Mouse Coagulation Factor III/Tissue Factor PE-conjugated Antibody
Mouse Coagulation Factor III/Tissue Factor PE-conjugated Antibody Summary
Ala29-Glu251
Accession # P20352
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Coagulation Factor III/ Tissue Factor in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line treated with LPS was stained with Goat Anti-Mouse Coagulation Factor III/Tissue Factor PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3178P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Coagulation Factor III/Tissue Factor
Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is an integral membrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). Synthesized as a 294 amino acid precursor, mouse TF consists of a signal peptide (residues 1-28) and the mature chain (residues 29-294). As a type I membrane protein, it contains a transmembrane region (residues 252-274) and a cytoplasmic tail (residues 275-294) (3, 4). The purified rmTF corresponds to the ectodomain (residues 29-251) and is potent in activating thermolysin-processed rmCoagulation Factor VII (R&D Systems, Catalog # 3305-SE) under the conditions described in the Activity Assay Protocol.
- Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) Academic Press, San Diego, p. 1659.
- Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009.
- Ranganathan, G. et al. (1991) J. Biol. Chem. 266:496.
- Hartzell, S. (1989) Mol. Cell. Biol. 9:2567.
Product Datasheets
Citations for Mouse Coagulation Factor III/Tissue Factor PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Mammalian adipogenesis regulator (Areg) cells use retinoic acid signalling to be non‐ and anti‐adipogenic in age‐dependent manner
Authors: Magda Zachara, Pernille Y Rainer, Horia Hashimi, Julie M Russeil, Daniel Alpern, Radiana Ferrero et al.
The EMBO Journal
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Alternative activation of human macrophages enhances tissue factor expression and production of extracellular vesicles
Authors: PJ Hohensinne, J Mayer, J Kichbacher, J Kral-Point, B Thaler, C Kaun, L Hell, P Haider, M Mussbacher, JA Schmid, S Stojkovic, S Demyanets, MB Fischer, K Huber, K Wöran, C Hengstenbe, WS Speidl, R Oehler, I Pabinger, J Wojta
Haematologica, 2021-02-01;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
SENP3 in monocytes/macrophages up-regulates tissue factor and mediates lipopolysaccharide-induced acute lung injury by enhancing JNK phosphorylation
Authors: X Chen, Y Lao, J Yi, J Yang, S He, Y Chen
J. Cell. Mol. Med., 2020-03-31;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Damage- and pathogen-associated molecular patterns play differential roles in late mortality after critical illness
Authors: John Eppensteiner, Jean Kwun, Uwe Scheuermann, Andrew Barbas, Alexander T. Limkakeng, Maggie Kuchibhatla et al.
JCI Insight
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Androgen receptor dampens tissue factor expression via NF-?B and EGR1
Authors: B Hoesel, M Mussbacher, B Dikorman, M Salzmann, A Assinger, L Hell, J Thaler, J Basílio, B Moser, U Resch, H Paar, N Mackman, JA Schmid
J. Thromb. Haemost., 2018-03-13;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Chimeric antigen receptor-modified T Cells inhibit the growth and metastases of established tissue factor-positive tumors in NOG mice
Authors: Q Zhang, H Wang, H Li, J Xu, K Tian, J Yang, Z Lu, J Zheng
Oncotarget, 2017-02-07;8(6):9488-9499.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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