Mouse CXCL1/GRO alpha /KC/CINC-1 Antibody
Mouse CXCL1/GRO alpha /KC/CINC-1 Antibody Summary
Asn29-Lys96
Accession # P12850
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CXCL1/GRO alpha /KC/CINC‑1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either (A) treated with 1 µg/mL LPS and Brefeldin A for 4 hours or (B) untreated were stained with Rabbit Anti-Mouse CXCL1/GROa/KC/CINC-1 Monoclonal Antibody (Catalog # MAB4532) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110) and Rat Anti-Mouse TNF-a Fluorescein-conjugated Monoclonal Antibody (Catalog # IC410F). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL1/GRO alpha/KC/CINC-1
KC, a member of the alpha (CXC) chemokine subfamily, was initially identified as an immediate early gene induced in mouse fibroblasts by platelet‑derived growth factor. KC cDNA encodes a 96 amino acid (aa) residue precursor protein with a predicted secretory signal peptide that is removed to yield the mature protein. The protein sequence of mouse KC shows approximately 63% identity to that of mouse MIP-2. KC is also approximately 60% identical to the human GROs. It has been suggested that mouse KC and MIP-2 are the orthologs of the human GROs and rat CINCs. In addition to mouse fibroblasts, KC is expressed in macrophages and endothelial cells. Mouse KC is a potent neutrophil attractant and activator. The functional receptor for KC has been identified as CXCR2. Based on the pattern of KC expression in a number of inflammatory disease models, KC appears to have an important role in inflammation. KC was found to be involved in monocyte arrest on atherosclerotic endothelium and may also play a pathophysiological role in Alzheimer’s disease. Many chemokines are substrates for selective proteolysis at the amino-terminus by various proteases including dipeptidyl peptidase IV or matrix metalloproteases, resulting in truncated chemokine isoforms with different (both enhanced or reduced) bioactivities. The naturally occurring 68 aa N-terminal truncated isoform of mouse KC is reported to be a more potent synergistic growth stimulant for CFU-GM.
Product Datasheets
Citations for Mouse CXCL1/GRO alpha /KC/CINC-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Alveolar epithelial cells mitigate neutrophilic inflammation in lung injury through regulating mitochondrial fatty acid oxidation
Authors: Chung, KP;Cheng, CN;Chen, YJ;Hsu, CL;Huang, YL;Hsieh, MS;Kuo, HC;Lin, YT;Juan, YH;Nakahira, K;Chen, YF;Liu, WL;Ruan, SY;Chien, JY;Plataki, M;Cloonan, SM;Carmeliet, P;Choi, AMK;Kuo, CH;Yu, CJ;
Nature communications
Species: Transgenic Mouse
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
Endothelial Cdk5 deficit leads to the development of spontaneous epilepsy through CXCL1/CXCR2-mediated reactive astrogliosis
Authors: Xiu-xiu Liu, Lin Yang, Ling-xiao Shao, Yang He, Gang Wu, Yu-huan Bao et al.
Journal of Experimental Medicine
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