Mouse CCL27/CTACK Antibody

Catalog #: MAB7251
2 Citations 1 Review Datasheet

Catalog #	Availability	Size / Price	Qty
MAB7251
MAB7251-SP

All CCL27/CTACK Antibodies

Product Details

Citations (2)

FAQs

Supplemental Products

Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCL27/CTACK Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCL27/CTACK in direct ELISAs and Western blots.

Source

Monoclonal Rat IgG_2A Clone # 68706

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human CCL27/CTACK
Leu26-Asn120
Accession # NP_035466

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse CCL27/CTACK (Catalog # 725-CK)

Immunohistochemistry

8-25 µg/mL

Perfusion fixed frozen sections of mouse skin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL27/CTACK

CCL27, also known as CTACK (cutaneous T cell-attracting chemokine), ALP, ILC, and ESkine, is a member of the CC family of chemokines (1). Mature mouse CCL27 is a 95 amino acid (aa) protein that shares 57% aa and 87% aa sequence identity with human and rat CCL27, respectively (2). It shares 18-31% aa sequence identity with other mouse CC chemokines. An alternately spliced form of mouse CCL27, known as PESKY, is localized to the nucleus and promotes cellular migration (3). CCL27 is constitutively expressed by keratinocytes and is upregulated by inflammatory stimuli and in wounded skin (4-7). CCL27 binds the chemokine receptor CCR10, glycosaminoglycans in the extracellular matrix, sulfated tyrosine residues on PSGL-1, and determinants on the surface of fibroblasts and endothelial cells (5, 7‑9). CCL27 cooperates with CCL17/TARC in inducing the migration of cutaneous lymphocyte antigen (CLA) positive memory T cells to the skin during inflammation (4, 6, 10-12). Endothelial cell-bound CCL27 can mediate the adhesion of those cells to CLA⁺ T cells (6). CCL27 also induces the migration of keratinocyte precursors from bone marrow to the skin, thereby promoting wound healing (7). In humans, serum CCL27 levels are elevated and correlate with disease severity in atopic dermatitis, psoriasis vulgaris, and mycosis fungoides (13-15).

References

Kunkel, L. and E.C. Butcher (2002) Immunity 16:1.
Hromas, R. et al. (1999) Biochem. Biophys. Res. Commun. 258:737.
Gortz, A. et al. (2002) J. Immunol. 169:1387.
Morales, J. et al. (1999) Proc. Natl. Acad. Sci. 96:14470.
Homey, B. et al. (2000) J. Immunol. 164:3465.
Homey, B. et al. (2002) Nat. Med. 8:157.
Inokuma, D. et al. (2006) Stem Cells 24:2810.
Jarmin, D. et al. (2000) J. Immunol. 164:3460.
Hirata, T. et al. (2004) J. Biol. Chem. 279:51775.
Vestergaard, C. et al. (2004) Exp. Dermatol. 13:551.
Reiss, Y. et al. (2001) J. Exp. Med. 194:1541.
Soler, D. et al. (2003) Blood 101:1677.
Kakinuma, T. et al. (2003) J. Allergy Clin. Immunol. 111:592.
Hijnen, D. et al. (2004) J. Allergy Clin. Immunol. 113:334.
Fujita, Y. et al. (2006) Clin. Cancer Res. 12:2670.

Entrez Gene IDs

10850 (Human); 20301 (Mouse); 362505 (Rat)

Alternate Names

ALP; C-C motif chemokine 27; CCL27; chemokine (C-C motif) ligand 27; CTACK; CTACKSmall-inducible cytokine A27; CTAK; cutaneous T-cell attracting chemokine; Cutaneous T-cell-attracting chemokine; ESKINE; IL-11 Ralpha-locus chemokine; ILC; ILCCC chemokine ILC; PESKY; SCYA27IL-11 R-alpha-locus chemokine; skinkine; small inducible cytokine subfamily A (Cys-Cys), member 27

Product Datasheets

Product Datasheet

Related Research Areas

Citations for Mouse CCL27/CTACK Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Plasmids encoding the mucosal chemokines CCL27 and CCL28 are effective adjuvants in eliciting antigen-specific immunity in vivo.
Authors: Kutzler MA, Kraynyak KA, Nagle SJ, Parkinson RM, Zharikova D, Chattergoon M, Maguire H, Muthumani K, Ugen K, Weiner DB
Gene Ther., 2009-10-22;17(1):72-82.
Species: Mouse
Sample Types: Cell Lysates
Applications: Immunoprecipitation
Cutting edge: rapid accumulation of epidermal CCL27 in skin-draining lymph nodes following topical application of a contact sensitizer recruits CCR10-expressing T cells.
Authors: Huang V, Lonsdorf AS, Fang L, Kakinuma T, Lee VC, Cha E, Zhang H, Nagao K, Zaleska M, Olszewski WL, Hwang ST
J. Immunol., 2008-05-15;180(10):6462-6.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr