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Mouse Amphiregulin Antibody

Catalog #: AF989
19 Citations Datasheet
Catalog # Availability Size / Price Qty
AF989
AF989-SP
Detection of Recombinant Human and Mouse Amphiregulin by Western Blot.
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Product Details
Citations (19)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Amphiregulin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Amphiregulin in ELISAs and Western Blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human Amphiregulin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Amphiregulin
Ser94-Lys191
Accession # P31955
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Mouse Amphiregulin Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Amphiregulin Biotinylated Antibody (Catalog # BAF989)

Standard: Recombinant Mouse Amphiregulin Protein (Catalog # 989-AR)

Neutralization
Measured by its ability to neutralize Amphiregulin-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 0.3-1.5 µg/mL in the presence of 50 ng/mL Recombinant Mouse Amphiregulin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Recombinant Human and Mouse Amphiregulin antibody by Western Blot. View Larger

Detection of Recombinant Human and Mouse Amphiregulin by Western Blot. Western blot shows 25 ng of Recombinant Mouse Amphiregulin (Catalog # 989-AR), Recombinant Human Amphiregulin (Catalog # 262-AR), and Recombinant Mouse HB-EGF. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF989) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Amphiregulin at approximately 16-25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunohistochemistry Amphiregulin antibody in Mouse Skin by Immunohistochemistry (IHC-Fr). View Larger

Amphiregulin in Mouse Skin. Amphiregulin was detected in immersion fixed frozen sections of mouse skin using Goat Anti-Mouse Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF989) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Neutralization Cell Proliferation Induced by Amphiregulin and Neutralization by Mouse Amphiregulin Antibody. View Larger

Cell Proliferation Induced by Amphiregulin and Neutralization by Mouse Amphiregulin Antibody. Recombinant Mouse Amphiregulin (Catalog # 989-AR) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Amphiregulin (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF989). The ND50 is typically 0.3-1.5 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Amphiregulin

Amphiregulin (AR), also known as Schwannoma-derived growth factor (SDGF), is a member of the epidermal growth factor (EGF) family of growth factors which includes, AR, EGF, transforming growth factor-a (TGF-a), heparin binding EGF-like growth factor (HB-EGF), betacellulin (BTC), epiregulin, and the neuregulins-1 through -4. All EGF family members are synthesized as type I transmembrane precursors and contain one or several EGF domains in their extracellular region. The bioactive form of the proteins is released by proteolytic cleavage. The ErbB family of receptors that includes ErbB1‑B4, mediates the biological activities of the EGF family ligands. AR was originally isolated from the conditioned media of PMA-treated MCF-7 human breast carcinoma cell line. AR mRNA expression can be detected in numerous carcinoma cell lines and in the epithelial cells of various human tissues including colon, stomach, breast, ovary and kidney. AR stimulates the proliferation of keratinocytes, mammary epithelial cells, fibroblasts, astrocytes and glial cells. AR is also a growth inhibitor for certain tumor cells. The gene for AR has been mapped to human chromosome 4q13-q21 and mouse chromosome 5. Human and mouse AR cDNA encode 252 and 248 amino acid residue type I membrane proteins, respectively. The two proteins share approximately 69% sequence identity. Mouse AR also shares 81% amino acid sequence homology with rat AR. Several secreted isoforms of AR that vary in length and/or glycosylation level can be found in cell conditioned media. The 98 amino acid residue recombinant AR has better receptor binding and biological activity than the C-terminal truncated forms of the protein.

References
  1. Thompson, S.A. et al. (1996) J. Biol. Chem. 271:17927.
  2. Sonoda, H. et al. (1992) Biochem. Biophys. Res. Commun. 185:103.
  3. Normanno, N. et al. (2001) Frontiers in Bioscience 6:685.
Entrez Gene IDs
374 (Human); 11839 (Mouse)
Alternate Names
Amphiregulin; AR; AREG; AREGB; Colorectum cell-derived growth factor; CRDGF; MGC13647; schwannoma-derived growth factor; SDGF

