Mouse CCL3/MIP-1 alpha Antibody Summary
Ala24-Ala92
Accession # Q5QNW0
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Chemotaxis Induced by CCL3/MIP‑1 alpha and Neutrali-zation by Mouse CCL3/MIP‑1 alpha Antibody. Recombinant Mouse CCL3/MIP-1a (Catalog # 450-MA) chemoattracts BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL3/MIP-1a (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL3/MIP-1a Polyclonal Antibody (Catalog # AB-450-NA). The ND50 is typically 0.3-1 µg/mL.
View Larger
Detection of Mouse CCL3/MIP-1 alpha by Immunocytochemistry/ Immunofluorescence Effects of neutralizing anti-CCL3 antibody on the number of macrophages at the damaged site and the decrease in area of bone defect on day 4 in uPA+/+ and uPA-/- mice.(A) Microphotographs of immunostaining for F4/80 at the damaged site on day 4 after a femoral bone defect in uPA+/+ and uPA-/- mice treated with normal IgG (Cont) or neutralizing anti-CCL3 antibody (Anti-CCL3). The results represent experiments performed on 5 mice in each group. Scale bars indicate 50 μm. (B) Quantification of the number of F4/80-positive cells per 0.1 mm2 in the microscopic fields in the damaged site on day 4 after a femoral bone defect in uPA+/+ and uPA-/- mice treated with normal IgG or neutralizing anti-CCL3 antibody. The data represent the mean ± SEM of 5 mice. (C) Quantification of the bone defect area as assessed by qCT on days 1 and 4 in uPA+/+ and uPA-/- mice treated with normal IgG or neutralizing anti-CCL3 antibody. The data represent the mean ± SEM from 5 mice. **P < 0.01 and *P < 0.05 (Tukey’s test). Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0123982), licensed under a CC-BY license. Not internally tested by R&D Systems.
View Larger
Detection of Mouse CCL3/MIP-1 alpha by Immunocytochemistry/ Immunofluorescence Effects of neutralizing anti-CCL3 antibody on the number of macrophages at the damaged site and the decrease in area of bone defect on day 4 in uPA+/+ and uPA-/- mice.(A) Microphotographs of immunostaining for F4/80 at the damaged site on day 4 after a femoral bone defect in uPA+/+ and uPA-/- mice treated with normal IgG (Cont) or neutralizing anti-CCL3 antibody (Anti-CCL3). The results represent experiments performed on 5 mice in each group. Scale bars indicate 50 μm. (B) Quantification of the number of F4/80-positive cells per 0.1 mm2 in the microscopic fields in the damaged site on day 4 after a femoral bone defect in uPA+/+ and uPA-/- mice treated with normal IgG or neutralizing anti-CCL3 antibody. The data represent the mean ± SEM of 5 mice. (C) Quantification of the bone defect area as assessed by qCT on days 1 and 4 in uPA+/+ and uPA-/- mice treated with normal IgG or neutralizing anti-CCL3 antibody. The data represent the mean ± SEM from 5 mice. **P < 0.01 and *P < 0.05 (Tukey’s test). Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0123982), licensed under a CC-BY license. Not internally tested by R&D Systems.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL3/MIP-1 alpha
The macrophage inflammatory proteins 1 alpha and 1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP-1 alpha shares approximately 77% and 70% amino acid identity with human MIP-1 alpha and mouse MIP-1 beta, respectively.
MIP‑1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP-1 proteins are members of the beta (C-C) subfamily of chemokines.
Both MIP-1 alpha and MIP-1 beta are monocyte chemoattractants in vitro. Additionally, the MIP-1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP-1 alpha and MIP-1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. MIP-1 alpha has also been shown to attract B cells as well as eosinophils. MIP-1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP-1 alpha has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. In the same assays, MIP-1 beta was reported to be much less active. The functional receptor for MIP-1 alpha has been identified as CCR1 and CCR5.
Product Datasheets
Citations for Mouse CCL3/MIP-1 alpha Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
7
Citations: Showing 1 - 7
Filter your results:
Filter by:
-
Prevention of CaCl2-induced aortic inflammation and subsequent aneurysm formation by the CCL3-CCR5 axis
Authors: Y Ishida, Y Kuninaka, M Nosaka, A Kimura, A Taruya, M Furuta, N Mukaida, T Kondo
Nat Commun, 2020-11-25;11(1):5994.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Prospective clinical testing and experimental validation of the Pediatric Sepsis Biomarker Risk Model
Authors: Hector R. Wong, J. Timothy Caldwell, Natalie Z. Cvijanovich, Scott L. Weiss, Julie C. Fitzgerald, Michael T. Bigham et al.
Science Translational Medicine
-
Streptozotocin?induced diabetic mice exhibit reduced experimental choroidal neovascularization but not corneal neovascularization
Authors: G Liu, L Chen, Q Cai, H Wu, Z Chen, X Zhang, P Lu
Mol Med Rep, 2018-09-03;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: Western Blot -
Chemokines CCL2, 3, 14 stimulate macrophage bone marrow homing, proliferation, and polarization in multiple myeloma
Authors: Yi Li, Yuhuan Zheng, Tianshu Li, Qiang Wang, Jianfei Qian, Yong Lu et al.
Oncotarget
-
The Tissue Fibrinolytic System Contributes to the Induction of Macrophage Function and CCL3 during Bone Repair in Mice.
Authors: Kawao N, Tamura Y, Horiuchi Y, Okumoto K, Yano M, Okada K, Matsuo O, Kaji H
PLoS ONE, 2015-04-20;10(4):e0123982.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Definition of Key Variables for the Induction of Optimal NY-ESO-1–Specific T Cells in HLA Transgene Mice
Authors: Alexandre Johannsen, Raphaël Genolet, Daniel F. Legler, Sanjiv A. Luther, Immanuel F. Luescher
The Journal of Immunology
-
Protection against inflammation- and autoantibody-caused fetal loss by the chemokine decoy receptor D6.
Authors: Martinez de la Torre Y, Buracchi C, Borroni EM, Dupor J, Bonecchi R, Nebuloni M, Pasqualini F, Doni A, Lauri E, Agostinis C, Bulla R, Cook DN, Haribabu B, Meroni P, Rukavina D, Vago L, Tedesco F, Vecchi A, Lira SA, Locati M, Mantovani A
Proc. Natl. Acad. Sci. U.S.A., 2007-02-05;104(7):2319-24.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Mouse CCL3/MIP-1 alpha Antibody
There are currently no reviews for this product. Be the first to review Mouse CCL3/MIP-1 alpha Antibody and earn rewards!
Have you used Mouse CCL3/MIP-1 alpha Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
