IV or matrix metalloproteases, resulting in truncated chemokine isoforms with different (both enhanced or reduced) bioactivities. The naturally occurring 68 aa N-terminal truncated isoform of mouse KC is reported to be a more potent synergistic growth stimulants for CFU-GM. As a chemoattractant for hCXCR2 transfected mouse Baf/3 cells, the truncated form of mouse KC (aa 29-96) is also approximately 5-fold more active than 77 aa residue form of KC (aa 20-96, R&D Systems, Catalog # 453-KC) (1-4).

References
  1. King, A.G. et al. (2000) J. Immunol. 164:3774.
  2. Oquendo, P. et al. (1989) J. Biol. Chem. 264:4133.
  3. Huo, Y. et al. (2001) J. Clin. Invest. 108:1307.
  4. Xia, M. et al. (2002) J. Neuroimmunol. 122:55.
Entrez Gene IDs
2919 (Human); 14825 (Mouse); 81503 (Rat)
Alternate Names
CINC1; CINC-1; CXCL1; FSP; GRO alpha; GRO1; GROa; KC; MGSA; MGSA-a; MGSA-alpha; NAP-3; SCYB1

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Citations for Mouse CXCL1/GRO alpha /KC/CINC-1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. Differential binding of chemokines CXCL1, CXCL2 and CCL2 to mouse glomerular endothelial cells reveals specificity for distinct heparan sulfate domains
    Authors: JJ van Gemst, M Kouwenberg, ALWMM Rops, TH van Kuppev, JH Berden, TJ Rabelink, MA Loeven, J van der Vl
    PLoS ONE, 2018-09-24;13(9):e0201560.
    Species: Mouse
    Sample Types: Whole Cells
  2. Nlrp12 Mediates Adverse Neutrophil Recruitment during Influenza Virus Infection
    Authors: EE Hornick, B Banoth, AM Miller, ZR Zacharias, N Jain, ME Wilson, KN Gibson-Cor, KL Legge, GA Bishop, FS Sutterwala, SL Cassel
    J. Immunol., 2017-12-27;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
    Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
    Cell. Physiol. Biochem., 2017-08-09;42(5):1961-1972.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development (Detection)
  4. Malondialdehyde epitopes are sterile mediators of hepatic inflammation in hypercholesterolemic mice
    Authors: Clara Jana-Lui Busch
    Hepatology, 2017-02-03;0(0):.
    Species: Mouse
    Sample Types: Peritoneal Lavage Fluid
    Applications: ELISA Development (Detection)
  5. Nlrp12 mutation causes C57BL/6J strain-specific defect in neutrophil recruitment
    Authors: Tyler K. Ulland, Nidhi Jain, Emma E. Hornick, Eric I. Elliott, Gwendolyn M. Clay, Jeffrey J. Sadler et al.
    Nature Communications
  6. Pulmonary Epithelial Cell-Derived Cytokine TGF-beta1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function.
    Authors: Denney L, Byrne A, Shea T, Buckley J, Pease J, Herledan G, Walker S, Gregory L, Lloyd C
    Immunity, 2015-11-17;43(5):945-58.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Detection)
  7. MicroRNA-146a provides feedback regulation of lyme arthritis but not carditis during infection with Borrelia burgdorferi.
    Authors: Lochhead R, Ma Y, Zachary J, Baltimore D, Zhao J, Weis J, O'Connell R, Weis J
    PLoS Pathog, 2014-06-26;10(6):e1004212.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development
  8. Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections.
    Authors: Junkins R, MacNeil A, Wu Z, McCormick C, Lin T
    J Immunol, 2013-04-15;190(10):5178-86.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development (Detection)
  9. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012-01-25;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Development
  10. Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines.
    Authors: Kim J, Natarajan S, Vaickus LJ, Bouchard JC, Beal D, Cruikshank WW, Remick DG
    Am. J. Pathol., 2011-10-01;179(6):2730-9.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development
  11. Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
    Authors: Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ
    Proc. Natl. Acad. Sci. U.S.A., 2009-03-16;106(14):5954-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  12. ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T
    Microbes Infect., 2009-03-13;11(4):524-30.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  13. CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T
    Immunology, 2008-04-03;125(2):272-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  14. TNF-alpha and IL-1beta mediate inflammatory hypernociception in mice triggered by B1 but not B2 kinin receptor.
    Authors: Cunha TM, Verri WA, Jr, Fukada SY, Guerrero AT, Santodomingo-Garzon T, Poole S, Parada CA, Ferreira SH, Cunha FQ
    Eur. J. Pharmacol., 2007-07-13;573(1):221-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  15. Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis.
    Authors: Watanabe H, Kubo M, Numata K, Takagi K, Mizuta H, Okada S, Ito T, Matsukawa A
    J. Immunol., 2006-12-15;177(12):8650-7.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  16. Antiinflammatory effects of natural tetranortriterpenoids isolated from Carapa guianensis Aublet on zymosan-induced arthritis in mice.
    Authors: Penido C, Conte FP, Chagas MS, Rodrigues CA, Pereira JF, Henriques MG
    Inflamm. Res., 2006-11-01;55(11):457-64.
    Species: Mouse
    Sample Types: Synovial Fluid
    Applications: ELISA Development
  17. Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors.
    Authors: Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjostedt A, Edebro H, Forsman M, Bystrom M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK
    Infect. Immun., 2006-09-18;74(12):6730-8.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development
  18. Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4).
    Authors: Ramos CD, Fernandes KS, Canetti C, Teixeira MM, Silva JS, Cunha FQ
    Eur. J. Immunol., 2006-08-01;36(8):2025-34.
    Species: Mouse
    Sample Types: Tissue Secretion
    Applications: ELISA Development
  19. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
    Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
    J. Immunol., 2006-08-01;177(3):1967-74.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  20. Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury.
    Authors: Gao H, Hoesel LM, Guo RF, Rancilio NJ, Sarma JV, Ward PA
    J. Immunol., 2006-07-01;177(1):612-20.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development
  21. Sequential ELISA to profile multiple cytokines from small volumes.
    Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
    J. Immunol. Methods, 2005-07-01;302(1):172-81.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  22. Selective macrophage suppression during sepsis.
    Authors: Ellaban E, Bolgos G, Remick D
    Cell. Immunol., 2005-02-26;231(1):103-11.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  23. Regulatory effects of eotaxin on acute lung inflammatory injury.
    Authors: Guo RF, Lentsch AB, Warner RL, Huber-Lang M, Sarma JV, Hlaing T, Shi MM, Lukacs NW, Ward PA
    J. Immunol., 2001-04-15;166(8):5208-18.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development

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Mouse CXCL1/GRO alpha /KC/CINC-1 Biotinylated Antibody
By Katie Myers on 11/29/2022
Application: ELISA Sample Tested: Serum Species: Mouse
ELISA Mouse CXCL1/GRO alpha /KC/CINC-1 Biotinylated Antibody BAF453
Mouse CXCL1/GRO alpha /KC/CINC-1 Biotinylated Antibody
By Anonymous on 01/05/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Mouse
ELISA Mouse CXCL1/GRO alpha /KC/CINC-1 Biotinylated Antibody BAF453