Mouse CXCL1/GRO alpha /KC/CINC-1 Antibody

Catalog #: MAB453R Datasheet

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB453R-100
MAB453R-SP

Chemotaxis Induced by CXCL1/GRO alpha /KC/CINC-1 and Neutralization by Mouse CXCL1/GRO alpha /KC/CINC-1 Antibody.

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All CXCL1/GRO alpha/KC/CINC-1 Antibodies

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Mouse CXCL1/GRO alpha /KC/CINC-1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CXCL-1/GRO alpha /KC/CINC‑1 in direct ELISAs.

Source

Recombinant Monoclonal Rat IgG_2A Clone # 48415R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived recombinant mouse CXCL-1/GRO alpha /KC/CINC‑1
Arg20-Lys96
Accession # P12850

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse CXCL1/GRO alpha /KC/CINC‑1 aa 20-96 (Catalog # 453-KC)

Mouse CXCL1/KC Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse CXCL1/GRO alpha /KC/CINC‑1 Biotinylated Antibody (Catalog # BAF453)

Standard: Recombinant Mouse CXCL1/KC Protein (Catalog # 453-KC)

Neutralization

Measured by its ability to neutralize CXCL1/GRO alpha /KC/CINC‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND₅₀) is typically 0.05-0.25 µg/mL in the presence of 30 ng/mL Recombinant Mouse CXCL1/GRO alpha /KC/CINC‑1 aa 20‑96.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by CXCL1/GRO alpha /KC/CINC-1 and Neutralization by Mouse CXCL1/GRO alpha /KC/CINC-1 Antibody. Recombinant Mouse CXCL1/GROa/KC/CINC-1 aa 20-96 (Catalog # 453-KC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/GROa/KC/CINC-1 aa 20-96 (30 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL1/GROa/KC/CINC-1 Monoclonal Antibody (Catalog # MAB453R). The ND₅₀ is typically0.05-0.25 µg/mL.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL1/GRO alpha/KC/CINC-1

CXCL1, also known as KC and GRO alpha, is a CXC family chemokine that is expressed in fibroblasts, macrophages, and endothelial cells and interacts with CXCR2. CXCL1 is a potent neutrophil attractant and activator. It also promotes angiogenesis by inducing VEGF production by neutrophils. N-terminally truncated CXCL1 demonstrates increased potency in CXCR2 signaling. Mature mouse CXCL1 shares 96% and 64% aa sequence identity with rat and human CXCL1, respectively.

Entrez Gene IDs

2919 (Human); 14825 (Mouse); 81503 (Rat)

Alternate Names

CINC1; CINC-1; CXCL1; FSP; GRO alpha; GRO1; GROa; KC; MGSA; MGSA-a; MGSA-alpha; NAP-3; SCYB1