Mouse Bad N-Terminus Antibody

Catalog #: MAB850
1 Citation 1 Review Datasheet

Catalog #	Availability	Size / Price	Qty
MAB850
MAB850-SP

Detection of Mouse Bad N-Terminus by Western Blot.

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Bad N-Terminus Antibody Summary

Species Reactivity

Mouse

Specificity

Detects endogenous mouse Bad in Western blots. In Western blots, this antibody does not cross-react with human Bad. The antibody also detects a 32 kDa band in Western blots.

Source

Monoclonal Rat IgG_2A Clone # 64103

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse Bad

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Bad N-Terminus by Western Blot. Western blot shows lysates of CTLL-2 mouse cytotoxic T cell line and L-929 mouse fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse Bad N-Terminus Monoclonal Antibody (Catalog # MAB850) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Bad N-Terminus at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry

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Bad in NIH‑3T3 Mouse Cell Line. Bad was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Rat Anti-Mouse Bad N-Terminus Monoclonal Antibody (Catalog # MAB850) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bad

Bcl-2 antagonist of cell death (Bad) is an 18 kDa cytoplasmic protein in the Bcl-2 family. It functions as a pro-apoptotic molecule by dimerizing with and inhibiting the anti-apoptotic proteins Bcl-2 and Bcl-xL. Prosurvival signals trigger the phosphorylation of Bad on Ser75, Ser99, and Ser115, disrupting its interaction with Bcl-2 and Bcl-xL and resulting in protection from apoptosis. Phosphorylation of Ser75 and Ser99 is also required for the ability of Bad to induce cell cycle arrrest in G1. Human Bad shares 75 % aa sequence identity with mouse and rat Bad.

Long Name

Bcl-xL/Bcl-2 Associated Death Promoter

Entrez Gene IDs

572 (Human); 12015 (Mouse); 64639 (Rat)

Alternate Names

Bad; BBC2; BBC6; bcl2 antagonist of cell death; BCL2-antagonist of cell death protein; BCL2-associated agonist of cell death; Bcl-2-binding component 6; BCL2-binding component 6; BCL2-binding protein; BCL2L8; Bcl2-L-8; BCL2L8bcl2-L-8; Bcl-2-like protein 8; BCL-X/BCL-2 binding protein; Bcl-XL/Bcl-2-associated death promoter

Product Datasheets

Product Datasheet

Related Research Areas

Citation for Mouse Bad N-Terminus Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Caspase-mediated Cleavage of Insulin Receptor Substrate.
Authors: Green KA, Naylor MJ, Lowe ET, Wang P, Marshman E, Streuli CH
J. Biol. Chem., 2004-04-05;279(24):25149-56.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot