Mouse Axl Antibody Summary
His20-Pro443
Accession # Q80YQ3
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Axl in Mouse Kidney. Axl was detected in immersion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Mouse Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF854) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Axl
Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The mouse Axl cDNA encodes an 888 amino acid (aa) precursor that includes an 18 aa signal sequence, a 427 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of mouse and human Axl share 81% aa sequence identity. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.
- Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
- Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
- Holland, S. et al. (2005) Canc. Res. 65:9294.
Product Datasheets
Citations for Mouse Axl Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Targeting inflammation in type 2 diabetes by antibody‐mediated Tyro‐3, Axl, Mer receptor activation
Authors: Chien‐Hsing Lee, Chih‐Ying Changchien, Yi‐Jen Hung
Journal of Diabetes Investigation
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Sensing of apoptotic cells through Axl causes lung basal cell proliferation in inflammatory diseases
Authors: Naoya Fujino, Oliver J. Brand, David J. Morgan, Toshifumi Fujimori, Aleksander M. Grabiec, Christopher P. Jagger et al.
Journal of Experimental Medicine
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Deficiency of Axl aggravates pulmonary arterial hypertension via BMPR2
Authors: Tatyana Novoyatleva, Nabham Rai, Baktybek Kojonazarov, Swathi Veeroju, Isabel Ben-Batalla, Paola Caruso et al.
Communications Biology
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Subventricular zone/white matter microglia reconstitute the empty adult microglial niche in a dynamic wave
Authors: Lindsay A Hohsfield, Allison R Najafi, Yasamine Ghorbanian, Neelakshi Soni, Joshua Crapser, Dario X Figueroa Velez et al.
eLife
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The TAM-family receptor Mer mediates production of HGF through the RhoA-dependent pathway in response to apoptotic cells
Authors: Hyun-Jung Park, Ji-Yeon Baen, Ye-Ji Lee, Youn-Hee Choi, Jihee Lee Kang
Molecular Biology of the Cell
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Differential regulation of hepatic physiology and injury by the TAM receptors Axl and Mer
Authors: Anna Zagórska, Paqui G Través, Lidia Jiménez-García, Jenna D Strickland, Joanne Oh, Francisco J Tapia et al.
Life Science Alliance
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Enhanced efficacy of sitravatinib in metastatic models of antiangiogenic therapy resistance
Authors: Melissa Dolan, Michalis Mastri, Amanda Tracz, James G. Christensen, Gurkamal Chatta, John M. L. Ebos
PLOS ONE
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MerTK inhibition in tumor leukocytes decreases tumor growth and metastasis
Authors: Rebecca S. Cook, Kristen M. Jacobsen, Anne M. Wofford, Deborah DeRyckere, Jamie Stanford, Anne L. Prieto et al.
Journal of Clinical Investigation
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AXL receptor tyrosine kinase is required for T cell priming and antiviral immunity.
Authors: Schmid ET, Pang IK, Carrera Silva EA et al.
Elife
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Axl-mediated activation of TBK1 drives epithelial plasticity in pancreatic cancer
Authors: Victoria H. Cruz, Emily N. Arner, Wenting Du, Alberto E. Bremauntz, Rolf A. Brekken
JCI Insight
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Concerted type I interferon signaling in microglia and neural cells promotes memory impairment associated with amyloid ? plaques
Authors: Roy ER, Chiu G, Li S et al.
Immunity
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TAM Receptors Are Not Required for Zika Virus Infection in Mice
Authors: AK Hastings, LJ Yockey, BW Jagger, J Hwang, R Uraki, HF Gaitsch, LA Parnell, B Cao, IU Mysorekar, CV Rothlin, E Fikrig, MS Diamond, A Iwasaki
Cell Rep, 2017-04-18;19(3):558-568.
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AXL Expression on Homeostatic Resident Liver Macrophages Is Reduced in Cirrhosis Following GAS6 Production by Hepatic Stellate Cells
Authors: Oltin-Tiberiu Pop, Anne Geng, Emilio Flint, Arjuna Singanayagam, Caner Ercan, Lucia Possamai et al.
