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Mouse CXCL1/GRO alpha /KC/CINC-1 Antibody

Catalog #: AF-453-NA
32 Citations Datasheet
Catalog # Availability Size / Price Qty
AF-453-NA
AF-453-SP
Chemotaxis Induced by CXCL1/KC and Neutralization by Mouse CXCL1/KC Antibody.
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Citations (32)
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Mouse CXCL1/GRO alpha /KC/CINC-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CXCL1/GRO alpha /KC/CINC-1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CXCL1/GRO alpha /KC/CINC-1
Arg20-Lys96
Accession # P12850
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CXCL1/GRO alpha /KC/CINC‑1 aa 20-96 (Catalog # 453-KC)
Neutralization
Measured by its ability to neutralize CXCL1/GRO alpha /KC/CINC‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.3-1.5 µg/mL in the presence of 30 ng/mL Recombinant Mouse CXCL1/GRO alpha /KC/CINC‑1 aa 20‑96.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CXCL1/KC and Neutralization by Mouse CXCL1/KC Antibody. View Larger

Chemotaxis Induced by CXCL1/KC and Neutralization by Mouse CXCL1/KC Antibody. Recombinant Mouse CXCL1/KC (453-KC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/KC (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL1/KC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-453-NA). The ND50 is typically 0.3-1.5 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL1/GRO alpha/KC/CINC-1

KC, a member of the alpha (CXC) chemokine subfamily, was initially identified as an immediate early gene induced in mouse fibroblasts by platelet-derived growth factor. KC cDNA encodes a 96 amino acid (aa) residue precursor protein with a predicted secretory signal peptide that is removed to yield the mature protein. The protein sequence of mouse KC shows approximately 63% identity to that of mouse MIP-2. KC is also approximately 60% identical to the human GROs. It has been suggested that mouse KC and MIP-2 are the orthologs of the human GROs and rat CINCs. In addition to mouse fibroblasts, KC is expressed in macrophages and endothelial cells. Mouse KC is a potent neutrophil attractant and activator. The functional receptor for KC has been identified as CXCR2. Based on the pattern of KC expression in a number of inflammatory disease models, KC appears to have an important role in inflammation. KC was found to be involved in monocyte arrest on atherosclerotic endothelium and may also play a pathophysiological role in Alzheimer’s disease. Many chemokines are substrates for selective proteolysis at the amino-terminus by various proteases including dipeptidyl peptidase IV or matrix metalloproteases, resulting in truncated chemokine isoforms with different (both enhanced or reduced) bioactivities. The naturally occurring 68 aa N-terminal truncated isoform of mouse KC is reported to be a more potent synergistic growth stimulants for CFU-GM.

Entrez Gene IDs
2919 (Human); 14825 (Mouse); 81503 (Rat)
Alternate Names
CINC1; CINC-1; CXCL1; FSP; GRO alpha; GRO1; GROa; KC; MGSA; MGSA-a; MGSA-alpha; NAP-3; SCYB1

