Mouse CCL12/MCP-5 Antibody

Catalog #: MAB4281 Datasheet

Catalog #	Availability	Size / Price	Qty
MAB4281
MAB4281-SP

Chemotaxis Induced by CCL12/MCP‑5 and Neutral-ization by Mouse CCL12/ MCP‑5 Antibody.

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All CCL12/MCP-5 Antibodies

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Mouse CCL12/MCP-5 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCL12/MCP‑5 in direct ELISAs.

Source

Monoclonal Rat IgG_2B Clone # 147801

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse CCL12/MCP‑5
Gly23-Gly104
Accession # Q62401

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize CCL12/MCP‑5-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND₅₀) is typically 0.5-2.5 µg/mL in the presence of 0.2 µg/mL Recombinant Mouse CCL12/MCP‑5.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by CCL12/MCP‑5 and Neutral-ization by Mouse CCL12/ MCP‑5 Antibody. Recombinant Mouse CCL12/MCP-5 (Catalog # 428-P5) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL12/MCP-5 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL12/MCP-5 Monoclonal Antibody (Catalog # MAB4281). The ND₅₀ is typically 0.5-2.5 µg/mL.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL12/MCP-5

CCL12, also called MCP-5, is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, CCL12 expression is also induced in activated macrophages and mast cells. Mouse CCL12 encodes a 104 amino acid (aa) precursor protein with a 22 aa signal sequence that is cleaved to generate a 82 aa mature protein. Recombinant CCL12 is a potent chemoattractant for monocytes and lymphocytes but not neutrophils. CCL12 has been found to be a functional ligand for CCR2.

Entrez Gene IDs

20293 (Mouse)

Alternate Names

C-C motif chemokine 12; CCL12; chemokine (C-C motif) ligand 12; MCP5; MCP-5; Scya12