Cellular and Molecular Gastroenterology and Hepatology
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Rapid unleashing of macrophage efferocytic capacity via transcriptional pause release
Authors: Tufan, T;Comertpay, G;Villani, A;Nelson, GM;Terekhova, M;Kelley, S;Zakharov, P;Ellison, RM;Shpynov, O;Raymond, M;Sun, J;Chen, Y;Bockelmann, E;Stremska, M;Peterson, LW;Boeckaerts, L;Goldman, SR;Etchegaray, JI;Artyomov, MN;Peri, F;Ravichandran, KS;
Nature
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Depletion of slow-cycling PDGFR?+ADAM12+ mesenchymal cells promotes antitumor immunity by restricting macrophage efferocytosis
Authors: Di Carlo, SE;Raffenne, J;Varet, H;Ode, A;Granados, DC;Stein, M;Legendre, R;Tuckermann, J;Bousquet, C;Peduto, L;
Nature immunology
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Transcriptomic analysis reveals partial epithelial-mesenchymal transition and inflammation as common pathogenic mechanisms in hypertensive nephrosclerosis and Type 2 diabetic nephropathy
Authors: Nordbø, OP;Landolt, L;Eikrem, Ø;Scherer, A;Leh, S;Furriol, J;Apeland, T;Mydel, P;Marti, HP;
Physiological reports
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
LZTR1 mutation mediates oncogenesis through stabilization of EGFR and AXL
Authors: A Ko, M Hasanain, YT Oh, F D'Angelo, D Sommer, B Frangaj, S Tran, F Bielle, B Pollo, R Paterra, K Mokhtari, RK Soni, M Peyre, M Eoli, L Papi, M Kalamaride, M Sanson, A Iavarone, A Lasorella
Cancer Discovery, 2023-03-01;0(0):.
Species: Transgenic Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot -
A TREM2-activating antibody with a blood-brain barrier transport vehicle enhances microglial metabolism in Alzheimer's disease models
Authors: B van Lenger, L Zhan, D Xia, D Chan, D Joy, JI Park, D Tatarakis, M Calvert, S Hummel, S Lianoglou, ME Pizzo, R Prorok, E Thomsen, LM Bartos, P Beumers, A Capell, SS Davis, L de Weerd, JC Dugas, J Duque, T Earr, K Gadkar, T Giese, A Gill, J Gnörich, C Ha, M Kannuswamy, DJ Kim, ST Kunte, LH Kunze, D Lac, K Lechtenber, AW Leung, CC Liang, I Lopez, P McQuade, A Modi, VO Torres, HN Nguyen, I Pesämaa, N Propson, M Reich, Y Robles-Col, K Schlepckow, L Slemann, H Solanoy, JH Suh, RG Thorne, C Vieira, K Wind-Mark, K Xiong, YJY Zuchero, D Diaz, MS Dennis, F Huang, K Scearce-Le, RJ Watts, C Haass, JW Lewcock, G Di Paolo, M Brendel, PE Sanchez, KM Monroe
Nature Neuroscience, 2023-01-12;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Inhibitory effect of the novel tyrosine kinase inhibitor DCC-2036 on triple-negative breast cancer stem cells through AXL-KLF5 positive feedback loop
Authors: Y Shen, Q Zhu, M Xiao, L Yin, W Feng, J Feng, J He, P Li, X Chen, W Ding, J Zhong, Z Zeng, Z Xie, J Liu, X Zu
Cell Death & Disease, 2022-08-30;13(8):749.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Anti-inflammatory clearance of amyloid-beta by a chimeric Gas6 fusion protein
Authors: H Jung, SY Lee, S Lim, HR Choi, Y Choi, M Kim, S Kim, Y Lee, KH Han, WS Chung, CH Kim
Nature Medicine, 2022-08-04;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Stromal changes in the aged lung induce an emergence from melanoma dormancy
Authors: ME Fane, Y Chhabra, GM Alicea, DA Maranto, SM Douglass, MR Webster, VW Rebecca, GE Marino, F Almeida, BL Ecker, DJ Zabransky, L Hüser, T Beer, HY Tang, A Kossenkov, M Herlyn, DW Speicher, W Xu, X Xu, EM Jaffee, JA Aguirre-Gh, AT Weeraratna
Nature, 2022-06-01;606(7913):396-405.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Concerted type I interferon signaling in microglia and neural cells promotes memory impairment associated with amyloid ? plaques
Authors: Roy ER, Chiu G, Li S et al.
