Mouse CD14 Biotinylated Antibody Summary
Ala18-Pro345
Accession # Q4FJP7
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD14
CD14 is a 55 kDa cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The mouse CD14 cDNA encodes a 366 amino acid (aa) residue precursor protein with a 15 aa signal peptide and a C-terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Mouse CD14 has five potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of mouse CD14 is approximately 65% and 82% identical to the human and rat proteins, respectively. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP). The toll-like-receptors have also been implicated in the transduction of CD14-LPS signals. Similar to other GPI-anchored proteins, soluble CD14 can be released from the cell surface by phosphatidyinositol-specific phospholipase C. Soluble CD14 has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS-mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14.
- Wright, S.D. et al. (1990) Science 249:1431.
- Pugin, J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:2744.
- Beutler, B. (2000) Current Opinion in Immunology 12:20.
- Stelter, F. (2000) Chem. Immunol. 74:25.
Product Datasheets
Citations for Mouse CD14 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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A novel role of the lumican core protein in bacterial lipopolysaccharide-induced innate immune response.
Authors: Wu F, Vij N, Roberts L, Lopez-Briones S, Joyce S, Chakravarti S
J. Biol. Chem., 2007-07-05;282(36):26409-17.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Minimally modified LDL binds to CD14, induces macrophage spreading via TLR4/MD-2, and inhibits phagocytosis of apoptotic cells.
Authors: Miller YI, Viriyakosol S, Binder CJ, Feramisco JR, Kirkland TN, Witztum JL
J. Biol. Chem., 2002-11-06;278(3):1561-8.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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