Monocyte and Dendritic Cell Subsets Flow Cytometry Panel
Monocyte and Dendritic Cell Subsets Flow Cytometry Panel Summary
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Multicolor flow cytometry panel to identify monocyte and dendritic cell subsets. . Monocyte and dendritic cell subsets were determined first based on expression of CD14 and HLA-DR. CD14+ monocytes were then gated into CD16- Classical Monocytes and CD16+ Non-Classical Monocytes. CD14-HLA-DR+ Dendritic Cells were then gated into CD11c+ CD123- monocyte-derived dendritic cells (mDCs) and CD11c-CD123+ plasmacytoid dendritic cells (pDCs). Cells were stained with Anti-Human HLA-DR Alexa Fluor® 405, CD8 FITC, CD11c PE, CD123 PerCP, CD16 APC, CD20 Alexa Fluor 700, CD3 Alexa Fluor 700, CD56 Alexa Fluor 700, and CD14 Alexa Fluor 750. Find Staining Protocol Here.
Background: Flow Cytometry Panel
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