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Mouse CCL1/I-309/TCA-3 Antibody

Catalog #: MAB845
7 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB845-SP
MAB845-100
MAB845-500
Chemotaxis Induced by CCL1/ITCA‑3 and Neutralization by Mouse CCL1/ITCA‑3 Antibody.
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Citations (7)
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Mouse CCL1/I-309/TCA-3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL1 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant cotton rat CCL3, 4, 5, recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 23, 24, 25, 26, 27, 28, recombinant mouse CCL2, 3, 4,5,6, 7, 8, 9/10/MIP-1 gamma, 11, 12, 17, 19, 20, 21, 22, 24, 25, 27, 28, or recombinant rat CCL20.
Source
Monoclonal Rat IgG2A Clone # 148113
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CCL1/I-309/TCA-3
Lys24-Cys92
Accession # P10146
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse CCL1/I‑309/TCA‑3 (Catalog # 845-TC)
Neutralization
Measured by its ability to neutralize CCL1/I‑309/TCA‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR8. The Neutralization Dose (ND50) is typically 2-10 µg/mL in the presence of 0.01 µg/mL Recombinant Mouse CCL1/I‑309/TCA‑3.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL1/ITCA‑3 and Neutralization by Mouse CCL1/ITCA‑3 Antibody. View Larger

Chemotaxis Induced by CCL1/ITCA‑3 and Neutralization by Mouse CCL1/ITCA‑3 Antibody. Recombinant Mouse CCL1/TCA‑3 (Catalog # 845-TC) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL1/TCA‑3 (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL1/TCA‑3 Monoclonal Antibody (Catalog # MAB845). The ND50 is typically 2-10 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL1/I-309/TCA-3

Mouse CCL1, also known as TCA-3, is a member of the CC beta family of chemokines. The human chemokine I-309, which shares approximately 42% amino acid (aa) sequence identity, has been assumed to be the homologue of mouse TCA-3. Mouse TCA-3 and human I-309 also share significant sequence homology in the 5' flanking region of their genes and each contain an extra pair of cysteine residues not found in most other chemokines.

CCL1 cDNA encodes a 92 aa residue precursor protein with a predicted 23 aa signal peptide that is cleaved to produce a 69 aa mature protein. The sequence of CCL1 contains one potential N-linked glycosylation site. Mouse CCL1 is found on the distal portion of mouse chromosome 11 in a cluster with MIP-1 alpha, MIP-1 beta and JE. CCL1 acts by binding to the seven transmembrane spanning G-protein-coupled receptor, CCR8. CCL1 has been shown to chemoattract T cells.

References
  1. Goya, I. et al. (1998) J. Immunol. 160:1975.
  2. Burd, P. et al. (1987) J. Immunol. 139:3126.
Entrez Gene IDs
6346 (Human); 20290 (Mouse)
Alternate Names
C-C motif chemokine 1; CCL1; chemokine (C-C motif) ligand 1; I-309; inflammatory cytokine I-309; P500; SCYA1; SCYA1Small-inducible cytokine A1; SCYA2; SISe; small inducible cytokine A1 (I-309, homologous to mouse Tca-3); T lymphocyte-secreted protein I-309; TCA3; TCA-3

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Citations for Mouse CCL1/I-309/TCA-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Gut-derived memory ?? T17 cells exacerbate sepsis-induced acute lung injury in mice
    Authors: Xie, B;Wang, M;Zhang, X;Zhang, Y;Qi, H;Liu, H;Wu, Y;Wen, X;Chen, X;Han, M;Xu, D;Sun, X;Zhang, X;Zhao, X;Shang, Y;Yuan, S;Zhang, J;
    Nature communications
    Species: Mouse
    Sample Types: Cell Lysates, Tissue Homogenates, In Vivo
    Applications: Western Blot, In vivo assay
  2. Cytotoxic T cells swarm by homotypic chemokine signalling
    Authors: Jorge Luis Galeano Niño, Sophie V Pageon, Szun S Tay, Feyza Colakoglu, Daryan Kempe, Jack Hywood et al.
    eLife
  3. Astroglial TLR9 antagonism promotes chemotaxis and alternative activation of macrophages via modulation of astrocyte-derived signals: implications for spinal cord injury
    Authors: L Li, L Ni, RF Heary, S Elkabes
    J Neuroinflammation, 2020-02-25;17(1):73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  4. The Early Activation Marker CD69 Regulates the Expression of Chemokines and CD4 T Cell Accumulation in Intestine
    Authors: Katarina Radulovic, Valerio Rossini, Calin Manta, Karlheinz Holzmann, Hans A. Kestler, Jan Hendrik Niess
    PLoS ONE
  5. Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells.
    Authors: Islam SA, Chang DS, Colvin RA
    Nat. Immunol., 2011-01-09;12(2):167-77.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  6. Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway.
    Authors: Kearley J, Buckland KF, Mathie SA, Lloyd CM
    Am. J. Respir. Crit. Care Med., 2009-01-29;179(9):772-81.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  7. Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions.
    Authors: Mosmann T
    Eur. J. Immunol., 2004-06-01;34(6):1617-26.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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