Mouse Complement Factor H Alexa Fluor® 350-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Complement Factor H

Factor H is a 155 kDa glycoprotein that provides critical negative regulation of the alternative pathway complement cascade. It is secreted by Kupffer cells, hepatocytes, vascular endothelial cells, and platelets and circulates in the serum at high concentration (1). Factor H is composed of 20 SCR (short consensus repeats), each of which consists of approximately 60 amino acids with four invariant Cys residues (2). Factor H interacts with cell surface polyanions including heparin and sialoglycoproteins (3-6), and immobilized Factor H supports the CD11b/CD18 integrin-dependent adhesion of neutrophils (7). It prevents local complement activation by sequestering complement component C3b, accelerating the decay of C3 and C5 convertases, and functioning as a cofactor for the C3b inactivator, Factor I (1, 3, 6, 8). This recombinant protein corresponds to SCR15-20 which encompass the primary binding sites for heparin and C3b as well as for the peptide hormone adrenomedullin (4, 9-11). Within SCR15-20, mouse Factor H shares 60% and 80% amino acid sequence identity with human and rat Factor H, respectively. Dozens of mutations clustered in SCR15-20 are associated with atypical hemolytic uremic syndrome, a disorder characterized by anemia, thrombocytopenia, and renal failure (12). Binding of Factor H to tumor cell-associated dentin matrix protein 1, bone sialoprotein, or osteopontin results in the protection of that cell from complement mediated lysis (13, 14). A variety of pathogenic microbes also express Factor H binding molecules that interfere with immune clearance of the infection (15).

Product Datasheets

Related Research Areas

• Alpha Granule Soluble Molecules
• Complement Pathway
• Serine Proteases and Regulators