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Citations for Mouse CXCL1/GRO alpha /KC/CINC-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
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  1. KLK6/PAR1 Axis Promotes Tumor Growth and Metastasis by Regulating Cross-Talk between Tumor Cells and Macrophages
    Authors: YS Hwang, HJ Cho, ES Park, J Lim, HR Yoon, JT Kim, SR Yoon, H Jung, YK Choe, YH Kim, CH Lee, YT Kwon, BY Kim, HG Lee
    Cells, 2022-12-16;11(24):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  2. Neutrophil breaching of the blood vessel pericyte layer during diapedesis requires mast cell-derived IL-17A
    Authors: R Joulia, IM Guerrero-F, T Girbl, JA Coates, M Stein, L Vázquez-Ma, E Lynam, J Whiteford, M Schnoor, D Voehringer, A Roers, S Nourshargh, MB Voisin
    Nature Communications, 2022-11-17;13(1):7029.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  3. Essential roles of the cytokine oncostatin M in crosstalk between muscle fibers and immune cells in skeletal muscle after aerobic exercise
    Authors: T Komori, Y Morikawa
    The Journal of Biological Chemistry, 2022-11-09;0(0):102686.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Infiltration of peripheral immune cells into the olfactory bulb in a mouse model of acute nasal inflammation
    Authors: Hinami Asano, Sanae Hasegawa-Ishii, Ken Arae, Aki Obara, Geoffroy Laumet, Robert Dantzer et al.
    Journal of Neuroimmunology
  5. Metabolic lactate production coordinates vasculature development and progenitor behavior in the developing mouse neocortex
    Authors: X Dong, Q Zhang, X Yu, D Wang, J Ma, J Ma, SH Shi
    Nature Neuroscience, 2022-06-20;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Age-related changes in the local milieu of inflamed tissues cause aberrant neutrophil trafficking and subsequent remote organ damage
    Authors: Anna Barkaway, Loïc Rolas, Régis Joulia, Jennifer Bodkin, Tchern Lenn, Charlotte Owen-Woods et al.
    Immunity
  7. Increased cytokine expression in the choroid plexus stroma and epithelium in response to endotoxin-induced systemic inflammation in mice
    Authors: A Shimada, S Hasegawa-I
    Toxicology reports, 2021-03-10;8(0):520-528.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Role of Innate Inflammation in the Regulation of Tissue Remodeling during Tooth Eruption
    Authors: Y Makino, K Fujikawa, M Matsuki-Fu, S Inoue, M Nakamura
    Dentistry journal, 2021-01-12;9(1):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Visfatin Enhances Breast Cancer Progression through CXCL1 Induction in Tumor-Associated Macrophages
    Authors: YY Wang, HD Chen, S Lo, YK Chen, YC Huang, SC Hu, YC Hsieh, AC Hung, MF Hou, SF Yuan
    Cancers, 2020-11-26;12(12):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  10. Distinct Compartmentalization of the Chemokines CXCL1 and CXCL2 and the Atypical Receptor ACKR1 Determine Discrete Stages of Neutrophil Diapedesis
    Authors: Tamara Girbl, Tchern Lenn, Lorena Perez, Loïc Rolas, Anna Barkaway, Aude Thiriot et al.
    Immunity
  11. An IFN?/CXCL2 regulatory pathway determines lesion localization during EAE
    Authors: JS Stoolman, PC Duncker, AK Huber, DA Giles, JM Washnock-S, AM Soulika, BM Segal
    J Neuroinflammation, 2018-07-16;15(1):208.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. CXCL1 and CXCL2 Regulate NLRP3 Inflammasome Activation via G-Protein–Coupled Receptor CXCR2
    Authors: Monoranjan Boro, Kithiganahalli Narayanaswamy Balaji
    The Journal of Immunology
  13. Mycobacterium tuberculosis-triggered Hippo pathway orchestrates CXCL1/2 expression to modulate host immune responses
    Sci Rep, 2016-11-24;6(0):37695.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Neutralization
  14. Inducible Expression of CXCL1 within the Central Nervous System Amplifies Viral-Induced Demyelination.
    Authors: Marro B, Grist J, Lane T
    J Immunol, 2016-01-15;196(4):1855-64.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Astrocytes Are Primed by Chronic Neurodegeneration to Produce Exaggerated Chemokine and Cell Infiltration Responses to Acute Stimulation with the Cytokines IL-1 beta and TNF-alpha
    Authors: Edel Hennessy, Éadaoin W. Griffin, Colm Cunningham
    The Journal of Neuroscience
  16. P2RX7 sensitizes Mac-1/ICAM-1-dependent leukocyte-endothelial adhesion and promotes neurovascular injury during septic encephalopathy.
    Authors: Wang H, Hong L, Huang J, Jiang Q, Tao R, Tan C, Lu N, Wang C, Ahmed M, Lu Y, Liu Z, Shi W, Lai E, Wilcox C, Han F
    Cell Res, 2015-05-22;25(6):674-90.
    Species: Mouse