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Citations for Mouse Amphiregulin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Mechanisms for Alternaria alternata Function in the Skin During Induction of Peanut Allergy in Neonatal Mice With Skin Barrier Mutations
    Authors: Lauren M. Buelow, Akihiko Hoji, Kiet Tat, Lindsay M. Schroeder-Carter, Daniela J. Carroll, Joan M. Cook-Mills
    Frontiers in Allergy
  2. Mammary tumorigenesis induced by fibroblast growth factor receptor 1 requires activation of the epidermal growth factor receptor
    Authors: Lindsey K. Bade, Jodi E. Goldberg, Hazel A. DeHut, Majken K. Hall, Kathryn L. Schwertfeger
    Journal of Cell Science
  3. Notch4 signaling limits regulatory T-cell-mediated tissue repair and promotes severe lung inflammation in viral infections
    Authors: Hani Harb, Mehdi Benamar, Peggy S. Lai, Paola Contini, Jason W. Griffith, Elena Crestani et al.
    Immunity
  4. Compatibility of intravitreally applied epidermal growth factor and amphiregulin
    Authors: Mukharram M. Bikbov, Timur A. Khalimov, Marc Cerrada-Gimenez, Symantas Ragauskas, Giedrius Kalesnykas, Jost B. Jonas
    International Ophthalmology
  5. The GABAA Receptor Influences Pressure Overload-Induced Heart Failure by Modulating Macrophages in Mice
    Authors: Jin Bu, Shiyuan Huang, Jue Wang, Tong Xia, Hui Liu, Ya You et al.
    Frontiers in Immunology
  6. Tissue remodeling by an opportunistic pathogen triggers allergic inflammation
    Authors: Karen Agaronyan, Lokesh Sharma, Bharat Vaidyanathan, Keith Glenn, Shuang Yu, Charles Annicelli et al.
    Immunity
  7. GPR174 knockdown enhances blood flow recovery in hindlimb ischemia mice model by upregulating AREG expression
    Authors: J Liu, L Pan, W Hong, S Chen, P Bai, W Luo, X Sun, F He, X Jia, J Cai, Y Chen, K Hu, Z Song, J Ge, A Sun
    Nature Communications, 2022-12-06;13(1):7519.
    Species: Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Neuropeptide regulation of non-redundant ILC2 responses at barrier surfaces
    Authors: AM Tsou, H Yano, CN Parkhurst, T Mahlakõiv, C Chu, W Zhang, Z He, KJ Jarick, C Zhong, GG Putzel, M Hatazaki, JRI IBD Li, IC Lorenz, D Andrew, P Balderes, CSN Klose, SA Lira, D Artis
    Nature, 2022-11-02;0(0):.
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Proenkephalin+ regulatory T cells expanded by ultraviolet B exposure maintain skin homeostasis with a healing function
    Authors: H Shime, M Odanaka, M Tsuiji, T Matoba, M Imai, Y Yasumizu, R Uraki, K Minohara, M Watanabe, AJ Bonito, H Fukuyama, N Ohkura, S Sakaguchi, A Morita, S Yamazaki
    Proc. Natl. Acad. Sci. U.S.A., 2020-08-07;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC, Neutralization
  10. Deoxycholic acid activates epidermal growth factor receptor and promotes intestinal carcinogenesis by ADAM17-dependent ligand release
    Authors: W Dong, L Liu, Y Dou, M Xu, T Liu, S Wang, Y Zhang, B Deng, B Wang, H Cao
    J. Cell. Mol. Med., 2018-06-29;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
  11. Amphiregulin contained in NSCLC-exosomes induces osteoclast differentiation through the activation of EGFR pathway
    Authors: S Taverna, M Pucci, M Giallombar, MA Di Bella, M Santarpia, P Reclusa, I Gil-Bazo, C Rolfo, R Alessandro
    Sci Rep, 2017-06-09;7(1):3170.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  12. A heart-brain-kidney network controls adaptation to cardiac stress through tissue macrophage activation
    Authors: K Fujiu, M Shibata, Y Nakayama, F Ogata, S Matsumoto, K Noshita, S Iwami, S Nakae, I Komuro, R Nagai, I Manabe
    Nat. Med., 2017-04-10;23(5):611-622.
    Species: Mouse
    Sample Types: In Vivo, Whole Tissue
    Applications: IHC, Neutralization
  13. Amphiregulin Antibody and Reduction of Axial Elongation in Experimental Myopia
    Authors: WJ Jiang, HX Song, SY Li, B Guo, JF Wu, GP Li, DD Guo, L Shi, HS Bi, JB Jonas
    EBioMedicine, 2017-02-23;0(0):.
    Species: Guinea Pig
    Sample Types: In Vivo
    Applications: Neutralization
  14. ZEB1 sensitizes lung adenocarcinoma to metastasis suppression by PI3K antagonism.
    Authors: Yang Y, Ahn Y, Chen Y, Tan X, Guo L, Gibbons D, Ungewiss C, Peng D, Liu X, Lin S, Thilaganathan N, Wistuba I, Rodriguez-Canales J, McLendon G, Creighton C, Kurie J
    J Clin Invest, 2014-04-24;124(6):2696-708.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot
  15. Ectodomain shedding of EGFR ligands and TNFR1 dictates hepatocyte apoptosis during fulminant hepatitis in mice.
    Authors: Murthy A, Defamie V, Smookler DS
    J. Clin. Invest., 2010-07-12;120(8):2731-44.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  16. Pubertal exposure to high fat diet causes mouse strain-dependent alterations in mammary gland development and estrogen responsiveness.
    Authors: Olson LK, Tan Y, Zhao Y, Aupperlee MD, Haslam SZ
    Int J Obes (Lond), 2010-03-16;34(9):1415-26.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  17. The Arf-inducing transcription factor Dmp1 encodes a transcriptional activator of amphiregulin, thrombospondin-1, JunB and Egr1.
    Authors: Mallakin A, Sugiyama T, Kai F, Taneja P, Kendig RD, Frazier DP, Maglic D, Matise LA, Willingham MC, Inoue K
    Int. J. Cancer, 2010-03-15;126(6):1403-16.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  18. Wounding-induced synthesis of hyaluronic acid in organotypic epidermal cultures requires the release of heparin-binding egf and activation of the EGFR.
    Authors: Monslow J, Sato N, Mack JA, Maytin EV
    J. Invest. Dermatol., 2009-02-19;129(8):2046-58.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. Genetic interaction implicates iRhom2 in the regulation of EGF receptor signalling in mice
    Authors: Owen M. Siggs, Adam Grieve, Hongmei Xu, Paul Bambrough, Yonka Christova, Matthew Freeman
    Biology Open

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