Mouse Bcl-2 Minus C-Terminus Antibody

Catalog #: MAB810
1 Citation Datasheet

Catalog #	Availability	Size / Price	Qty
MAB810
MAB810-SP

Detection of Mouse Bcl‑2 by Western Blot.

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All Bcl-2 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Bcl-2 Minus C-Terminus Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Bcl‑2 Minus C-Terminus in Western blots.

Source

Monoclonal Rat IgG_2B Clone # 121529

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse Bcl-2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Bcl‑2 by Western Blot. Western blot shows lysates of CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 0.5 µg/mL Rat Anti-Mouse Bcl-2 Minus C-Terminus Monoclonal Antibody (Catalog # MAB810) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, Recombinant Human Bcl-2 Minus C-Terminus (Catalog # 827-BC) (2 ng) was included. A specific band for Bcl-2 was detected at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bcl-2

The Bcl-2 family of proteins plays a crucial role in the regulation of cell death in many eukaryotic systems. Bcl-2 is a 26 kD protein associated with cell survival. Over-expression of Bcl-2 has been shown to promote cell survival by suppressing apoptosis in a number of cells. The ratio of Bcl-2 to other Bcl-2 family members and subcellular localization are believed to modulate the apoptotic process.

Long Name

B Cell Lymphoma/Leukemia 2

Entrez Gene IDs

596 (Human); 12043 (Mouse); 24224 (Rat)

Alternate Names

apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; Bcl2; Bcl-2

Product Datasheets

Product Datasheet

Related Research Areas

Citation for Mouse Bcl-2 Minus C-Terminus Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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