    Sample Types: In Vivo, Whole Tissue
    Applications: IHC, Neutralization
  17. Sphingolipid pathway regulates innate immune responses at the fetomaternal interface during pregnancy.
    Authors: Mizugishi K, Inoue T, Hatayama H, Bielawski J, Pierce J, Sato Y, Takaori-Kondo A, Konishi I, Yamashita K
    J Biol Chem, 2014-12-11;290(4):2053-68.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. CXCR2-mediated tumor-associated neutrophil recruitment is regulated by IFN-beta.
    Authors: Jablonska J, Wu C, Andzinski L, Leschner S, Weiss S
    Int J Cancer, 2013-10-31;134(6):1346-58.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  19. Altered regulation of Toll-like receptor responses impairs antibacterial immunity in the allergic lung.
    Authors: Habibzay, M, Saldana, J I, Goulding, J, Lloyd, C M, Hussell, T
    Mucosal Immunol, 2012-05-02;5(5):524-34.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  20. CCL20, gammadelta T cells, and IL-22 in corneal epithelial healing.
    Authors: Li Z, Burns AR, Miller SB, Smith CW
    FASEB J., 2011-04-25;25(8):2659-68.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  21. Intrinsic renal cell and leukocyte-derived TLR4 aggravate experimental anti-MPO glomerulonephritis.
    Authors: Summers SA, van der Veen BS, O'Sullivan KM
    Kidney Int., 2010-09-15;78(12):1263-74.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  22. Detrimental effect of the proteasome inhibitor, bortezomib in bacterial superantigen- and lipopolysaccharide-induced systemic inflammation.
    Authors: Tilahun AY, Theuer JE, Patel R
    Mol. Ther., 2010-04-06;18(6):1143-54.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  23. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010-02-08;120(3):907-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. Improved survival and reduced vascular permeability by eliminating or blocking 12/15-lipoxygenase in mouse models of acute lung injury (ALI).
    Authors: Zarbock A, Distasi MR, Smith E, Sanders JM, Kronke G, Harry BL, von Vietinghoff S, Buscher K, Nadler JL, Ley K
    J. Immunol., 2009-09-14;183(7):4715-22.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  25. Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
    Authors: Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ
    Proc. Natl. Acad. Sci. U.S.A., 2009-03-16;106(14):5954-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  26. Neuroprotection and remyelination after autoimmune demyelination in mice that inducibly overexpress CXCL1.
    Authors: Omari KM, Lutz SE, Santambrogio L, Lira SA, Raine CS
    Am. J. Pathol., 2008-12-18;174(1):164-76.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  27. Dachshund inhibits oncogene-induced breast cancer cellular migration and invasion through suppression of interleukin-8.
    Authors: Wu K, Katiyar S, Li A, Liu M, Ju X, Popov VM, Jiao X, Lisanti MP, Casola A, Pestell RG
    Proc. Natl. Acad. Sci. U.S.A., 2008-05-08;105(19):6924-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  28. High pressure promotes monocyte adhesion to the vascular wall.
    Authors: Riou S, Mees B, Esposito B, Merval R, Vilar J, Stengel D, Ninio E, van Haperen R, de Crom R, Tedgui A, Lehoux S
    Circ. Res., 2007-03-29;100(8):1226-33.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  29. High expression of ligands for chemokine receptor CXCR2 in alveolar epithelial neoplasia induced by oncogenic kras.
    Authors: Wislez M, Fujimoto N, Izzo JG, Hanna AE, Cody DD, Langley RR, Tang H, Burdick MD, Sato M, Minna JD, Mao L, Wistuba I, Strieter RM, Kurie JM
    Cancer Res., 2006-04-15;66(8):4198-207.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  30. Interleukin-1 in combination with oncostatin M up-regulates multiple genes in chondrocytes: implications for cartilage destruction and repair.
    Authors: Barksby HE, Hui W, Wappler I, Peters HH, Milner JM, Richards CD, Cawston TE, Rowan AD
    Arthritis Rheum., 2006-02-01;54(2):540-50.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  31. The chemokine KC, but not monocyte chemoattractant protein-1, triggers monocyte arrest on early atherosclerotic endothelium.
    Authors: Huo Y, Weber C, Forlow SB, Sperandio M, Thatte J, Mack M, Jung S, Littman DR, Ley K
    J. Clin. Invest., 2001-11-01;108(9):1307-14.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-P
  32. Tumor stem-like cell-derived exosomal RNAs prime neutrophils for facilitating tumorigenesis of colon cancer
    Authors: WL Hwang, HY Lan, WC Cheng, SC Huang, MH Yang
    J Hematol Oncol, 2019-01-25;12(1):10.

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