Immunity
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Ligand-dependent kinase activity of MERTK drives efferocytosis in human iPSC-derived macrophages
Authors: F Wanke, S Gutbier, A Rümmelin, M Steinberg, LD Hughes, M Koenen, J Komuczki, D Regan-Komi, S Wagage, J Hesselmann, R Thoma, D Brugger, T Christopei, H Wang, F Point, R Hallet, S Ghosh, CV Rothlin, C Patsch, B Geering
Cell Death & Disease, 2021-05-25;12(6):538.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Microglia use TAM receptors to detect and engulf amyloid &beta plaques
Authors: Y Huang, KE Happonen, PG Burrola, C O'Connor, N Hah, L Huang, A Nimmerjahn, G Lemke
Nature Immunology, 2021-04-15;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Embryonic Microglia Interact with Hypothalamic Radial Glia during Development and Upregulate the TAM Receptors MERTK and AXL following an Insult
Authors: JM Rosin, CM Marsters, F Malik, R Far, L Adnani, C Schuurmans, QJ Pittman, DM Kurrasch
Cell Reports, 2021-01-05;34(1):108587.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Essential omega-3 fatty acids tune microglial phagocytosis of synaptic elements in the mouse developing brain
Authors: C Madore, Q Leyrolle, L Morel, M Rossitto, AD Greenhalgh, JC Delpech, M Martinat, C Bosch-Bouj, J Bourel, B Rani, C Lacabanne, A Thomazeau, KE Hopperton, S Beccari, A Sere, A Aubert, V De Smedt-P, C Lecours, K Bisht, L Fourgeaud, S Gregoire, L Bretillon, N Acar, NJ Grant, J Badaut, P Gressens, A Sierra, O Butovsky, ME Tremblay, RP Bazinet, C Joffre, A Nadjar, S Layé
Nature Communications, 2020-11-30;11(1):6133.
Species: Mouse
Sample Types: Protein
Applications: Western Blot -
Anti-Axl antibody treatment reduces the severity of experimental autoimmune encephalomyelitis
Authors: JC DuBois, AK Ray, P Davies, B Shafit-Zag
J Neuroinflammation, 2020-10-29;17(1):324.
Species: Mouse
Sample Types: In Vivo
Applications: Functional Assay -
Effects of long-term and brain-wide colonization of peripheral bone marrow-derived myeloid cells in the CNS
Authors: LA Hohsfield, AR Najafi, Y Ghorbanian, N Soni, EE Hingco, SJ Kim, AD Jue, V Swarup, MA Inlay, KN Green
Journal of Neuroinflammation, 2020-09-20;17(1):279.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
YAP1/TAZ drives ependymoma-like tumour formation in mice
Authors: N Eder, F Roncaroli, MC Dolmart, S Horswell, F Andreiuolo, HR Flynn, AT Lopes, S Claxton, JP Kilday, L Collinson, JH Mao, T Pietsch, B Thompson, AP Snijders, SK Ultanir
Nat Commun, 2020-05-13;11(1):2380.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
A FUNCTIONAL ROLE OF GAS6/TAM IN NON-ALCOHOLIC STEATOHEPATITIS PROGRESSION IMPLICATES AXL AS THERAPEUTIC TARGET
Authors: A Tutusaus, E de Gregori, B Cucarull, H Cristóbal, C Aresté, I Graupera, M Coll, A Colell, G Gausdal, JB Lorens, P García de, A Morales, M Marí
Cell Mol Gastroenterol Hepatol, 2019-11-02;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture -
AXL targeting reduces fibrosis development in experimental unilateral ureteral obstruction
Authors: L Landolt, J Furriol, J Babickova, L Ahmed, Ø Eikrem, T Skogstrand, A Scherer, S Suliman, S Leh, JB Lorens, G Gausdal, HP Marti, T Osman
Physiol Rep, 2019-05-01;7(10):e14091.
