Home / CD4 / Mouse CD4 Biotinylated Antibody

Mouse CD4 Biotinylated Antibody

Catalog #: BAM554
3 Citations Datasheet
Catalog # Availability Size / Price Qty
BAM554
CD4 in Mouse Splenocytes.
1 Image
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD4 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Recognizes mouse CD4 (1‑3).
Source
Monoclonal Rat IgG2B Clone # GK1.5
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen

Mouse CTL clone V4

Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Mouse splenocytes
Immunohistochemistry
Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280., Zheng, B.S. et al. (1996) J. Exp. Med. 184:1083.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry CD4 antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

CD4 in Mouse Splenocytes. CD4 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD4 Biotinylated Monoclonal Antibody (Catalog # BAM554) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI(blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD4

CD4 is a type I membrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed predominantly on thymocytes and a subset of mature T lymphocytes. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. CD4 also has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

References
  1. Dialynas, D.P. et al. (1983) J. Immunol. 131:2445.
  2. Dialynas, D.P. et al. (1983) Immunol. Rev. 74:29.
  3. Bendelac, A. (1995) Curr. Opin. Immunol. 7:367.
Entrez Gene IDs
920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)
Alternate Names
CD_antigen: CD4; CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

Product Datasheets

You must select a language.

x
Product Datasheet

Citations for Mouse CD4 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. CD4+ T cells are activated in regional lymph nodes and migrate to skin to initiate lymphedema
    Authors: GD García Nor, CL Ly, DA Cuzzone, RP Kataru, GE Hespe, JS Torrisi, JJ Huang, JC Gardenier, IL Savetsky, MD Nitti, JZ Yu, S Rehal, BJ Mehrara
    Nat Commun, 2018-05-17;9(1):1970.
  2. T helper 2 differentiation is necessary for development of lymphedema
    Authors: Catherine L. Ly, Gabriela D. García García Nores, Raghu P. Kataru, Babak J. Mehrara
    Translational Research
  3. Impaired salivary gland function in NOD mice: association with changes in cytokine profile but not with histopathologic changes in the salivary gland.
    Authors: Jonsson MV, Delaleu N, Brokstad KA, Berggreen E, Skarstein K
    Arthritis Rheum., 2006-07-01;54(7):2300-5.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse CD4 Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Mouse CD4 Biotinylated Antibody and earn rewards!

Have you used Mouse CD4 Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review