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Mouse CD8 alpha Biotinylated Antibody

Catalog #: BAM116
2 Citations Datasheet
Catalog # Availability Size / Price Qty
BAM116
Detection of CD8 alpha in Mouse Splenocytes by Flow Cytometry
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Product Details
Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD8 alpha Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD8 alpha. Reacts with alpha chains of mouse CD8 (Bierer, B.E. et al. (1989) Annu. Rev. Immunol. 7:579).
Source
Monoclonal Rat IgG2A Clone # 53-6.7
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse thymus and spleen cells
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD8 alpha antibody in Mouse Splenocytes antibody by Flow Cytometry View Larger

Detection of CD8 alpha in Mouse Splenocytes by Flow Cytometry Mouse splenocytes were stained with (A) Rat Anti-Mouse Biotinylated CD8 alpha Monoclonal Antibody (Catalog # BAM116) or (B) Rat IgG2A Biotinylated isotype control antibody (Catalog # IC006B) followed by Streptavidin-Phycoerythrin (Catalog # F0040) and Rat Anti-Mouse CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB4841A). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry CD8a antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

CD8 alpha in Mouse Splenocytes. CD8a was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD8a Biotinylated Monoclonal Antibody (Catalog # BAM116) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry CD8a antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

CD8 alpha in Mouse Splenocytes. CD8a was detected in immersion fixed mouse splenocytes using 10 µg/mL Rat Anti-Mouse CD8a Biotinylated Monoclonal Antibody (Catalog # BAM116) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD8

CD8, also known as Ly-2, is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes. Mouse CD8 (containing an alpha /Ly-2 or alpha ′/Lyt-2 chain) is an antigen co‑receptor on the T cell surface which interacts with MHC I molecules on antigen presenting cells (1). CD8 alpha beta heterodimer is expressed on a subpopulation of mature T cells (2, 3). CD8 alpha, without CD8 beta, has been detected on subsets of gamma δ TCR-bearing T cells (4), intestinal intrathymic lymphocytes (5, 6) and dendritic cells (7, 8).

References
  1. Bierer, B.E. et al. (1989) Annu. Rev. Immunol. 7:579.
  2. Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
  3. Hayakawa, K. et al. (1994) Science 263:1131.
  4. MacDonald, H.R. et al. (1990) Eur. J. Immunol. 20:927.
  5. Rocha, B. et al. (1992) Immunol. Today 13:449.
  6. Wang, J. and J.R. Klein (1994) Science 265:1860.
  7. Vermec, D. et al. (1992) J. Exp. Med. 176:47.
  8. Suss, G. and K. Shortman (1996) J. Exp. Med. 183:1789.
Entrez Gene IDs
925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)
Alternate Names
CD_antigen: CD8a; CD8 antigen, alpha polypeptide (p32); CD8; CD8a molecule; CD8A; Leu2 T-lymphocyte antigen; LEU2; MAL; OKT8 T-cell antigen; p32; T cell co-receptor; T8 T-cell antigen; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2

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Citations for Mouse CD8 alpha Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Cross-protective peptide vaccine against influenza A viruses developed in HLA-A*2402 human immunity model.
    Authors: Ichihashi T, Yoshida R, Sugimoto C
    PLoS ONE, 2011-09-19;6(9):e24626.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  2. Blockade of transforming growth factor-{beta} signaling in tumor-reactive CD8(+) T cells activates the antitumor immune response cycle.
    Authors: Zhang Q, Yang XJ, Kundu SD, Pins M, Javonovic B, Meyer R, Kim SJ, Greenberg NM, Kuzel T, Meagher R, Guo Y, Lee C
    Mol. Cancer Ther., 2006-07-01;5(7):1733-43.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Affinity Purification

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