Species: Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot -
Epigenetic regulation of brain region-specific microglia clearance activity
Authors: P Ayata, A Badimon, HJ Strasburge, MK Duff, SE Montgomery, YE Loh, A Ebert, AA Pimenova, BR Ramirez, AT Chan, JM Sullivan, I Purushotha, JR Scarpa, AM Goate, M Busslinger, L Shen, B Losic, A Schaefer
Nat. Neurosci., 2018-07-23;21(8):1049-1060.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Schwann cells use TAM receptor-mediated phagocytosis in addition to autophagy to clear myelin in a mouse model of nerve injury
Authors: A Brosius Lu, WS Chung, SA Sloan, GA Carson, L Zhou, E Lovelett, S Posada, JB Zuchero, BA Barres
Proc. Natl. Acad. Sci. U.S.A., 2017-09-05;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
Mouse macrophages show different requirements for phosphatidylserine receptor Tim4 in efferocytosis
Authors: Y Yanagihash, K Segawa, R Maeda, YI Nabeshima, S Nagata
Proc. Natl. Acad. Sci. U.S.A., 2017-08-02;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
MicroRNA-34a dependent regulation of AXL controls the activation of dendritic cells in inflammatory arthritis
Authors: M Kurowska-S, S Alivernini, EG Melchor, A Elmesmari, B Tolusso, C Tange, L Petricca, DS Gilchrist, G Di Sante, C Keijzer, L Stewart, C Di Mario, V Morrison, JM Brewer, D Porter, S Milling, RD Baxter, D McCarey, E Gremese, G Lemke, G Ferracciol, C McSharry, IB McInnes
Nat Commun, 2017-06-22;8(0):15877.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Migrating Interneurons Secrete Fractalkine to Promote Oligodendrocyte Formation in the Developing Mammalian Brain
Authors: A Voronova, SA Yuzwa, BS Wang, S Zahr, C Syal, J Wang, DR Kaplan, FD Miller
Neuron, 2017-05-03;94(3):500-516.e9.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Axl is not an indispensable factor for Zika virus infection in mice
Authors: Wang ZY, Wang Z, Zhen ZD et al.
J. Gen. Virol.
Species: Mouse
Sample Types:
Applications: ICC/IF -
Opposing Roles of Tyrosine Kinase Receptors Mer and Axl Determine Clinical Outcomes in Experimental Immune-Mediated Nephritis
J Immunol, 2016-08-15;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
TAM receptors regulate multiple features of microglial physiology
Authors: L Fourgeaud, PG Través, Y Tufail, H Leal-Baile, ED Lew, PG Burrola, P Callaway, A Zagórska, CV Rothlin, A Nimmerjahn, G Lemke
Nature, 2016-04-06;532(7598):240-4.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Nuclear receptors license phagocytosis by trem2+ myeloid cells in mouse models of Alzheimer's disease.
Authors: Savage J, Jay T, Goduni E, Quigley C, Mariani M, Malm T, Ransohoff R, Lamb B, Landreth G
J Neurosci, 2015-04-22;35(16):6532-43.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Diversification of TAM receptor tyrosine kinase function.
Authors: Zagorska A, Traves P, Lew E, Dransfield I, Lemke G
Nat Immunol, 2014-09-07;15(10):920-8.
Species: Mouse
Sample Types: Cell Lysates, In Vivo
Applications: Immunoprecipitation, Neutralization -
Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis.
