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Mouse CCR1 Antibody

Catalog #: MAB5986
5 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB5986
MAB5986-SP
Detection of Mouse CCR1 by Western Blot.
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Product Details
Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCR1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCR1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CCR4, 5, or 8 is observed.
Source
Monoclonal Rat IgG2B Clone # 643854
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse CCR1
Extracellular regions (aa 1-34, aa 92-107, aa 172-192, aa 265-281)
Accession # P51675
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse CCR1 antibody by Western Blot. View Larger

Detection of Mouse CCR1 by Western Blot. Western blot shows lysates of mouse heart tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse CCR1 Monoclonal Antibody (Catalog # MAB5986) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CCR1 at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of CCR1 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of CCR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse CCR1 Monoclonal Antibody (Catalog # MAB5986, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR1

CCR1 (C-C chemokine receptor 1; also MIP-1a receptor and CD191) is a 43 kDa member of the GPCR #1 family of transmembrane proteins. Mouse CCR1 is expressed on osteoclasts, IL-13+ T cells, neutrophils, bone marrow-derived mast cells, eosinophils, monocytes and vascular intimal smooth muscle cells. Multiple chemokines are reported to bind to CCR1. MIP-1a/CCL3 and RANTES/CCL5 are natural ligands, while CCL15, CCL9 and CCL23 are potent angonists after N-terminal processing. Mouse CCR1 is a 7-transmembrane protein that is 355 amino acids (aa) in length. It contains a 34 aa N-terminal extracellular domain plus a
50 aa C-terminal cytoplasmic tail. Over aa sequences 1-34, 92-107, 172-192 and 265-281 collectively, mouse CCR1 shares 76% and 89% aa identity with human and rat CCR1, respectively. 

Entrez Gene IDs
1230 (Human); 12768 (Mouse); 57301 (Rat)
Alternate Names
C-C chemokine receptor type 1; CC-CKR-1; CCR1; CCR-1; CD191 antigen; CD191; chemokine (C-C motif) receptor 1; CKR1; CKR-1; CMKBR1SCYAR1; CMKR1; HM145C-C CKR-1; LD78 receptor; Macrophage inflammatory protein 1-alpha receptor; MIP-1alpha-R; MIP1aR; RANTES receptor; RANTES-R

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Citations for Mouse CCR1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Luteolin Alleviates Liver Fibrosis in Rat Hepatic Stellate Cell HSC-T6: A Proteomic Analysis
    Authors: Batudeligen, ;Han, Z;Chen, H;Narisu, ;Xu, Y;Anda, ;Han, G;
    Drug design, development and therapy
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. CCL7 contributes to angiotensin II-induced abdominal aortic aneurysm by promoting macrophage infiltration and pro-inflammatory phenotype
    Authors: C Xie, F Ye, N Zhang, Y Huang, Y Pan, X Xie
    Journal of Cellular and Molecular Medicine, 2021-06-29;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. The role of CCR1 and therapeutic effects of anti‐CCL3 antibody in herpes simplex virus‐induced Behçet's disease mouse model
    Authors: Hasan M. Sayeed, Eun‐So Lee, Hae‐Ok Byun, Seonghyang Sohn
    Immunology
  4. CCR1 plays a critical role in modulating pain through hematopoietic and non-hematopoietic cells.
    Authors: Lewis, Nuruddee, Muthukumarana, Akalushi, Fogal, Steven E, Corradini, Laura, Stefanopoulos, Dimitria, Adusumalli, Prathima, Pelletier, Josephin, Panzenbeck, Mark, Berg, Karen, Canfield, Melissa, Cook, Brian N, Razavi, Hossein, Kuzmich, Daniel, Anderson, Shawn, Allard, Devan, Harrison, Paul, Grimaldi, Christin, Souza, Donald, Harcken, Christia, Fryer, Ryan M, Modis, Louise K, Brown, Maryanne
    PLoS ONE, 2014-08-29;9(8):e105883.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. MIP-1alpha/CCL3-mediated maintenance of leukemia-initiating cells in the initiation process of chronic myeloid leukemia.
    Authors: Baba T, Naka K, Morishita S, Komatsu N, Hirao A, Mukaida N
    J Exp Med, 2013-10-28;210(12):2661-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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