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Mouse CCL20/MIP-3 alpha Antibody

Catalog #: MAB760
7 Citations 1 Review Datasheet
Catalog # Availability Size / Price Qty
MAB760-SP
MAB760-100
MAB760-500
Detection of Mouse CCL20 by Western Blot.
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Product Details
Citations (7)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCL20/MIP-3 alpha Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL20/MIP‑3 alpha in direct ELISAs and Western blots. In direct ELISAs, 12% cross-reactivity with recombinant rat (rr) CCL20, 6% cross-reactivity with recombinant mouse (rm) CCL12 and recombinant human (rh) CCL25, and 3% cross‑reactivity with with rmCCL9/10/MIP-1 gamma is observed. No cross-reactivity with recombinant cotton rat CCL3, 4, 5, rhCCL1, 2, 3, 4, 5, 7, 8, 11,13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 26, 27, 28, rmCCL1, 2, 3, 4, 5, 6, 7, 8, 11, 17, 19, 21, 22, 24, 25, 27, or 28 is observed.
Source
Monoclonal Rat IgG1 Clone # 114906
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse CCL20/MIP-3 alpha
Ala28-Met97
Accession # O89093
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse CCL20 antibody by Western Blot. View Larger

Detection of Mouse CCL20 by Western Blot. Western blot shows lysates of mouse small intestine. PVDF membrane was probed with 2 µg/mL of Mouse CCL20/MIP-3a Monoclonal Antibody (Catalog # MAB760) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CCL20 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Mouse CCL20/MIP-3 alpha by Western Blot View Larger

Detection of Mouse CCL20/MIP-3 alpha by Western Blot Conditional macrophage ablation disrupted Treg-cell recruitment and inhibited the growth of CRC in mice.(A, B) Flow cytometric analysis of TAMs derived from grafted CRC in CD11b-DTR mice injected with DTmu or DT. (C) CCL20 mRNA levels in grafted CRC in CD11b-DTR mice injected with DTmu or DT (data from 5 mice of each group were pooled together). (D) Lysates of grafted CRC from CD11b-DTR mice injected with DTmu or DT were subjected to western blotting for CCL20 expression. Actin - loading control. (E) Immunofluorescence staining with anti-mouse CCL20 was performed on cryosections of GFP+ CMT93 grafted tumor mass derived from CD11b-DTR mice injected with DTmu or DT for 14 days. (F) Flow cytometric analysis of CCR6 expression for GFP+ CMT93 cells before grafting (left panel), and CCR6 staining on cryosections of GFP+ CMT93 grafted tumor mass derived from CD11b-DTR mice injected with DTmu or DT for 14 days (middle and right panel). (G, H) Number of tumor-infiltrating FoxP3+ Treg-cells in grafted CRC in CD11b-DTR mice injected with DTmu or DT. (I) CRC grafted in CD11b-DTR mice injected with DTmu or DT for 14 days. (J) Tumor weight of CRC grafted in CD11b-DTR mice injected with DTmu or DT for 14 days. Representative data are shown which had been reproduced in 2 independent experiments. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0019495), licensed under a CC-BY license. Not internally tested by R&D Systems.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL20/MIP-3 alpha

CCL20, also known as MIP-3 alpha, LARC, and Exodus, is a beta chemokine. Mouse CCL20 cDNA encodes a 97 amino acid precursor protein with a 27 aa putative signal peptide that is predicted to be cleaved to form the 70 aa mature secreted protein. CCL20 is distantly related to other beta chemokines (20‑28% aa sequence identity). Mouse CCL20 shares approximately 71 and 63% amino acid sequence homology with rat and human CCL20, respectively. CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung, and epithelial cells of intestinal tissues. The expression of CCL20 is strongly upregulated by inflammatory signals and downregulated by the anti-inflammatory cytokine IL-10. CCL20 has been shown to be chemotactic for lymphocytes and dendritic cells, and inhibits proliferation of myeloid progenitors in colony formation assays. CCL20 is a functional ligand for CCR6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.