J. Exp. Med., 2012-10-15;209(11):2033-47.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Protein S protects neurons from excitotoxic injury by activating the TAM receptor Tyro3-phosphatidylinositol 3-kinase-Akt pathway through its sex hormone-binding globulin-like region.
Authors: Zhong Z, Wang Y, Guo H, Sagare A, Fernandez JA, Bell RD, Barrett TM, Griffin JH, Freeman RS, Zlokovic BV
J. Neurosci., 2010-11-17;30(46):15521-34.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Protein S controls hypoxic/ischemic blood-brain barrier disruption through the TAM receptor Tyro3 and sphingosine 1-phosphate receptor.
Authors: Zhu D, Wang Y, Singh I
Blood, 2010-03-26;115(23):4963-72.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Gas6 receptors Axl, Sky and Mer enhance platelet activation and regulate thrombotic responses.
Authors: Gould WR, Baxi SM, Schroeder R, Peng YW, Leadley RJ, Peterson JT, Perrin LA
J. Thromb. Haemost., 2005-02-23;3(4):733-41.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
MERTK Inhibition: Potential as a Treatment Strategy in EGFR Tyrosine Kinase Inhibitor-Resistant Non-Small Cell Lung Cancer
Authors: Chao-Ju Chen, Yu-Peng Liu
Pharmaceuticals (Basel)
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Diet-dependent regulation of TGF beta impairs reparative innate immune responses after demyelination
Authors: Mar Bosch-Queralt, Ludovico Cantuti-Castelvetri, Alkmini Damkou, Martina Schifferer, Kai Schlepckow, Ioannis Alexopoulos et al.
Nature Metabolism
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Single-cell CRISPR immune screens reveal immunological roles of tumor intrinsic factors
Authors: Jiakai Hou, Shaoheng Liang, Chunyu Xu, Yanjun Wei, Yunfei Wang, Yukun Tan et al.
NAR Cancer
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Adaptable toolbox to characterize Alzheimer’s disease pathology in mouse models
Authors: Youtong H, Greg L
STAR Protocols
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The TAM receptor Mertk protects against neuroinvasive viral infection by maintaining blood-brain barrier integrity
Authors: Jonathan J. Miner, Brian P. Daniels, Bimmi Shrestha, Jose L. Proenca-Modena, Erin D. Lew, Helen M. Lazear et al.
Nature Medicine
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Astrocytic phagocytosis is a compensatory mechanism for microglial dysfunction
Authors: Hiroyuki Konishi, Takayuki Okamoto, Yuichiro Hara, Okiru Komine, Hiromi Tamada, Mitsuyo Maeda et al.
The EMBO Journal
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AXL Inhibition in Macrophages Stimulates Host-versus-Leukemia Immunity and Eradicates Naïve and Treatment-Resistant Leukemia
Authors: Irene Tirado-Gonzalez, Arnaud Descot, Devona Soetopo, Aleksandra Nevmerzhitskaya, Alexander Schäffer, Ivan-Maximilano Kur et al.
Cancer Discovery
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Differential TAM receptor-ligand-phospholipid interactions delimit differential TAM bioactivities.
Authors: Lew ED, Oh J, Burrola PG et al.
Elife.
-
Yap/Taz promote the scavenging of extracellular nutrients through macropinocytosis
Authors: Bryan King, Jingwen Araki, Wilhelm Palm, Craig B. Thompson
Genes & Development
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Distinct tumor architectures for metastatic colonization of the brain
Authors: S Gan, DG Macalinao, SH Shahoei, L Tian, X Jin, H Basnet, JT Muller, P Atri, E Seffar, W Chatila, AK Hadjantona, N Schultz, E Brogi, TA Bale, D Pe'er, J Massagué
bioRxiv : the preprint server for biology, 2023-01-28;0(0):.
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Phosphatidylserine Exposure Controls Viral Innate Immune Responses by Microglia
Authors: Tufail Y, Cook D, Fourgeaud L et al.
Neuron
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