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Mouse CCL12/MCP-5 Antibody

Catalog #: AF428
4 Citations Datasheet
Catalog # Availability Size / Price Qty
AF428
AF428-SP
Chemotaxis Induced by CCL12/MCP‑5 and Neutralization by Mouse CCL12/MCP‑5 Antibody.
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Citations (4)
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Mouse CCL12/MCP-5 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL12/MCP-5 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) MCP‑3 is observed and 5% cross-reactivity with recombinant mouse (rm) JE, rmMARC, rhMCP‑1, and rhMCP‑2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CCL12/MCP-5 (R&D Systems, Catalog # 428-P5)
Gly23-Gly104
Accession # Q62401
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CCL12/MCP‑5 (Catalog # 428-P5)
Neutralization
Measured by its ability to neutralize CCL12/MCP‑5-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.8-4.0 µg/mL in the presence of 0.2 µg/mL Recombinant Mouse CCL12/MCP‑5.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL12/MCP‑5 and Neutralization by Mouse CCL12/MCP‑5 Antibody. View Larger

Chemotaxis Induced by CCL12/MCP‑5 and Neutralization by Mouse CCL12/MCP‑5 Antibody. Recombinant Mouse CCL12/MCP‑5 (Catalog # 428-P5) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL12/MCP‑5 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL12/MCP‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF428). The ND50 is typically 0.8-4.0 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL12/MCP-5

CCL12 is a cloned mouse CC chemokine most closely related to human MCP-1 (66% amino acid (aa) sequence identity in the mature protein). Mouse CCL12 encodes a 104 aa residue precursor protein with a 22 aa residue predicted hydrophobic signal sequence that is cleaved to generate a 82 aa residue mature protein. CCL12 is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, CCL12 expression is also induced in activated macrophages and mast cells. At high concentrations, CCL12 will also chemoattract eosinophils. CCL12 has been found to be a functional ligand for CCR2 but not CCR1, CCR3, or CCR5.

References
  1. Jia, G.Q. et al. (1996) J. Exp. Med. 184:1939.
  2. Sarafi, M.N. et al. (1997) J. Exp. Med. 185:99.
Entrez Gene IDs
20293 (Mouse)
Alternate Names
C-C motif chemokine 12; CCL12; chemokine (C-C motif) ligand 12; MCP5; MCP-5; Scya12

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Citations for Mouse CCL12/MCP-5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Subventricular zone/white matter microglia reconstitute the empty adult microglial niche in a dynamic wave
    Authors: Lindsay A Hohsfield, Allison R Najafi, Yasamine Ghorbanian, Neelakshi Soni, Joshua Crapser, Dario X Figueroa Velez et al.
    eLife
  2. Transcriptional and Distributional Profiling of Microglia in Retinal Angiomatous Proliferation
    Authors: A Schlecht, J Wolf, S Boneva, G Prinz, BM Braunger, P Wieghofer, H Agostini, G Schlunck, C Lange
    International Journal of Molecular Sciences, 2022-03-22;23(7):.
    Species: Mouse
    Sample Types: In Vivo
  3. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010-02-08;120(3):907-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. The CCL7-CCL2-CCR2 axis regulates IL-4 production in lungs and fungal immunity.
    Authors: Szymczak WA, Deepe GS
    J. Immunol., 2009-07-08;183(3):1964-74.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization

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