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Mouse CCL20/MIP-3 alpha Biotinylated Antibody

Catalog #: BAF760
5 Citations Datasheet
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BAF760
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Citations (5)
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Mouse CCL20/MIP-3 alpha Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse MIP-3 alpha  in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) MIP-3 alpha, rhMIP-3 beta, recombinant mouse (rm) MIP-3 beta, recombinant rat MIP-3 alpha, rmMIP-1 gamma, rmMIP-1 alpha, rmMIP-1 beta, and rmMIP-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CCL20/MIP-3 alpha (R&D Systems, Catalog # 760-M3)
Ala27-Met96
Accession # Q642U4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CCL20/MIP‑3 alpha (Catalog # 760-M3)

Mouse CCL20/MIP-3 alpha Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse CCL20/MIP‑3 alpha Antibody (Catalog # MAB7601)

Standard: Recombinant Mouse CCL20/MIP-3 alpha Protein (Catalog # 760-M3)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL20/MIP-3 alpha

MIP-3 alpha, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. Mouse MIP-3 alpha cDNA encodes a 97 amino acid residue precursor protein with a 27 aa residue putative signal peptide that is predicted to be cleaved to form the 70 aa residue mature secreted protein. MIP-3 alpha is distantly related to other beta chemokines (20 - 28% aa sequence identity). Mouse MIP-3 alpha shares approximately 71 and 63% amino acid sequence homology with rat and human MIP-3 alpha, respectively.

MIP-3 alpha has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung, and epithelial cells of intestinal tissues. The expression of MIP-3 alpha is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant MIP-3 alpha has been shown to be chemotactic for lymphocytes and dendritic cells, and inhibits proliferation of myeloid progenitors in colony formation assays. MIP-3 alpha has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.

References
  1. Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
  2. Hromas, R. et al. (1997) Blood 89:3315.
  3. Greaves, D. R. et al. (1997) J. Exp. Med. 186: 857.
  4. Tanaka, Y. et al. (1999) Eur. J. Immunol. 29:633.
Entrez Gene IDs
6364 (Human); 20297 (Mouse); 29538 (Rat)
Alternate Names
beta chemokine exodus-1; Beta-chemokine exodus-1; CC chemokine LARC; C-C motif chemokine 20; CCL20; chemokine (C-C motif) ligand 20; CKb4; exodus-1; LARC; LARCLiver and activation-regulated chemokine; MIP3 alpha; MIP-3 alpha; MIP-3a; MIP-3-alpha; MIP3AMacrophage inflammatory protein 3 alpha; SCYA20Small-inducible cytokine A20; small inducible cytokine subfamily A (Cys-Cys), member 20; ST38

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Citations for Mouse CCL20/MIP-3 alpha Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Regulation of inflammatory factors by double-stranded RNA receptors in breast cancer cells
    Authors: A Venkatesh, H Nandigam, M Muccioli, M Singh, T Loftus, D Lewis, M Pate, F Benencia
    Immunobiology, 2017-11-22;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  2. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
    Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
    Cell. Physiol. Biochem., 2017-08-09;42(5):1961-1972.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development (Detection)
  3. The chemokine receptor CCR6 facilitates the onset of mammary neoplasia in the MMTV-PyMT mouse model via recruitment of tumor-promoting macrophages.
    Authors: Boyle S, Faulkner J, McColl S, Kochetkova M
    Mol Cancer, 2015-06-06;14(0):115.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Detection)
  4. Colonic patch and colonic SILT development are independent and differentially regulated events.
    Authors: Baptista A, Olivier B, Goverse G, Greuter M, Knippenberg M, Kusser K, Domingues R, Veiga-Fernandes H, Luster A, Lugering A, Randall T, Cupedo T, Mebius R
    Mucosal Immunol, 2012-09-19;6(3):511-21.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Tumor-specific Th17-polarized cells eradicate large established melanoma.
    Authors: Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP
    Blood, 2008-03-19;112(2):362-73.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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