Home / Angiopoietin-like Protein 3/ANGPTL3 / Mouse Angiopoietin-like Protein 3/ANGPTL3 Antibody

Mouse Angiopoietin-like Protein 3/ANGPTL3 Antibody

Catalog #: AF136
9 Citations Datasheet
Catalog # Availability Size / Price Qty
AF136
AF136-SP
Detection of Mouse Angiopoietin‑like Protein 3/ANGPTL3 by Western Blot.
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Citations (9)
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Mouse Angiopoietin-like Protein 3/ANGPTL3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Angiopoietin-like Protein 3/ANGPTL3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) Angiopoietin-1, rhAngiopoietin-2, recombinant mouse Angiopoietin-3, rhAngiopoietin-4, and rhAngiopoietin-like factor/CDT6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Angiopoietin-like Protein 3/ANGPTL3
Ser17-Thr455
Accession # Q9R182
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Angiopoietin-like Protein 3/ANGPTL3 antibody by Western Blot. View Larger

Detection of Mouse Angiopoietin‑like Protein 3/ANGPTL3 by Western Blot. Western blot shows lysates of mouse liver tissue and Hepa 1-6 mouse hepatoma cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Angiopoietin-like Protein 3/ANGPTL3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF136) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Angiopoietin-like Protein 3/ANGPTL3 at approximately 35 kDa (major band) and 52 & 63 kDa (minor bands, as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Angiopoietin-like 3 antibody in Mouse Liver by Immunohistochemistry (IHC-Fr). View Larger

Angiopoietin-like 3 in Mouse Liver. Angiopoietin-like 3 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse Angiopoietin-like 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF136) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 3/ANGPTL3

ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins (1‑3). Mature mouse ANGPTL3 contains an N‑terminal coiled‑coil domain and a C‑terminal fibrinogen-like domain (4). ANGPTL3 is expressed in the liver from early in development through adulthood (4, 5). Full length ANGPTL3 circulates in the plasma as do the proteolytically separated N- and C‑terminal fragments containing the coiled-coil domain and fibrinogen-like domains, respectively (6, 7). ANGPTL3 is found as 70 kDa, 50 kDa, and 32 kDa species and can form weakly associated noncovalent multimers in vitro (5, 6). ANGPTL3 directly inhibits lipoprotein lipase (LPL), an enzyme responsible for hydrolyzing circulating triglycerides (8). This activity requires a putative heparin‑binding motif that is N‑terminal to the coiled‑coil domain (6). Proteolytic removal of the fibrinogen-like domain from the N-terminal fragment serves to activate ANGPTL3 and increase its ability to inhibit LPL in vitro and function in vivo (6). ANGPTL3 promotes an increase in circulating triglyceride levels without altering VLDL or HDL secretion or uptake (6‑8). ANGPTL3 knockout mice are hypolipidemic and have elevated LPL activity (9). ANGPTL3 expression in vivo is up‑regulated by LXR agonists and down‑regulated by insulin, leptin, and TR beta agonists (10‑12). Dysregulated ANGPTL3 expression and elevated plasma triglyceride levels are characteristic of some strains of obese and diabetic mice, (7, 8, 11). ANGPTL3 does not bind Tie-1 or Tie-2 but its fibrinogen-like domain interacts with integrin alpha V beta 3 to induce endothelial cell adhesion, migration, and neovascularization (13). ANGPTL3, secreted by fetal liver cells, also promotes the expansion of hematopoietic stem cells (14). Mature mouse ANGPTL3 shares 22%‑30% amino acid (aa) sequence identity with ANGPTL1, 2, 4, 6, and 7. It shares 77% aa sequence identity with human ANGPTL3.

