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Mouse CD4 Antibody

Catalog #: AF554
8 Citations Datasheet
Catalog # Availability Size / Price Qty
AF554
AF554-SP
Product Details
Citations (8)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD4 in direct ELISAs and Western blots. In these formats, less than 1% cross‑reactivity with recombinant human CD4, recombinant feline CD4, and recombinant canine CD4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD4
Lys27-Thr394
Accession # P06332
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CD4

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD4

CD4 (Cluster of Differentiation #4; also L3T4) is a 55 kDa member of the Ig Superfamily of molecules.  It is expressed on a variety of hematopoietic cell types, including immature thymocytes, a number of variable phenotype single positive T cells (i.e.- CD25+; CD39+; Th1, 2 and 17 type cells), stimulated CD8+ T cells, pluripotent bone marrow stem cells, and NKT cells.  Although CD4 is a standard phenotype marker, functionally, the molecule is known to bind to APC MHC class II, and to associate with adjacent CD3 molecules to link p56lck with the lymphocyte TCR:CD3 complex.  Mature mouse CD4 is a 431 amino acid (aa) type I transmembrane glycoprotein.  It contains a 368 aa extracellular region (aa 27-394) plus a 40 aa cytoplasmic domain (aa 418-457).  The extracellular region contains one V-type (aa 27-128) and three C2-type Ig-like domains (aa 129-374).  There is one alternative start site at Met241 that is utilized in brain.  Over aa 27-394, mouse CD4 shares 53% and 72% aa identity with human and rat CD4, respectively.        

Entrez Gene IDs
920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)
Alternate Names
CD_antigen: CD4; CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

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Citations for Mouse CD4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Soluble RAGE Prevents Type 1 Diabetes Expanding Functional Regulatory T Cells
    Authors: Sherman S. Leung, Danielle J. Borg, Domenica A. McCarthy, Tamar E. Boursalian, Justen Cracraft, Aowen Zhuang et al.
    Diabetes
  2. Kidney disease risk factors do not explain impacts of low dietary protein on kidney function and structure
    Authors: Amelia K. Fotheringham, Samantha M. Solon-Biet, Helle Bielefeldt-Ohmann, Domenica A. McCarthy, Aisling C. McMahon, Kari Ruohonen et al.
    iScience
  3. Selective inhibition of HDAC3 targets synthetic vulnerabilities and activates immune surveillance in lymphoma.
    Authors: Patrizia Mondello, Saber Tadros, Matt Teater, Lorena Fontan, Aaron Y. Chang, Neeraj Jain et al.
    Cancer Discovery
  4. The Polycomb Repressor Complex 1 Drives Double-Negative Prostate Cancer Metastasis by Coordinating Stemness and Immune Suppression
    Authors: Wenjing Su, Hyun Ho Han, Yan Wang, Boyu Zhang, Bing Zhou, Yuanming Cheng et al.
    Cancer Cell
  5. Non-conventional Inhibitory CD4+Foxp3−PD-1hi T Cells as a Biomarker of Immune Checkpoint Blockade Activity
    Authors: Roberta Zappasodi, Sadna Budhu, Matthew D. Hellmann, Michael A. Postow, Yasin Senbabaoglu, Sasikanth Manne et al.
    Cancer Cell
  6. Diphtheria toxin–mediated ablation of lymphatic endothelial cells results in progressive lymphedema
    Authors: Jason C. Gardenier, Geoffrey E. Hespe, Raghu P. Kataru, Ira L. Savetsky, Jeremy S. Torrisi, Gabriela D. García Nores et al.
    JCI Insight
  7. Determinants in the beta and delta subunit cytoplasmic loop regulate Golgi trafficking and surface expression of the muscle acetylcholine receptor.
    Authors: Rudell J, Borges L, Rudell J, Beck K, Ferns M
    J Biol Chem, 2013-11-15;289(1):203-14.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  8. Timing of estrogen replacement influences atherosclerosis progression and plaque leukocyte populations in ApoE-/- mice.
    Authors: Cann JA, Register TC, Adams MR, St Clair RW, Espeland MA, Williams JK
    Atherosclerosis, 2008-02-21;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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