References
  1. Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
  2. Hromas, R. et al. (1997) Blood 89:3315.
  3. Greaves, D. R. et al. (1997) J. Exp. Med. 186: 857.
  4. Tanaka, Y. et al. (1999) Eur. J. Immunol. 29:633.
Entrez Gene IDs
6364 (Human); 20297 (Mouse); 29538 (Rat)
Alternate Names
beta chemokine exodus-1; Beta-chemokine exodus-1; CC chemokine LARC; C-C motif chemokine 20; CCL20; chemokine (C-C motif) ligand 20; CKb4; exodus-1; LARC; LARCLiver and activation-regulated chemokine; MIP3 alpha; MIP-3 alpha; MIP-3a; MIP-3-alpha; MIP3AMacrophage inflammatory protein 3 alpha; SCYA20Small-inducible cytokine A20; small inducible cytokine subfamily A (Cys-Cys), member 20; ST38

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Citations for Mouse CCL20/MIP-3 alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. V&gamma4 T cell-derived IL-17A is essential for amplification of inflammatory cascades in ischemic brain tissue after stroke
    Authors: L Lu, Y Wang, L Zhou, Y Li, X Zhang, X Hu, S Shi, W He
    International immunopharmacology, 2021-05-24;96(0):107678.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  2. CCL20-CCR6 axis modulated traumatic brain injury-induced visual pathologies
    Authors: M Das, X Tang, JY Han, K Mayilsamy, E Foran, MR Biswal, R Tzekov, SS Mohapatra, S Mohapatra
    J Neuroinflammation, 2019-05-31;16(1):115.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  3. Role of S1P/S1PR3 axis in release of CCL20 from human bronchial epithelial cells
    Authors: Y Kawa, T Nagano, A Yoshizaki, R Dokuni, M Katsurada, T Terashita, Y Yasuda, K Umezawa, M Yamamoto, H Kamiryo, K Kobayashi, Y Nishimura
    PLoS ONE, 2018-09-07;13(9):e0203211.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  4. Mutations in BALB mitochondrial DNA induce CCL20 up-regulation promoting tumorigenic phenotypes
    Authors: James Sligh, Jaroslav Janda, Jana Jandova
    Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis
  5. Leukocyte attraction by CCL20 and its receptor CCR6 in humans and mice with pneumococcal meningitis.
    Authors: Klein M, Brouwer M, Angele B, Geldhoff M, Marquez G, Varona R, Hacker G, Schmetzer H, Hacker H, Hammerschmidt S, van der Ende A, Pfister H, van de Beek D, Koedel U
    PLoS ONE, 2014-04-03;9(4):e93057.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  6. Near-infrared laser adjuvant for influenza vaccine.
    Authors: Kashiwagi, Satoshi, Yuan, Jianping, Forbes, Benjamin, Hibert, Mathew L, Lee, Eugene L, Whicher, Laura, Goudie, Calum, Yang, Yuan, Chen, Tao, Edelblute, Beth, Collette, Brian, Edington, Laurel, Trussler, James, Nezivar, Jean, Leblanc, Pierre, Bronson, Roderick, Tsukada, Kosuke, Suematsu, Makoto, Dover, Jeffrey, Brauns, Timothy, Gelfand, Jeffrey, Poznansky, Mark C
    PLoS ONE, 2013-12-11;8(12):e82899.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Contribution of IL-17-producing gamma delta T cells to the efficacy of anticancer chemotherapy.
    Authors: Ma Y, Aymeric L, Locher C, Mattarollo SR, Delahaye NF, Pereira P, Boucontet L, Apetoh L, Ghiringhelli F, Casares N, Lasarte JJ, Matsuzaki G, Ikuta K, Ryffel B, Benlagha K, Tesniere A, Ibrahim N, Dechanet-Merville J, Chaput N, Smyth MJ, Kroemer G, Zitvogel L
    J. Exp. Med., 2011-03-07;208(3):491-503.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization

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Mouse CCL20/MIP-3 alpha Antibody
By Anonymous on 02/03/2022
Application: WB Sample Tested: Small intestine tissue Species: Mouse
Western Blot Mouse CCL20/MIP-3 alpha Antibody MAB760