Mouse Complement Factor D/Adipsin Antibody

Detection of Mouse Complement Factor D/Adipsin by Western Blot. Western blot shows lysates of mouse plasma. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Complement Factor D/Adipsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5430) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Complement Factor D/Adipsin at approximately 45kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of Mouse Complement Factor D/Adipsin by Western Blot Bone marrow (BM) Adipsin induces bone marrow adiposity expansion during aging.(A) Immunoblot of Adipsin and Adiponectin from plasma of chow-fed male mice at 6, 24, and 76 weeks of age (L.C. = Coomassie staining of the membrane). (B, C) qPCR analyses of gene expression in the BM from tibia (B) and Cfd expression in the epididymal white adipose tissue and subcutaneous white adipose tissue (C) from chow-fed male mice at 26 and 78 weeks of age (n = 5, 5). *p<0.05 for young vs. aging mice. (D–I) Chow-fed 1-year-old male mice. WT (n = 10) and Adipsin KO (n = 9). (D) Representative osmium tetroxide staining and (E) quantification of femoral MAT; (F, G) femoral bone mineral density (BMD) in the cortical (F) and trabecular (G) regions, and (H, I) bone volume normalized by total voume in the cortical (H) and trabecular (I) regions of the femur determined by μCT scans. **p<0.01 for WT vs. Adipsin KO mice. Data represent mean ± SEM. Two-tailed Student’s t-tests were used for statistical analyses.Metabolic phenotyping of Adipsin KO mice during aging.Chow-fed 1-year-old male mice. WT (n = 10) and Adipsin KO (n = 9). (A) Body weight and composition assessed by EchoMRI; (B) insulin tolerance test; (C) GTT; (D) hematoxylin and eosin staining of femoral marrow adipose tissue. (E, F) Average trabecular number (E) and cortical thickness (F) of femurs determined by μCT scans. Data represent mean ± SEM. Two-tailed Student’s t-test was used for statistical analyses. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34155972), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Complement Factor D/Adipsin by Western Blot Adipsin is robustly induced in the bone marrow (BM) during bone marrow adipose tissue (BMAT) expansion. (D) immunoblot of plasma Adipsin&Adiponectin (APN) – Coomassie staining of the membrane was used as loading control (L.C.) (n = 4, 4);&immunoblot of Adipsin from bone – HSP90 was used as the loading control (n = 4, 4). Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34155972), licensed under a CC-BY license. Not internally tested by R&D Systems.