Methylene Blue

Biological Activity

Methylene Blue is a biological stain and redox indicator. Methylene blue inhibits monoamine oxidase A, tau filament formation and nitric oxide synthase (IC₅₀ values = 0.07 μM, 1.9 μM and 5.3 μM, respectively), and inhibits guanylate cyclase. Methylene Blue delays senescence and stimulates proliferation of fibroblasts and skin cells. In vitro, Methylene Blue promotes wound healing, upregulates elastin expression and production, increases synthesis of heme, is neuroprotective (EC₅₀ = 0.18 nM) and rescues the loss of dopaminergic neurons induced by Rotenone (Cat. No. 3616). Also antimalarial.

Technical Data

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

1. Inhibition of heparin-induced tau filament formation by phenothiazines, polyphenols, and porphyrins.
   Taniguchi et al.
   J.Biol.Chem., 2005;280:7614
2. Comparison of two soluble guanylyl cyclase inhibitors, methylene blue and ODQ, on sodium nitroprusside-induced relaxation in guinea-pig trachea.
   Hwang et al.
   Br.J.Pharmacol., 1998;125:1158

Product Datasheets