References
  1. Li, C. (2006) Curr. Opin. Lipidol. 17:152.
  2. Oike, Y. et al. (2004) Int. J. Hematol. 80:21.
  3. Kersten, S. (2005) Biochem. Soc. Transact. 33:1059.
  4. Conklin, D. et al. (1999) Genomics 62:477.
  5. Ge, H. et al. (2005) J. Lipid Res. 46:1484.
  6. Ono, M. et al. (2003) J. Biol. Chem. 278:41804.
  7. Koishi, R. et al. (2002) Nat. Genet. 30:151.
  8. Shimizugawa, T. et al. (2002) J. Biol. Chem. 277:33742.
  9. Koster, A. et al. (2005) Endocrinology 146:4943.
  10. Inaba, T. et al. (2003) J. Biol. Chem. 278:21344.
  11. Shimamura, M. et al. (2004) Biochem. Biophys. Res. Commun. 322:1080.
  12. Fugier, C. et al. (2006) J. Biol. Chem. 281:11553.
  13. Camenisch, G. et al. (2002) J. Biol. Chem. 277:17281.
  14. Zhang, C.C. et al. (2006) Nat. Med. 12:240.
Entrez Gene IDs
27329 (Human); 30924 (Mouse); 102136264 (Cynomolgus Monkey)
Alternate Names
AGNPT5; Ang-5; angiopoietin 5; Angiopoietin-5; angiopoietin-like 3; Angiopoietin-like Protein 3; angiopoietin-related protein 3; ANGPT5angiopoietin-5; ANGPTL3; FHBL2

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Citations for Mouse Angiopoietin-like Protein 3/ANGPTL3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. ANGPTL8 has both endocrine and autocrine effects on substrate utilization
    Authors: Federico Oldoni, Haili Cheng, Serena Banfi, Viktoria Gusarova, Jonathan C. Cohen, Helen H. Hobbs
    JCI Insight
  2. Postprandial exercise regulates tissue-specific triglyceride uptake through angiopoietin-like proteins
    Authors: Liu, X;Zhang, Y;Han, B;Li, L;Li, Y;Ma, Y;Kang, S;Li, Q;Kong, L;Huang, K;Song, BL;Liu, Y;Wang, Y;
    JCI insight
    Species: Mouse, Rat
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: Western Blot
  3. Liver-specific in vivo base editing of Angptl3 via AAV delivery efficiently lowers blood lipid levels in mice
    Authors: Zuo, Y;Zhang, C;Zhou, Y;Li, H;Xiao, W;Herzog, RW;Xu, J;Zhang, J;Chen, YE;Han, R;
    Cell & bioscience
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Hepatic ER stress suppresses adipose browning through ATF4-CIRP-ANGPTL3 cascade
    Authors: S Lv, Y Zhou, J Chen, H Yuan, ZN Zhang, B Luan
    Cell Reports, 2022-09-27;40(13):111422.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  5. ANGPTL3 impacts proteinuria and hyperlipidemia in primary nephrotic syndrome
    Authors: F Zhong, S Liu, Y Li, G Li, M Liu, J Wang, W Cui, Y Suo, X Gao
    Lipids in Health and Disease, 2022-04-10;21(1):38.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. GALNT2 regulates ANGPTL3 cleavage in cells and in vivo of mice
    Authors: X Li, Y Zhang, M Zhang, Y Wang
    Sci Rep, 2020-09-30;10(1):16168.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  7. Furin is the primary in vivo convertase of angiopoietin-like 3 and endothelial lipase in hepatocytes.
    Authors: Essalmani R, Susan-Resiga D, Chamberland A, Asselin M, Canuel M, Constam D, Creemers J, Day R, Gauthier D, Prat A, Seidah N
    J Biol Chem, 2013-08-05;288(37):26410-8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Angiopoietin-like protein 3 regulates the motility and permeability of podocytes by altering nephrin expression in vitro.
    Authors: Gao X, Xu H, Liu H
    Biochem. Biophys. Res. Commun., 2010-07-13;399(1):31-6.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Fetal liver hepatic progenitors are supportive stromal cells for hematopoietic stem cells.
    Authors: Chou S, Lodish HF
    Proc. Natl. Acad. Sci. U.S.A., 2010-04-12;107(17):7799-804.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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