Mouse Angiopoietin-2 Antibody Summary
Asp68-Phe496
Accession # O35608
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse Angiopoietin‑2 by Western Blot. Western blot shows lysates of mouse placenta tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Mouse Angiopoietin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7186) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Angiopoietin-2 at approximately 60 to 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Angiopoietin-2
Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted 66-70 kDa glycoprotein member of the angiopoietin family of growth factors. It is expressed by endothelial cells, macrophages and skeletal muscle fibers, and binds to the Tie2 receptor on select cell types, including endothelial cells and monocytes. Ang-2 has multiple functions. In the presence of growth factors, it promotes angiogenesis; in the absence of growth factors, it induces vascular regression. It is generally considered to be an antagonist of Ang-1 activity. Mature mouse Ang-2 is 478 amino acids (aa) in length (aa 19-496). It contains one coiled-coil region (aa 159-256) plus a C-terminal Fibrinogen-like domain (aa 275-495). Mouse Ang-2 undergoes covalent oligomerization, forming 140 kDa homodimers and 280 kDa homotetramers. The higher-order oligomers are strong Tie-2 agonists. Over aa 68-496, mouse Ang-2 shares 87% and 96% aa sequence identity with human and rat Ang-2, respectively.
Product Datasheets
Citations for Mouse Angiopoietin-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Caveolae-mediated Tie2 signaling contributes to CCM pathogenesis in a brain endothelial cell-specific Pdcd10-deficient mouse model
Authors: HJ Zhou, L Qin, Q Jiang, KN Murray, H Zhang, B Li, Q Lin, M Graham, X Liu, J Grutzendle, W Min
Nature Communications, 2021-01-25;12(1):504.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Endothelial exocytosis of angiopoietin-2 resulting from CCM3 deficiency contributes to cerebral cavernous malformation.
Authors: Jenny Zhou H, Qin L, Zhang H, Tang W, Ji W, He Y, Liang X, Wang Z, Yuan Q, Vortmeyer A, Toomre D, Fuh G, Yan M, Kluger M, Wu D, Min W
Nat Med, 2016-08-22;22(9):1033-1042.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Endothelial cell-derived angiopoietin-2 is a therapeutic target in treatment-naive and bevacizumab-resistant glioblastoma.
Authors: Scholz A, Harter PN, Cremer S et al.
EMBO Mol Med.
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